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Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
Large-volume protein crystals are a prerequisite for neutron diffraction studies and their production represents a bottleneck in obtaining neutron structures. Many protein crystals that permit the collection of high-resolution X-ray...
Fibroblast growth factor-1 (FGF-1) is an angiogenic factor with therapeutic potential for the treatment of ischemic disease. FGF-1 has low intrinsic thermostability and is characteristically formulated with heparin as a stabilizing agent...
Many diseases such as DiGeorge syndrome, leukemia and likely all cancers display abnormal replication timing, the temporal order in which segments of chromosomes are replicated. However, the elements that regulate normal replication...
BACKGROUND: Kallikrein 6 (KLK6) is a newly identified member of the kallikrein family of secreted serine proteases that prior studies indicate is elevated at sites of central nervous system (CNS) inflammation and which shows regulated...
An attempt has been made to improve a crystal contact of human acidic fibroblast growth factor (haFGF; 140 amino acids) to control the crystal growth, because haFGF crystallizes only as a thin-plate form, yielding crystals suitable for X...
The small golden ant Pheidole morrisi Forel is the most common ant of the Apalachicola National Forest, a typical longleaf pine flat-woods ecosystem and is found in pine forests across the eastern U.S. P. morrisi likely plays a...
S(1)' and S(2)' Subsite Specificities of Human Plasma Kallikrein and Tissue Kallikrein 1 for the Hydrolysis of Peptides Derived from the Bradykinin Domain of Human Kininogen
The S(1)' and S(2)' subsite specificities of human tissue kallikrein 1 (KLK1) and human plasma kallikrein (HPK) were examined with the peptide series Abz-GFSPFRXSRIQ-EDDnp and Abz-GFSPFRSXRIQ-EDDnp [X=natural amino acids or S(PO(3)H(2))]...
While protease-activated receptors (PARs) are known to mediate signaling events in CNS, contributing both to normal function and pathogenesis, the endogenous activators of CNS PARs are poorly characterized. In this study, we test the...
Kallikrein-related peptidase 6 (KLK6) is a trypsin-like serine protease upregulated at sites of central nervous system (CNS) injury, including de novo expression by reactive astrocytes, yet its physiological actions are largely undefined...
Autosomal recessive primary microcephaly (MCPH, MIM 251200) is a neurodevelopmental disorder that results from a loss of neural progenitors in the embryonic neocortex. Patients with MCPH have a significantly small brain and exhibit...
The interaction between various signaling pathways and their spatial and temporal regulation during growth and development remains largely unknown. The purpose of this thesis is to investigate the role of signaling pathways involved in...
In Drosophila melanogaster, cells that are normally viable in homogeneous tissue can be eliminated when surrounded by cells with a different level of expression of a certain gene, such a phenomenon has been termed cell competition. A...
Transition zones are regions in the animal body where two types of epithelial tissue meet. Many transition zones are known high-risk sites for tumorigenesis. However, little is known on why transition zones are more susceptible to tumor...
The rabbit is an important and de facto animal model in the study of ischemic disease and angiogenic therapy. Additionally, fibroblast growth factor 1 (FGF-1) is emerging as one of the most important growth factors for novel...
The mobile and self-perpetuating nature of transposons allows them to impact organisms in a polarized manner: as an evolutionary force in increasing genome size and as molecular parasites whose activities may lead to potentially...
During the age of non-avian dinosaurs, ancestors of present-day mammals were likely small insectivores, relegated to nocturnal and subterranean niches. This nocturnal "bottle-neck" is postulated as a driving force of numerous...
Cutaneous malignant melanoma is an aggressive disease of poor prognosis. Clinical and experimental studies have provided major insight into the pathogenesis of the disease, including the functional interaction between melanoma cells and...
Tissue kallikrein KLK1 and the kallikrein-related peptidases KLK2-15 are a subfamily of serine proteases that have defined or proposed roles in a range of central nervous system (CNS) and non-CNS pathologies. To further understand their...
We previously reported the activation profiles of the human kallikrein-related peptidases (KLKs) as determined from a KLK pro-peptide fusion-protein system. That report described the activity profiles of 12 of the 15 mature KLKs versus...
The precise regulation of cellular and hormonal signaling pathways is required for proper development in metazoan organisms. The Drosophila imaginal wing disc is the ideal model system for the study of cellular signaling regulating...
The human kallikrein-related peptidases (KLKs) comprise 15 members (KLK1-15) and are the single largest family of serine proteases. The KLKs are utilized, or proposed, as clinically important biomarkers and therapeutic targets of...
A large body of emerging evidence indicates a functional interaction between the kallikrein-related peptidases (KLKs) and proteases of the thrombostasis axis. These interactions appear relevant for both normal health as well as...
The human tissue kallikrein (KLK) family contains 15 secreted serine proteases that are expressed in a wide range of tissues and have been implicated in different physiological functions and disease states. Of these, KLK1 has been shown...
Abstract The 15 human kallikrein-related peptidases (KLKs) are clinically important biomarkers and therapeutic targets of interest in inflammation, cancer, and neurodegenerative disease. KLKs are secreted as inactive pro-forms (pro-KLKs)...
The follicular epithelium (FE) of the Drosophila egg chamber is an excellent model system to study cell-cycle regulation, cell differentiation and cell migration in development. During oogenesis, follicle cells sequentially undergo three...
In previous work, Striegel and Hurdal have developed a mathematical model for cortical folding pattern formation during development (Striegel). A Turing reaction-diffusion system and a prolate spheroid domain were used to model the shape...
The majority of protein architectures exhibit elements of structural symmetry, and "gene duplication and fusion" is the evolutionary mechanism generally hypothesized to be responsible for their emergence from simple peptide motifs....
Cellular signaling pathways constitute series of genes widely used in a multitude of molecular processes within an organism. Generally, the activation of these pathways is relatively upstream in the sequence of developmental events...
Human kallikrein-related peptidase 6 (KLK6) is a member of the kallikrein family of serine-type proteases, characterized as an arginine-specific digestive-type protease capable of degrading a wide-variety of extracellular matrix proteins...
Type 2 diabetes, the more prevalent form of diabetes mellitus, is caused by insulin resistance in peripheral tissues due to a post-receptor defect in the insulin-signaling pathway. Binding of insulin to its receptor in the cell membrane...
Drosophila melanogaster serve as an excellent model to study the highly conserved notch signaling pathway, which is involved in a broad array of developmental events. The Notch signal transduction pathway in Drosophila is vastly complex...
Mitochondria, important energy centers in the cell, also control sperm cell morphogenesis.Drosophila spermatids have a remarkably large mitochondrial formation called the nebenkern. Immediately following meiosis during sperm development, ...
The neurophysiological foundation and anatomical basis of circuits necessary for adult behaviors are established early in development. Numerous perinatal and postnatal variables affect circuit trajectory during developmental sensitive...
Characterizing Non-Typical Epithelial Behavior: Analysis of the Spatiotemporal Patterns of Patency, and a Study of Nonautonomous Cellular Compaction in Drosophila Melanogaster Follicular Epithelium
The follicular epithelium (FE) in the Drosophila melanogaster ovary is an excellent model system to study epithelial processes and behaviors, such as cell-cell communication, cell cycle regulation, and epithelial morphogenesis. The...
Improving the Accuracy of 3D Chromosome Structure Inference and Analyzing the Organization of Genome in Early Embryogenesis Using Single Cell Hi-C Data
This dissertation summarizes my graduate work on the structure and organization of mouse genome during preimplantation development. My research is divided into three different areas, which I will discuss in turn. To begin, I will discuss...
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