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Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
Diphtheria toxin repressor is a regulatory protein from Corynebacterium diphtheriae, the causal agent of Diphtheria. The diphtheria toxin repressor (DtxR) contains an SH3-like domain that forms an intramolecular complex with a proline...
Tissue engineering is one of the fastest growing biotechnology industries. The overarching research goals of the tissue engineering industry are highly intertwined with the field of medicine. The tissue engineering field seeks to create...
Developing functional inorganic nanocrystals for biomedical uses, such as cellular labeling, bio-imaging and optical sensing, has attracted great momentum and many activities in both academia and industry over the past two decades. Many...
Removal of introns from pre-mRNA in eukaryotes is mediated by the spliceosome, a dynamic supramolecular complex comprising five small nuclear (sn)RNAs and numerous proteins, organized into small ribonucleoprotein (snRNP) particles. At...
Matrix metalloproteinases (MMPs), a family of enzymes known for their proteolytic activities on processing extracellular matrix substrates, may play an integral role in blood-brain barrier opening following an ischemic stroke. Several...
Over half of the proteins found in nature are anticipated to be glycosylated, yet less than 2% of the protein structures in the protein data bank are glycoproteins. Clearly, we are behind in our structural understanding of glycoproteins....
Over the last decades, the enzyme Glucokinase (GCK) has interested biochemists due to its physiological and kinetic characteristics. GCK is vital for glucose homeostasis, as it controls the levels of insulin and glycogen synthesis in the...
Prostate cancer is the most prevalent cancer diagnosed among men in the United States. The major green tea polyphenol epigallocatechin-3 gallate (EGCG) has been shown to exert remarkable preventive effects against various types of cancer...
Use of Inorganic Quantum Dot-Cationic Liposome Hybrids for the Delivery and Expression of Calcium-Sequestering Parvalbumin into Mammalian Cell Cultures
Left Ventricular Diastolic Dysfunction is one of the main causes of Heart Failure. It is caused by a defect in the relaxation of cardiac muscle usually as the result of failure of the heart cells to remove cytoplasmic Ca2+ following...
Nucleic acids are the fundamental building blocks of the genetic systems of all living organisms. The structural conformation and function of nucleic acids are determined by the balance among a lot of factors. This work examines two of...
We describe a cryo-electron microscopy 3D image reconstruction of relaxed myosin II-containing thick filaments from the flight muscle of the giant waterbug Lethocerus indicus. The relaxed thick filament structure is a key element of...
The Iron-dependent Regulator (IdeR) is a 230-amino acid transcriptional repressor that regulates iron homeostasis, oxidative stress response and virulence in Mycobacterium tuberculosis. The importance of IdeR for mycobacterial metabolism...
Molecular modeling of proteins and DNA is an attractive goal because it allows to gain insight into dynamic behavior of molecules on atomistic level. Such studies have a great potential to complement existing experimental techniques in...
Thy-1 is being pursued as a model system for NMR studies of structure and dynamics of intact glycoproteins. However, the low expression yield of the Thy-1 EGFP glycoprotein in insect and mammalian cells has been an obstacle for these...
Liquid chromatography is ubiquitous, but two-dimensional liquid chromatography is rare. The difference is in the difficulty of method development, as once floated variables have increased pertinence. Three issues for method development...
In aqueous solution, solute conformational transitions are governed by intimate interplays of the fluctuations of solute–solute, solute–water, and water–water interactions. To more effectively sample conformational transitions in aqueous...
The unique chemical, optical and physical properties of inorganic nanocrystals have generated tremendous interest to develop a variety of applications, most importantly as novel probes in biology. Recent developments have advertised them...
This work discusses the structure, function, activation and inhibitor screening of human kallikrein 1. Human kallikrein 1 is the first member of the kallikrein family and is involved in blood pressure regulation. hK1 exhibits unique dual...
Modern protein catalysts are often viewed as possessing exquisite specificities for their cognate physiological substrates. In contrast, primordial catalysts are thought to have possessed much broader substrate specificities, a...
Monoclonal antibody (mAb) A20 is the best characterized AAV2 neutralizing mAb [18, 116, 165]. Large quantities of the mAb A20 were obtained from hybridomas cell culture, and A20 Fab fragments were produced and purified by standard...
Residing in a host of tissues, including skin, muscle, liver, heart, and even hair follicles, non-embryonic stem cells, which are gaining a popularity rivaling that of their embryonic precursors, are capable of combating a number of...
With the development of ionization techniques such as electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI), mass spectrometry expands its subject of study from small volatile molecules to non-volatile...
Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a...
Pinnatoxin A, a marine-derived macrocycle originating from a bivalve within the shellfish Pinna muricata and Pinna attenuata, was first isolated in 1995 by Uemura and has been a target of several synthetic studies due to its intriguing...
Muscle plays a primary role in movement of the body of multicellular organs. A study of muscle contraction at a molecular level will provide understanding of muscular malfunction, as well as insights into the basic mechanism of...
RNA-RNA interactions involved in recognition associated with ribozyme catalysis are essential for ribozyme function. Research described in this dissertation focuses on RNA-RNA interactions at, and in the vicinity of two splice sites of...
The Adeno-Associated Virus has moved to the forefront as a vector for human gene therapy. Vectors have been constructed with AAV to repair many genetic deficiencies such as cystic fibrosis and hemophilia and have demonstrated remarkable...
Orientational restraints resulting from a wide range of anisotropic nuclear spin interactions such as chemical shifts, heteronuclear dipolar interactions, and quadrupolar interactions, have been widely used for determining the structures...
Murine adenosine deaminase (ADA), a 40 kDa enzyme, catalyzes the hydrolytic deamination of adenosine to form inosine, enhancing the rate of the spontaneous reaction by more than than 1011 times the rate of the non-catalyzed reaction....
Cargo trafficking using vesicles has been studied in eukaryotic cells for more than half a century. Three conventional and evolutionarily archaic pathways have since been identified. These pathways include: Coat Protein II (COPII), Coat...
We use a lipid monolayer system to prepare paracrystalline rafts of F-actin with two different cross-linking proteins, α-actinin and villin. α-Actinin cross-links F-actin into relatively loose networks throughout cells, while villin...
RNA-Metal Ion Interactions and Metal Ion-Induced Confromational Change in the Spliceosomal U2-U6 Snrna Complex Studied by Lanthanide Ion Luminescnece and Resonance Energy Transfer Techniques
RNA splicing is an integral step of gene expression. In eukaryotic cells, it is carried out by a dynamic RNA-protein assembly called the spliceosome. Among its RNA components, U2 and U6 snRNAs are most conserved and form a complex with...
Under normal physiological conditions, mature human coronary artery smooth muscle cells (hCASMCs) exhibit a 'contractile' phenotype marked by low rates of proliferation, migration, and protein synthesis, but these cells possess the...
Non-canonical forms of DNA like the guanine quadruplex (G4) play important roles in regulating transcription and translation through interactions with their protein partners. G4s comprise a class of nucleic acid structures formed by...
The metabolic makeup of a biological system is a key determinant of its biological state providing detailed insights into its function. Identification and quantification of the metabolites in a system form critical components of...
Copper transporting ATPase (Cu-ATPase) is a member of the P1B-subfamily of P-type ATPases, which catalyzes ATP-dependent copper delivery across cellular membranes. The energy derived from ATP is used in the active transport of Cu+...
3D Molecular Cytological and Genetic Analysis of the SUN-Domain (Sad1-Unc-84) Proteins in Maize (Zea Mays, L.) Meiosis; Discovery of a Novel Plant Sun Family, Including SUN3, a Candidate Gene for the Desynaptic1 (dy1) Mutant
Meiosis is the process by which sexually reproducing organisms reduce their genomes from diploid (2n) to haploid (n) during the formation of gametes. It requires that homologous chromosomes pair, synapse, recombine, and finally segregate...
Human matrix metalloproteinases-26 (MMP-26/endometase/matrilysin-2) is a newly identified MMP. MMP-26 has one high- and low-affinity calcium binding sites, C1 and C3, respectively, and loss of low-affinity Ca2+ induces tertiary...
Myosin V, as a member of the myosin family, can "walk" along F-actin to transport many "cargos", such as mRNA and secretary vesicles, to its destination. This study is part of a research project that aims to understand the interaction...
Cryo-electron microscopy (cryo-EM) has become a prevailing technique at the interface of structural and cell biology. The current resolution revolution in cryo-EM has led to a surge of structures determination at near-atomic resolution....
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