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AN EVALUATION OF THE RELATIVE EFFECTIVENESS OF EXTENSION WORK UNDER TWO DIFFERENT SYSTEMS OF PERSONNEL ASSIGNMENT IN SELECTED INDIANA COUNTIES
Title
AN EVALUATION OF THE RELATIVE EFFECTIVENESS OF EXTENSION WORK UNDER TWO DIFFERENT SYSTEMS OF PERSONNEL ASSIGNMENT IN SELECTED INDIANA COUNTIES.
Creator
MCINTYRE, WILLIAM JAMES., The Florida State University
Date Issued
1968, 1968
Identifier
AAI6916387, 2985968, FSDT2985968, fsu:70477
Format
Document (PDF)
IMPROVING AGRICULTURAL EDUCATION IN ZAIRE: A STRATEGY FOR EDUCATIONAL PLANNING
Title
IMPROVING AGRICULTURAL EDUCATION IN ZAIRE: A STRATEGY FOR EDUCATIONAL PLANNING.
Creator
CHIRUME, MENDO RUTEBUKA., The Florida State University
Date Issued
1979, 1979
Identifier
AAI7926731, 2989159, FSDT2989159, fsu:73666
Format
Document (PDF)
A CROSS-SECTION ANALYSIS INTEGRATING NUTRITIONAL VARIABLES WITH THE ECONOMIC RELATIONSHIPS OF DEVELOPING COUNTRIES
Title
A CROSS-SECTION ANALYSIS INTEGRATING NUTRITIONAL VARIABLES WITH THE ECONOMIC RELATIONSHIPS OF DEVELOPING COUNTRIES.
Creator
SCHMITT, BERNARD ALOYSIUS., The Florida State University
Date Issued
1977, 1977
Identifier
AAI7731040, 2988712, FSDT2988712, fsu:73219
Format
Document (PDF)
THE PRODUCT AND UTILIZATION ALTERNATIVES FOR BARK IN FLORIDA AND THE COSTS OF BARK PROCESSING OPERATIONS
Title
THE PRODUCT AND UTILIZATION ALTERNATIVES FOR BARK IN FLORIDA AND THE COSTS OF BARK PROCESSING OPERATIONS.
Creator
DONAHUE, JEFFREY CORNELIUS., The Florida State University
Date Issued
1972, 1972
Identifier
AAI7311317, 2987160, FSDT2987160, fsu:71669
Format
Document (PDF)
Florida farm
Title
A Florida farm.
Creator
Whitmore, Frederic., Whitmore, William R.
Abstract/Description
"Reprinted from the Atlantic monthly with additions."
Date Issued
1903
Identifier
03032158, AAB1921QF00008/04/200403/24/200613947BfamI D0QF, 01550469, FS00000207, 2686266, fsu:96210
Format
E-book
Florida, an advancing state, 1907-1917-1927.
Title
Florida, an advancing state, 1907-1917-1927.
Creator
Florida Legislature
Date Issued
1928
Identifier
2666674, FSDT2666674, fsu:36106
Format
E-book
Education, agricultural extension and peasant farmer marginalization: A case study in the high Amazon of Peru
Title
Education, agricultural extension and peasant farmer marginalization: A case study in the high Amazon of Peru.
Creator
Dall, Frank Patrick., Florida State University
Abstract/Description
This educational ethnography describes and examines the training process of the World Bank's sponsored "Train and Visit" agricultural extension method which was introduced to the Peruvian jungle department of San Martin in 1980. The T&V training service attempts to transfer new crop growing technologies to a group of 46 small-scale subsistence and commercial farmers who make up the network of T&V's "contact farmers" in the Bellohorizonte sector of Tarapoto's Agricultural Extension Agency....
Show moreThis educational ethnography describes and examines the training process of the World Bank's sponsored "Train and Visit" agricultural extension method which was introduced to the Peruvian jungle department of San Martin in 1980. The T&V training service attempts to transfer new crop growing technologies to a group of 46 small-scale subsistence and commercial farmers who make up the network of T&V's "contact farmers" in the Bellohorizonte sector of Tarapoto's Agricultural Extension Agency. Farmers who participated in this study live in remote high-jungle valleys of the San Martin province located in Peru's northeastern Amazon region. They also play an important "contact" role in transmitting agricultural information to neighboring farmers who are the beneficiaries in the "modernization" process observed., Using ethnographic field research methods, this case study tries to reveal the intended and unintended socio-cultural effects produced by the local T&V farmer extension training methodology on some of the "marginalized" farmers of this jungle milieu. Farmers of this area are said to be "marginalized" because they lack access to education, health, arable land, water, credit, and other necessary life-supporting services., A closer exmination of the interaction of cultural, socio-economic and political factors on the local farmer training process identified important cultural and technical factors which could be included in the redesign of a more appropriate farmer education model for the Peruvian high Amazon., A major finding of this study is that considerable unplanned learning (informal learning) has already taken place through long-established culturally defined channels, like "reciprocal labor groups" and "father-to-son apprenticeships," and that these may be playing a role in the small-scale farmer development process. As part of this thesis, efforts were made to make "visible" these traditional learning activities and show how they may relate to formal education and contextual factors like each farmer's previous work experience, work style, geographic origin and the availability of important agricultural inputs like land, water, and credit.
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Date Issued
1987, 1987
Identifier
AAI8817360, 3161594, FSDT3161594, fsu:77795
Format
Document (PDF)
FOREST TECHNOLOGY: SELECTED TWO-YEAR PROGRAMS AND THEIR GRADUATES
Title
FOREST TECHNOLOGY: SELECTED TWO-YEAR PROGRAMS AND THEIR GRADUATES.
Creator
BACKELS, JOHN FRANZ., The Florida State University
Date Issued
1968, 1968
Identifier
AAI6900584, 2985853, FSDT2985853, fsu:70362
Format
Document (PDF)
BIOLOGICAL AND BIOCHEMICAL RELATIONSHIPS OF A RICKETTSIALIKE BACTERIUM TO PHONY PEACH DISEASE
Title
BIOLOGICAL AND BIOCHEMICAL RELATIONSHIPS OF A RICKETTSIALIKE BACTERIUM TO PHONY PEACH DISEASE.
Creator
STASSI, DIANE LYNN., The Florida State University
Date Issued
1978, 1978
Identifier
AAI7909805, 2989051, FSDT2989051, fsu:73558
Format
Document (PDF)
FEMALE STERILITY IN CITRUS
Title
FEMALE STERILITY IN CITRUS.
Creator
JACKSON, LARRY KEITH., The Florida State University
Date Issued
1972, 1972
Identifier
AAI7300572, 2987069, FSDT2987069, fsu:71578
Format
Document (PDF)
INDOLE-3-ACETIC ACID OXIDATION AND MULTIPLE PEROXIDASES IN RELATION TO MATURATION IN PITH OF TOBACCO (NICOTIANA TABACUM L.)
Title
INDOLE-3-ACETIC ACID OXIDATION AND MULTIPLE PEROXIDASES IN RELATION TO MATURATION IN PITH OF TOBACCO (NICOTIANA TABACUM L.).
Creator
KERSTETTER, REX EUGENE., The Florida State University
Date Issued
1967, 1967
Identifier
AAI6800364, 2985710, FSDT2985710, fsu:70219
Format
Document (PDF)
"It's All about the Work"
Title
"It's All about the Work": Production, Consumption, and Meaning Making at the Modern Farmers Market.
Creator
Lennon, Lindsey, Schrock, Douglas P., Broome, Jeffrey L. (Jeffrey Lynn), Reynolds, John K., Ramirez, Hernan, Florida State University, College of Social Sciences and Public...
Show moreLennon, Lindsey, Schrock, Douglas P., Broome, Jeffrey L. (Jeffrey Lynn), Reynolds, John K., Ramirez, Hernan, Florida State University, College of Social Sciences and Public Policy, Department of Sociology
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Abstract/Description
Farmers markets surged in popularity in the United States over the past two decades. The negative consequences of large-scale, industrialized agriculture and changing consumer interests together stimulated this drive towards alternative farming practices and local, direct-from-producer foods. As a result, farmers markets created the opportunity for new, small-scale producers to enter the marketplace and make a living off the land. The need persists to explore the social aspects of this food...
Show moreFarmers markets surged in popularity in the United States over the past two decades. The negative consequences of large-scale, industrialized agriculture and changing consumer interests together stimulated this drive towards alternative farming practices and local, direct-from-producer foods. As a result, farmers markets created the opportunity for new, small-scale producers to enter the marketplace and make a living off the land. The need persists to explore the social aspects of this food phenomenon from the perspective of the farmer. Using in-depth interviews with farmers and fieldwork at several farmers markets, I examine how producers became and remained vendors at farmers markets. In the first empirical analysis (Chapter 4), I use farmers' retrospective accounts to outline the ways farming was discovered and the indirect and lengthy process of becoming a farmers market vendor. Along with market opportunity, I find that this process was dependent on a combination of social support, personal determination and unforeseeable life circumstances. In my second analysis (Chapter 5), I use a combination of interviews, fieldwork, and personal experience as a farmer and vendor to explore the spoken and unspoken requirement for farmers to create an "authentic" farmers market. Farmers' success at the market depended on their ability to create this authentic experience. I find that farmers used strictly enforced market rules and carefully crafted product displays, personal presentations, and social interactions to meet customers' expectations of legitimate farmers and an authentic farmers market. Additionally, I show how these expectations and presentations directly contrasted with the realities of life on the farm. Overall, my research shows how socialized perceptions of farming determined farmers' willingness to adopt the occupation. Once in, farmers sustained their place at the market by meeting customers' expectations and standards of authenticity. Often times this meant farmers had to cover and hide the ugly aspects of their work. These findings show that expectations, meanings, and the social interactions that create and maintain them are import factors to consider in research connecting food production and consumption.
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Date Issued
2016
Identifier
FSU_2016SP_Lennon_fsu_0071E_13152
Format
Thesis
Developing New Datasets to Evaluate Tropospheric Photochemistry and the Effects of Ozone Uptake in the Biosphere
Title
Developing New Datasets to Evaluate Tropospheric Photochemistry and the Effects of Ozone Uptake in the Biosphere.
Creator
Ducker, Jason Alexander, Holmes, Christopher D., Miller, Thomas E., Misra, Vasubandhu, Bourassa, Mark Allan, Florida State University, College of Arts and Sciences, Department...
Show moreDucker, Jason Alexander, Holmes, Christopher D., Miller, Thomas E., Misra, Vasubandhu, Bourassa, Mark Allan, Florida State University, College of Arts and Sciences, Department of Earth, Ocean, and Atmospheric Science
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Abstract/Description
In the presence of water vapor, photolysis of tropospheric ozone (O3) produces the hydroxyl radical (OH), which is a strong oxidant that directly and indirectly controls a host of greenhouse gases and air pollutants. When tropospheric O3 reaches the surface, its oxidative effects perturb plant transpiration and photosynthesis. Although these effects have been included in climate and air quality models, there are limited observational datasets to constrain key aspects of atmospheric...
Show moreIn the presence of water vapor, photolysis of tropospheric ozone (O3) produces the hydroxyl radical (OH), which is a strong oxidant that directly and indirectly controls a host of greenhouse gases and air pollutants. When tropospheric O3 reaches the surface, its oxidative effects perturb plant transpiration and photosynthesis. Although these effects have been included in climate and air quality models, there are limited observational datasets to constrain key aspects of atmospheric photochemistry and O3 deposition on regional to global scales. This dissertation develops and uses two new datasets to better understand the ozone photochemistry and impacts. Photolysis, the breaking of chemical bonds by sunlight, is the engine for reactive atmospheric chemistry. It controls production of atmopsheric oxidants, especially O3 and OH, which then influence the lifetimes of other air pollutants and climate forcing agents. Global chemistry and climate models differ in their estimates of these photolysis rates and there have been datasets capable of discriminating among different models. Here, we integrate satellite-retrivals of clouds and aerosols into a photolysis code and produce a 3-D global photolysis dataset called Sat-J. We show that Sat-J is tightly correlated with in-situ measurements of pholysis rates from airborne chemistry campaigns, with errors (4-20%) mainly attributed to differences in nonuniform cloud sampling and time match differences. By comparing regional, not necessarily collocated, averages of aircraft data, SatJ, and a chemistry model (GEOS-Chem); we demonstrate that SatJ provides a representative climatology of photolysis rates across the globe and can serve as a benchmark for photochemistry models. Using surface micrometeorological fluxes and surface O3 monitoring networks, we also develop and evaluat a method to estimate O3 deposition and stomatal O3 uptake across networks of eddy covariance flux tower sites where O3 concentrations and O3 fluxes have not been measured. This method, called SynFlux, reproduces the variability in daily stomatal O3 uptake at sites with O3 flux measurements, with a modest bias (21% or less) attributed to gridded O3 concentrations. Across SynFlux sites, we highlight environmental factors controlling spatial patterns in O3 deposition and showed that previous O3 concentration-based metrics for plant damages did not correlate with SynFlux O3 uptake, which is a better predictor for plant damage than ambient concentration in air. SynFlux has dramatically expanded the the available data on surface O3 deposition, which can now be used for performing ecosystem impact studies across a species and climates in the US and Europe. Past controlled experiments involving single plant species have shown that O3 uptake can degrade water-use efficiency (WUE), which is the ratio of carbon uptake in photosynthesis (GPP) to water loss in plant transpiration (T). Using SynFlux sites, we can quantify this effect for whole ecosystems under natural environmental variability, which has not been previously studied. Across 74 SynFlux sites, we find a significant negative relationship (–0.02% per μmol m-2 d-1) between daily cumulative O3 uptake (CUO) and WUE anomalies, with the largest impacts occurring at forest sites. Past controlled studies of selected individual species also observed a similar O3 reduction of WUE over the growing season, indicating a consistent response to O3 across multiple species with an ecosystem. When we analyze the relationships between daily CUO and GPP or T anomalies, we also find that CUO degrades GPP and increases T over the growing season. We postulate that O3 degrades WUE through O3 non-stomatal biochemical factors, which result in a reduction of GPP or an increase in T. Our SynFlux results here provide climate models the ability to incorporate O3-dose response relationships between O3 uptake and ecosystem carbon and water vapor fluxes across ecosystems that have not previously been studied. For chapters 2-4, we have separate supplementary documents for each chapter.
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Date Issued
2019
Identifier
2019_Fall_Ducker_fsu_0071E_15515_P
Format
Set of related objects
Conserving forest diversity in northern Florida: From landscapes to populations
Title
Conserving forest diversity in northern Florida: From landscapes to populations.
Creator
Schwartz, Mark William., Florida State University
Abstract/Description
I examine three aspects forest vegetation of northern Florida: the Land Office Survey records of the abundance and distribution of woody species across northern Florida in the early nineteenth century; the abundance and distribution of woody species across a sharp elevation gradient in the hardwood slope forests along the Apalachicola River; and the population decline of Torreya taxifolia., Land Office Survey data was tabulated for all available bearing trees north of Gainesville. This study...
Show moreI examine three aspects forest vegetation of northern Florida: the Land Office Survey records of the abundance and distribution of woody species across northern Florida in the early nineteenth century; the abundance and distribution of woody species across a sharp elevation gradient in the hardwood slope forests along the Apalachicola River; and the population decline of Torreya taxifolia., Land Office Survey data was tabulated for all available bearing trees north of Gainesville. This study includes 131,227 trees sampled at 49,896 section corners and mid-point lines. This data tabulation shows that northern Florida was dominated by pines prior to U.S. settlement. Over 77 percent of bearing trees were pines. Pineland oaks are the next most abundant species after pine. Wetlands trees, such as bay, gum, and cypress were dominant species in the absence of pine. The mixed hardwood species are a diverse group, including beech, magnolia, and dogwood, with no clear dominant among them., The second portion of this research examines the spatial relationships among the species of the hardwood slope forests along the Apalachicola River. Species distributions were sampled using 80 belt transects in four ravine systems. Species turnover across elevation is high, but measures of species diversity and forest structure do not change with elevation. Distribution patterns of the major species indicate that assemblages in the adjacent elevation samples in the middle and upper slope positions are more similar to one another than are assemblages from the lower elevations. The assemblages do not, however, appear to form sub-communities with respect to elevation., Finally, I examine hypotheses for the decline of Torreya taxifolia, a narrowly endemic conifer native to ravines along the Apalachicola River. Included in this analysis of the torreya decline is a demographic summary of 108 torreya on preserve land. Most individual torreya are multiple stemmed and less than 14 years old. Further, these individuals are most frequently grown at low elevations and under moderate to dense canopy shade. Among the many hypotheses proposed to explain the decline, I present evidence that fire suppression may have allowed fungal populations to soar, contributing to the onset of disease.
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Date Issued
1990, 1990
Identifier
AAI9113950, 3162205, FSDT3162205, fsu:78403
Format
Document (PDF)
Adult education and the cross-cultural transfer of innovation: A critical analysis of the Rural Organizations Development policy in Niger
Title
Adult education and the cross-cultural transfer of innovation: A critical analysis of the Rural Organizations Development policy in Niger.
Creator
Sidikou, Maman Sambo., Florida State University
Abstract/Description
Development assistance has long served as pretext for the transfer of technologies, policies, and strategies from Western industrialized countries to poorer areas of the world. The assumption is that the same successful results reached in the originating context can be replicated. One such cross-cultural transfer is a US adult education policy implemented in the rural areas of the Republic of Niger., The policy, known as ROD, purports to transform local cooperatives into profit-making private...
Show moreDevelopment assistance has long served as pretext for the transfer of technologies, policies, and strategies from Western industrialized countries to poorer areas of the world. The assumption is that the same successful results reached in the originating context can be replicated. One such cross-cultural transfer is a US adult education policy implemented in the rural areas of the Republic of Niger., The policy, known as ROD, purports to transform local cooperatives into profit-making private enterprises, and to teach farmers the requisite skills and understandings. This study critically analyzes the intervention in order to (1) assess the viability of the proposed strategy as a "curriculum" for personal and organizational learning in rural Niger, (2) explicate the assumptions about the problems and potentials of the milieu on which it is based, (3) explore ways in which revised adult education practice might serve to better accommodate the policy to its context, and (4) draw some conclusions regarding the feasibility of this sort of transfer., Results of the study suggest that the intervention strategy is interpreted and assessed in very different ways by stakeholders at different levels, and that it has been modified significantly in the course of implementation. These mostly haphazard modifications have not, however, been such as to ensure its success or acceptance, though they do suggest graphically which aspects of the policy are least accepted in the local context. The data also provide a basis for suggesting ways in which adult education could facilitate a better upfront adaptation and "reinvention" of the strategy in the field. In a more general sense, this study provides insights into the conditions for cross-cultural transfer of adult education-relevant policies between North and South, and the precautions that must be taken.
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Date Issued
1994, 1994
Identifier
AAI9434118, 3088407, FSDT3088407, fsu:77212
Format
Document (PDF)
The effects of hurricane winds and associated salt spray on the growth and developmental anatomy of secondary xylem in slash pine (Pinus elliottii) from Cape Saint George Island, Franklin County, Florida, United States of America
Title
The effects of hurricane winds and associated salt spray on the growth and developmental anatomy of secondary xylem in slash pine (Pinus elliottii) from Cape Saint George Island, Franklin County, Florida, United States of America.
Creator
Alden, Harry Arthur., Florida State University
Abstract/Description
Trees of Slash pine showed reduced growth, by an order of magnitude, following exposure to two hurricanes (Elena and Kate) in 1985. The reduced growth is caused by salt spray chlorosis and/or defoliation from high winds. Ring width, radial tracheid diameter, radial cell number, tracheid length and microfibril angle were measured for samples from before and after major storms. Data for ring width, earlywood radial cell diameter and cell number were not normally distributed, while data for...
Show moreTrees of Slash pine showed reduced growth, by an order of magnitude, following exposure to two hurricanes (Elena and Kate) in 1985. The reduced growth is caused by salt spray chlorosis and/or defoliation from high winds. Ring width, radial tracheid diameter, radial cell number, tracheid length and microfibril angle were measured for samples from before and after major storms. Data for ring width, earlywood radial cell diameter and cell number were not normally distributed, while data for latewood radial cell diameter, tracheid length and microfibril angle showed normal distributions. There were significant statistical differences for all main effects for ring width, earlywood radial cell diameter, cell number and tracheid length. The data for latewood radial cell diameter show significant differences for effects of time and height, but not for compass direction. There are significant differences in earlywood cellulose microfibril angle for height effects, and no difference for time and direction effects. Data for latewood cellulose microfibril angle show significant differences for the effects of time and height.
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Date Issued
1993, 1993
Identifier
AAI9416140, 3088288, FSDT3088288, fsu:77092
Format
Document (PDF)
HYDROCARBON FORMATION FROM MODEL SYSTEMS AND FREEZE-DRIED MEATS
Title
HYDROCARBON FORMATION FROM MODEL SYSTEMS AND FREEZE-DRIED MEATS.
Creator
SEO, CHUNG-WOON., The Florida State University
Date Issued
1971, 1971
Identifier
AAI7200280, 2986567, FSDT2986567, fsu:71076
Format
Document (PDF)
ANTIOXIDANTS IN BRASSICA RAPA
Title
ANTIOXIDANTS IN BRASSICA RAPA.
Creator
HIRANO, YOSHIO., The Florida State University
Date Issued
1968, 1968
Identifier
AAI6816374, 2985829, FSDT2985829, fsu:70338
Format
Document (PDF)
CATALYSTS OF LIPID PEROXIDATION IN MEATS
Title
CATALYSTS OF LIPID PEROXIDATION IN MEATS.
Creator
LIU, HSIAO-PING., The Florida State University
Date Issued
1968, 1968
Identifier
AAI6911310, 2985922, FSDT2985922, fsu:70431
Format
Document (PDF)
THE EFFECT OF NITROGEN AND POTASSIUM FERTILIZATION ON TOMATO FLAVOR
Title
THE EFFECT OF NITROGEN AND POTASSIUM FERTILIZATION ON TOMATO FLAVOR.
Creator
WRIGHT, DEBORAH HARRIS., Florida State University
Abstract/Description
The effects of nitrogen and potassium fertilization on tomato flavor were studied. Field-grown Walter c.v. tomatoes were treated with three levels of nitrogen and potassium. Soluble solids, pH, titratable acidity (TA), volatile flavor compounds, and sensory evaluation of overall flavor were studied., Taste panel tests indicated that the lower fertilization level (100 lb/A N- 150 lb/A K) yielded tomatoes with a more desirable flavor. Tomatoes grown at the two higher levels of fertilization ...
Show moreThe effects of nitrogen and potassium fertilization on tomato flavor were studied. Field-grown Walter c.v. tomatoes were treated with three levels of nitrogen and potassium. Soluble solids, pH, titratable acidity (TA), volatile flavor compounds, and sensory evaluation of overall flavor were studied., Taste panel tests indicated that the lower fertilization level (100 lb/A N- 150 lb/A K) yielded tomatoes with a more desirable flavor. Tomatoes grown at the two higher levels of fertilization (200 lb/A N-300 lb/A K; 300 lb/A N-450 lb/A K) did not differ in flavor. Soluble solids and TA were found to increase with increasing levels of N and K. There was a modest trend toward lower pH values in tomatoes grown at lower fertilization levels. Although no qualitative differences were observed among volatiles from the three treatments, several compounds were found to change quantitatively with treatment. MS and GC enrichment were used to tentatively identify these volatiles. Hexenal, 2-hexanone, 2,4-hexadienal, benzaldehyde, phenylacetaldehyde, beta-ionone and 6-methyl-5-hepten-2-one were found to increase with increasing levels of N and K fertilization. Farnesol isomers appeared maximally in treatment II and eugenol decreased with increasing fertilization., This study indicates that tomatoes treated with high levels of N and K do not exhibit desirable flavor despite an increase in both TA and soluble solids. This may be due to the intrusion of undesirable flavors from volatiles whose production is increased under conditions of high N and K fertilization.
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Date Issued
1982, 1982
Identifier
AAI8306177, 3085474, FSDT3085474, fsu:74966
Format
Document (PDF)
THE GEORGIA EXPANDED FOOD AND NUTRITION EDUCATION PROGRAM'S LONG-TERM EFFECTS ON DIETARY INTAKE AND FOOD BEHAVIOR PRACTICES
Title
THE GEORGIA EXPANDED FOOD AND NUTRITION EDUCATION PROGRAM'S LONG-TERM EFFECTS ON DIETARY INTAKE AND FOOD BEHAVIOR PRACTICES.
Creator
BROWN, AMANDA JONES., The Florida State University
Date Issued
1979, 1979
Identifier
AAI8001087, 2989310, FSDT2989310, fsu:73817
Format
Document (PDF)
PURIFICATION AND CHARACTERIZATION OF AN ANTIMICROBIAL SUBSTANCE PRODUCED BY LACTOBACILLUS BULGARICUS (LACTIC ACID BACTERIA, LACTOHACILLI, SUGAR EFFECT, HPLC, MASS SPECTROMETRY)
Title
PURIFICATION AND CHARACTERIZATION OF AN ANTIMICROBIAL SUBSTANCE PRODUCED BY LACTOBACILLUS BULGARICUS (LACTIC ACID BACTERIA, LACTOHACILLI, SUGAR EFFECT, HPLC, MASS SPECTROMETRY).
Creator
ABDEL-BAR, NADIA MOKHTAR., Florida State University
Abstract/Description
The present study was designed to achieve three goals: (1) to investigate some physical properties of the crude antimicrobial substance (lactobulgarican) produced by Lactobacillus bulgaricus, (2) to test the effect of several types of sugar present in the growth medium of L. bulgaricus on production of lactobulgarican, and (3) to purify and characterize the pure lactobulgarican., Lactobulgarican was located extracellularly and was more active toward gram-negative than -positive organisms. It...
Show moreThe present study was designed to achieve three goals: (1) to investigate some physical properties of the crude antimicrobial substance (lactobulgarican) produced by Lactobacillus bulgaricus, (2) to test the effect of several types of sugar present in the growth medium of L. bulgaricus on production of lactobulgarican, and (3) to purify and characterize the pure lactobulgarican., Lactobulgarican was located extracellularly and was more active toward gram-negative than -positive organisms. It had an optimum pH of 4.0. It was reversibly inactivated at pH range of 5.0-12.0 but irreversibly inactivated at pH 1.0. At the optimum pH, lactobulgarican was highly thermostable. It was also stable to refrigerated storage up to 18 days., Carbohydrate-free formula, with a given type of sugar added, was used to test the effect of sugar on lactobulgarican production. Among the sugars tested, glucose produced the lowest pH value and the largest inhibition zone under a short incubation period, i.e., 48-hours. Under a long incubation period, i.e., 96-hours, both glucose and lactose, at the same concentration, produced comparable results. A ninety-six hour incubation period was the best one among those tested. Fructose and sucrose were not fermented by L. bulgaricus, and did not produce any inhibition zones., Reversed phase HPLC column was used to purify lactobulgarican. Lactobulgarican did not contain lactic acid or hydrogen peroxide. The UV spectrum of lactobulgarican produced two broad peaks at 198 and 274 nm. Negative and positive chemical ionization mass spectra produced peaks at m/e 181, 180, 163 and 179, 91, 88, 57, respectively. The biuret test for lactobulgarican was negative. Results from the UV and mass spectra indicated the possible presence of a hexose and a phenol-containing compound, possibly phenylalanine. The molecular weight of lactobulgarican was (LESSTHEQ) 507.
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Date Issued
1984, 1984
Identifier
AAI8505280, 3086011, FSDT3086011, fsu:75497
Format
Document (PDF)
Walnut (Juglans regia L.) characterization: Protein composition, lipid and tannin content, and sensory quality
Title
Walnut (Juglans regia L.) characterization: Protein composition, lipid and tannin content, and sensory quality.
Creator
Sze-Tao, Kar Wai Clara., Florida State University
Abstract/Description
Walnuts contained about 16.66% protein and 66.90% lipids. Linoleic (61.21%) and linolenic (13.81%) acids accounted for up to 75% of the total fatty acids in walnut lipids. The majority of walnut proteins were soluble in 0.1 M NaOH with a minimum solubility at pH 4. Albumins, globulins, prolamins, and glutelins respectively accounted for 6.81, 17.57, 5.33, and 70.11% of the total protein. Both protein solubility and electrophoretic analyses revealed glutelins to be the major walnut proteins....
Show moreWalnuts contained about 16.66% protein and 66.90% lipids. Linoleic (61.21%) and linolenic (13.81%) acids accounted for up to 75% of the total fatty acids in walnut lipids. The majority of walnut proteins were soluble in 0.1 M NaOH with a minimum solubility at pH 4. Albumins, globulins, prolamins, and glutelins respectively accounted for 6.81, 17.57, 5.33, and 70.11% of the total protein. Both protein solubility and electrophoretic analyses revealed glutelins to be the major walnut proteins. The total protein contained at least seven major polypeptides with estimated molecular weight range 19,590-21,830 and 33,340-37,150. Lysine was the first limiting essential amino acid in total proteins, globulins, and glutelins. Leucine and methionine plus cysteine were the limiting amino acids, respectively, for prolamins and albumins. Hydrophobic and acidic amino acids dominated the amino acid composition in all protein fractions. Minimum extractability of nitrogen at 0.5-1 M TCA represented the nonprotein nitrogen compounds in defatted walnuts. The nonprotein nitrogen values in walnuts ranged from 6.24 to 6.50% of the total nitrogen. Native or heat denatured walnut glutelins were easily hydrolyzed in vitro by the common digestive proteases. The tannin content of inshell and shelled walnuts was in the range of 889-1095 and 574.4-667.2 mg catechin equiv./100 g walnut, respectively. After three weeks of storage, walnut assayable tannins were reduced by about 20%. Blanched walnuts (BW) retained about 2% of assayable tannins, had a buttery-sweet taste, and rated less astringent then unblanched walnuts (UW). The astringency scores and tannin content of BW were correlated (r = 0.9249).
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Date Issued
1996, 1996
Identifier
AAI9622870, 3088887, FSDT3088887, fsu:77686
Format
Document (PDF)
A molecular and cytogenetic analysis of asymmetric somatic hybrids of the genus Nicotiana
Title
A molecular and cytogenetic analysis of asymmetric somatic hybrids of the genus Nicotiana.
Creator
Piastuch, William Craig., Florida State University
Abstract/Description
A molecular and cytogenetic characterization of asymmetric somatic hybrids of Nicotiana was performed to study the use of somatic hybridization as a tool for gene transfer between plant species. The asymmetric somatic hybrids had been previously formed by protoplast fusion between wild-type Nicotiana tabacum and a plant line of N. plumbaginifolia carrying a stably integrated kanamycin-resistance gene. These plants were examined with respect to their morphology, fertility, organellar and...
Show moreA molecular and cytogenetic characterization of asymmetric somatic hybrids of Nicotiana was performed to study the use of somatic hybridization as a tool for gene transfer between plant species. The asymmetric somatic hybrids had been previously formed by protoplast fusion between wild-type Nicotiana tabacum and a plant line of N. plumbaginifolia carrying a stably integrated kanamycin-resistance gene. These plants were examined with respect to their morphology, fertility, organellar and nuclear genomic organization, and inheritance of the selectable marker. To determine the extent of genomic asymmetry in the hybrids, a species-specific repetitive DNA sequence was cloned from N. plumbaginifolia and used as a probe for both dot blot and in situ hybridization studies. Dot-blot hybridization data indicated two groups of hybrid plants: Asymmetric hybrids containing 15-25% N. plumbaginifolia DNA and extremely asymmetric hybrids with 1-4% N. plumbaginifolia DNA. Using in situ hybridization it was possible to visualize individual N. plumbaginifolia chromosomes in the hybrids. The asymmetric hybrids retain 8-12 chromosomes and chromosome fragments from N. plumbaginifolia, whereas the extremely asymmetric hybrids contain only a single N. plumbaginifolia chromosome. In several of the hybrids it was possible to show the presence of intergenomic translocations between the chromosomes of N. tabacum and N. plumbaginifolia. RFLP analysis, using a cloned 5S-gene repeat sequence, provided evidence for chromosomal linkage of the kanamycin-resistance gene and a 5S-gene cluster from N. plumbaginifolia. Absence of this linkage was correlated with intergenomic translocations in two of the hybrids. Analysis of organellar DNAs was performed to detect any extra-nuclear genomic recombination in the hybrids. The results of these analyses were correlated with morphological and, fertility data in an attempt to understand the molecular basis of male sterility and partial female fertility exhibited in the asymmetric hybrids.
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Date Issued
1990, 1990
Identifier
AAI9118496, 3162235, FSDT3162235, fsu:78433
Format
Document (PDF)
PROTON INDUCED X-RAY EMISSION ANALYSIS FOR THE DETERMINATION OF NUTRITIONAL STATUS IN PRESCHOOL CHILDREN
Title
PROTON INDUCED X-RAY EMISSION ANALYSIS FOR THE DETERMINATION OF NUTRITIONAL STATUS IN PRESCHOOL CHILDREN.
Creator
HENLEY, EDNA CHESTINE., The Florida State University
Date Issued
1976, 1976
Identifier
AAI7629448, 2988380, FSDT2988380, fsu:72887
Format
Document (PDF)
THE EFFECTS OF SERVING STYLE ON FOOD WASTE IN A SCHOOL LUNCH PROGRAM
Title
THE EFFECTS OF SERVING STYLE ON FOOD WASTE IN A SCHOOL LUNCH PROGRAM.
Creator
HEIMBERG, JUDITH STANLEY., The Florida State University
Date Issued
1978, 1978
Identifier
AAI7909764, 2989037, FSDT2989037, fsu:73544
Format
Document (PDF)
VEGETABLE EXTRACTS AS ANTIBACTERIAL PRESERVATIVES AND THEIR APPLICATION IN FOODS
Title
VEGETABLE EXTRACTS AS ANTIBACTERIAL PRESERVATIVES AND THEIR APPLICATION IN FOODS.
Creator
WOO, HAYMIE CHOI., The Florida State University
Date Issued
1973, 1973
Identifier
AAI7408419, 2987498, FSDT2987498, fsu:72007
Format
Document (PDF)
STUDIES ON MALONALDEHYDE IN LIPID OXIDATION
Title
STUDIES ON MALONALDEHYDE IN LIPID OXIDATION.
Creator
KWON, TAI-WAM., The Florida State University
Date Issued
1963, 1963
Identifier
AAI6403599, 2985167, FSDT2985167, fsu:69677
Format
Document (PDF)
THE ROLE OF PYRIDINE NUCLEOTIDES, SUBSTRATES, AND INTERMEDIATES IN THE ENZYMATIC REDUCTION OF METMYOGLOBIN IN GROUND BEEF
Title
THE ROLE OF PYRIDINE NUCLEOTIDES, SUBSTRATES, AND INTERMEDIATES IN THE ENZYMATIC REDUCTION OF METMYOGLOBIN IN GROUND BEEF.
Creator
SALEH, BASSIMA ABDEL-HAMID., The Florida State University
Date Issued
1967, 1967
Identifier
AAI6711168, 2985631, FSDT2985631, fsu:70140
Format
Document (PDF)
ANTIOXIDANT ACTIVITY OF VEGETABLE FLAVONOIDS IN LIPID-AQUEOUS SYSTEMS
Title
ANTIOXIDANT ACTIVITY OF VEGETABLE FLAVONOIDS IN LIPID-AQUEOUS SYSTEMS.
Creator
PRATT, DAN EDWARD., The Florida State University
Date Issued
1963, 1963
Identifier
AAI6403609, 2985188, FSDT2985188, fsu:69699
Format
Document (PDF)
LIPID OXIDATION AND PIGMENT CHANGES IN FRESH AND IRRADIATED BEEF
Title
LIPID OXIDATION AND PIGMENT CHANGES IN FRESH AND IRRADIATED BEEF.
Creator
GREENE, BARBARA ESTELLA., The Florida State University
Date Issued
1966, 1966
Identifier
AAI6706465, 2985588, FSDT2985588, fsu:70097
Format
Document (PDF)
DETERIORATIVE CHANGES IN FROZEN SHRIMP AND THEIR INHIBITION
Title
DETERIORATIVE CHANGES IN FROZEN SHRIMP AND THEIR INHIBITION.
Creator
CHASTAIN, MARIAN B. FAULKNER., The Florida State University
Date Issued
1955, 1955
Identifier
AAI5902976, 2984921, FSDT2984921, fsu:69377
Format
Document (PDF)
HEAT INDUCED HEME CATALYZED LIPID OXIDATION IN ANIMAL TISSUES
Title
HEAT INDUCED HEME CATALYZED LIPID OXIDATION IN ANIMAL TISSUES.
Creator
TARLADGIS, BASIL GEORGE., The Florida State University
Date Issued
1960, 1960
Identifier
AAI6003317, 2984933, FSDT2984933, fsu:69391
Format
Document (PDF)
ANTIOXIDANT ACTIVITY OF VEGETABLE EXTRACTS
Title
ANTIOXIDANT ACTIVITY OF VEGETABLE EXTRACTS.
Creator
COFER, AGNES CHAMBLESS., The Florida State University
Date Issued
1964, 1964
Identifier
AAI6500296, 2985239, FSDT2985239, fsu:69750
Format
Document (PDF)
LIPOXIDASE AND LIPID OXIDATION IN VEGETABLES
Title
LIPOXIDASE AND LIPID OXIDATION IN VEGETABLES.
Creator
RHEE, KISOON (CHOI)., The Florida State University
Date Issued
1965, 1965
Identifier
AAI6515483, 2985393, FSDT2985393, fsu:69902
Format
Document (PDF)
INVESTIGATIONS ON THE PRESERVATION OF OYSTERS
Title
INVESTIGATIONS ON THE PRESERVATION OF OYSTERS.
Creator
SCHWARTZ, MARK G., The Florida State University
Date Issued
1959, 1959
Identifier
AAI5901764, 2984849, FSDT2984849, fsu:69291
Format
Document (PDF)
RELATIONSHIP OF HEME PIGMENTS TO LIPID OXIDATION IN ANIMAL TISSUES
Title
RELATIONSHIP OF HEME PIGMENTS TO LIPID OXIDATION IN ANIMAL TISSUES.
Creator
ZIPSER, MARELYNN WEISS., The Florida State University
Date Issued
1963, 1963
Identifier
AAI6407590, 2985212, FSDT2985212, fsu:69723
Format
Document (PDF)
OXIDATION OF ASCORBIC ACID IN COPPER-CATALYZED SUCROSE SOLUTIONS
Title
OXIDATION OF ASCORBIC ACID IN COPPER-CATALYZED SUCROSE SOLUTIONS.
Creator
HSIEH, YUN-HWA PEGGY., Florida State University
Abstract/Description
The purposes of the present study were: to investigate the influence of oxygen, copper (Cu) concentration, buffer, pH and temperature on the rate of oxidation of ascorbic acid (AA) in sucrose solutions; and to define the physical and chemical roles of sucrose on AA stability. Investigations were conducted both in opened and closed systems. In the opened system the oxygen supply was manipulated by the surface to volume ratio of sample plus different speed of shaking. The parameter monitored...
Show moreThe purposes of the present study were: to investigate the influence of oxygen, copper (Cu) concentration, buffer, pH and temperature on the rate of oxidation of ascorbic acid (AA) in sucrose solutions; and to define the physical and chemical roles of sucrose on AA stability. Investigations were conducted both in opened and closed systems. In the opened system the oxygen supply was manipulated by the surface to volume ratio of sample plus different speed of shaking. The parameter monitored was residual AA determined by titration with 2,6-dicholoro-phenolindophenol. In the closed system the oxygen uptake kinetics were studied at different temperature (26.5(DEGREES)C, 30(DEGREES)C, and 33(DEGREES)C) and pH levels (3.2, 4.25 and 5.0). Cupric ion activity of added copper in sucrose solutions was measured using a cupric electrode., The results of the first system showed that if the viscosity effect could be eliminated, sucrose actually enhanced the oxidation of AA, especially in the Cu-catalyzed reactions. Cupric ion activity was greater in sucrose solutions than in the absence of sucrose. The destructive effect of sucrose on AA in Cu-catalyzed reactions was mainly due to the increased activity of Cu in sucrose solutions. The destructive effect of sucrose was lower in acetate buffered solutions than in unbuffered solutions at the same pH level (3.2) due to the Cu-complexing power of buffer salts. Observations from the second system generally agree with that of the first one. The pseudo-first order and second-order rate constants of AA oxidation in various solutions are presented and activation energies were calculated by Arrhenius equation. Both in Cu-catalyzed and non-catalyzed oxidation of AA, Approximately one mole of oxygen was used per mole of AA.
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Date Issued
1987, 1987
Identifier
AAI8713319, 3086615, FSDT3086615, fsu:76090
Format
Document (PDF)
ANTIOXIDANT EFFECT OF NON-ENZYMATIC BROWNING REACTION PRODUCTS ON LINOLEIC ACID
Title
ANTIOXIDANT EFFECT OF NON-ENZYMATIC BROWNING REACTION PRODUCTS ON LINOLEIC ACID.
Creator
KIM, NAN-SOOK KOO., Florida State University
Abstract/Description
Oxidation products of unsaturated fatty acids in foods cause rancid flavor and limit the storage stability of food products. Even though many synthetic antioxidants are used, increasing interest has been directed towards the utilization of normal food constituents having antioxidative properties., In aqueous lysine-sucrose model systems, the effects of reaction time, pH and molar ratio were studied on the formation of non-enzymatic browning reaction (NBR) products. The extent of sucrose...
Show moreOxidation products of unsaturated fatty acids in foods cause rancid flavor and limit the storage stability of food products. Even though many synthetic antioxidants are used, increasing interest has been directed towards the utilization of normal food constituents having antioxidative properties., In aqueous lysine-sucrose model systems, the effects of reaction time, pH and molar ratio were studied on the formation of non-enzymatic browning reaction (NBR) products. The extent of sucrose hydrolysis was measured and the antioxidant effect of NBR products on linoleic acid emulsions was examined. Nuclear magnetic resonance (NMR) spectra were obtained at various stages of browning., Sucrose produced NMR products with lysine in an aqueous system at 100(DEGREES)C. The C-13 NMR spectra indicated that the NBR started when sucrose and lysine were dissolved in water. The C-13 NMR spectra also showed that heating did not have an effect on the gross composition of polymeric species. The absorbance at 480 nm of 0.75 M lysine-sucrose solution heated up to 6 hours increased with reaction times. The pH values of heated lysine-sucrose solution gradually decreased with reaction time. When 0.75 M sucrose or lysine solution was heated separately up to 6 hours, there were no changes in absorbance in pH. In the pH range of 3.52 - 6.35, higher absorbance was obtained from heated lysine-sucrose solution at acidic pH levels than at neutral pH levels., As the concentration of lysine and sucrose solution was increased, the absorbance increased. At longer reaction times or acidic pH levels, enhanced hydrolysis of sucrose to reducing sugars resulted in more NBR products. NBR products obtained at a longer reaction time, an acidic pH, and higher concentration of reactants showed a darker brown color and were effective in preventing the formation of peroxides. The oxygen uptake of linoleic acid emulsion having NBR products was smaller than that of linoleic acid emulsion without NBR products. Based on these results, it was concluded that sucrose may act as an antioxidant in processed foods containing both amino acids and lipids.
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Date Issued
1987, 1987
Identifier
AAI8711725, 3086576, FSDT3086576, fsu:76051
Format
Document (PDF)
Floating Islands brand Florida vegetables label
Title
Floating Islands brand Florida vegetables label.
Creator
Floating Islands Brand, W.E. Christian Company
Abstract/Description
Label for the Floating Islands brand Florida vegetables by W.E. Christian Company of McIntosh, Florida.
Date Issued
1880, 1920
Identifier
FSU_MSS_1967_113_B146_F001_I003
Format
Image (JPEG2000)
Tallahassee Lake Elberta FAMU
Title
Tallahassee Lake Elberta FAMU.
Creator
Florida State University, Gross, Barry
Date Issued
2008
Identifier
FSU_HUA_2018_043_0128
Format
Image (JPEG2000)
FAMU Southwest Campus
Title
FAMU Southwest Campus.
Creator
Florida State University, Gross, Barry
Date Issued
2007
Identifier
FSU_HUA_2018_043_0058
Format
Image (JPEG2000)
Lake Elberta FAMU Lake Bradford Complex
Title
Lake Elberta FAMU Lake Bradford Complex.
Creator
Florida State University, Gross, Barry
Date Issued
2007
Identifier
FSU_HUA_2018_043_0063
Format
Image (JPEG2000)
Tallahassee Lake Elberta FAMU
Title
Tallahassee Lake Elberta FAMU.
Creator
Florida State University, Gross, Barry
Date Issued
2008
Identifier
FSU_HUA_2018_043_0143
Format
Image (JPEG2000)
AN IN VITRO SYSTEM FOR STUDIES OF DEVELOPMENTAL CONTROL OF GENE EXPRESSION IN SEA URCHINS
Title
AN IN VITRO SYSTEM FOR STUDIES OF DEVELOPMENTAL CONTROL OF GENE EXPRESSION IN SEA URCHINS.
Creator
MORRIS, GILBERT FRANKLIN., Florida State University
Abstract/Description
The synthesis of RNA in isolated nuclei from two sea urchin species was studied. Isolated nuclei from sea urchin embryos synthesize a variety of RNAs in vitro. Two small RNAs, 5S rRNA and N1 RNA, made in isolated nuclei are identical to the small RNAs detected in vivo by hybridization selection with specific cloned sea urchin DNAs. RNAs complementary to DNA clones for histone mRNAs and rRNAs were also detected among the in vitro transcripts. The rRNAs appear to initiate and terminate...
Show moreThe synthesis of RNA in isolated nuclei from two sea urchin species was studied. Isolated nuclei from sea urchin embryos synthesize a variety of RNAs in vitro. Two small RNAs, 5S rRNA and N1 RNA, made in isolated nuclei are identical to the small RNAs detected in vivo by hybridization selection with specific cloned sea urchin DNAs. RNAs complementary to DNA clones for histone mRNAs and rRNAs were also detected among the in vitro transcripts. The rRNAs appear to initiate and terminate transcription in the isolated nuclei near the correct DNA sequences. Synthesis of these four RNAs faithfully in the isolated nuclei encompasses the activities of all three RNA polymerases., The addition of cell extracts modifies the activity of the RNA polymerases in the isolated embryo nuclei. Extracts from sea urchin eggs specifically inhibit RNA polymerase III activity in the isolated nuclei. This activity was purified from the crude egg extracts approximately fifty fold. The inhibition of RNA polymerase III does not appear to be due to a nonspecific activity in the egg extract. The partially purified inhibitor from the egg extract also inhibits RNA polymerase III in DNA dependent transcription assays from mouse and Drosophila. The inhibitor probably interferes with RNA polymerase III initiation by an unknown triphosphate dependent mechanism., The activity of the inhibitor is destroyed within thirty minutes of fertilization. The rapid destruction of the inhibitor activity by fertilization suggests that this activity is biologically relevant. The inactivation of the inhibitor may be at least partially due to the ionic changes associated with fertilization. The activity of the inhibitor may be related to a 135,000 molecular weight protein which can be detected in preparations from eggs but not in parallel preparations from embryos which lack the inhibitor., This dissertation demonstrates in an in vitro assay a possible control mechanism for RNA polymerase III activity in sea urchin development. It is conceivable that such a mechanism is operative in other organisms as well.
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Date Issued
1982, 1982
Identifier
AAI8304078, 3085443, FSDT3085443, fsu:74936
Format
Document (PDF)
COMPARATIVE FUNCTIONAL MORPHOLOGY OF THE FEEDING APPENDAGES AND ASSOCIATED STRUCTURES OF THE TROPICAL FRESHWATER SHRIMPS ATYA INNOCOUS (HERBST) AND POTIMIRIM GLABRA (KINGSLEY)
Title
COMPARATIVE FUNCTIONAL MORPHOLOGY OF THE FEEDING APPENDAGES AND ASSOCIATED STRUCTURES OF THE TROPICAL FRESHWATER SHRIMPS ATYA INNOCOUS (HERBST) AND POTIMIRIM GLABRA (KINGSLEY).
Creator
FELGENHAUER, BRUCE EUGENE., Florida State University
Abstract/Description
Atya innocous occurs in permanent and temporary freshwater streams in Panama. Its microhabitat distribution depends in part on the presence of congeners. The propodus and dactylus of each cheliped of A. innocous bear about 300 setae modified for specific functions in food collection. These setae may be divided into three basic types: chemoreceptors, scrapers, and filtering setae. The latter two types also function as mechanoreceptors. The cheliped setae are probably extended by hemocoelic...
Show moreAtya innocous occurs in permanent and temporary freshwater streams in Panama. Its microhabitat distribution depends in part on the presence of congeners. The propodus and dactylus of each cheliped of A. innocous bear about 300 setae modified for specific functions in food collection. These setae may be divided into three basic types: chemoreceptors, scrapers, and filtering setae. The latter two types also function as mechanoreceptors. The cheliped setae are probably extended by hemocoelic pressure, producing an efficient filtering fan. Mechanosensory and chemosensory bipolar neurons innervate the cheliped setae, presumably providing the shrimp with the ability to detect current velocity, particulate load and food sources. The external morphology and ultrastructure of these sensilla are described., The peropods are also used to groom body parts and brooded embryos. Super-8 cine films show the role mouthparts play in grooming. The third maxillipeds groom the antennae and antennules of the prawn, with the distal endite of the second maxillipeds freeing lodged debris from pereopods one and two. The pectinate comb of the fifth pereopod grooms the dorsal surface of the abdomen and pleopods., Electron microscopy showed pores leading to unicellular glands within the epidermis. Three gland types were observed and their respective functions described., Adaptations for a lotic habitat seem to have dominated atyid evolution. With the exception of lake species, most atyid shrimp have a wide array of morphological modifications for life in running water., The mouthparts, mandibles and foregut of Atya innocous and another atyid Potimirim glabra are compared to test the hypothesis that these structures are related to diet. Potimirim glabra inhabits quiet pools scraping periphyton from leaf surfaces and decaying organic detritus. The feeding appendages of both species are modified for handling fine food. The mandible of these shirmp have a strong incisor and molar process but are not utilized for crushing large food items. The primitive nature of the mandibles and foregut suggest the placement of the family Atyidae at the base of the caridean evolutionary tree.
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Date Issued
1982, 1982
Identifier
AAI8309271, 3085524, FSDT3085524, fsu:75016
Format
Document (PDF)
Small farmers in three Florida counties: An exploratory study of some factors affecting the utilization of 1890 Land-Grant University Cooperative Extension Services
Title
Small farmers in three Florida counties: An exploratory study of some factors affecting the utilization of 1890 Land-Grant University Cooperative Extension Services.
Creator
Viegbesie, Anthony O., Florida State University
Abstract/Description
The purpose of this study was to examine small farmers in three North Florida counties (Gadsden, Jackson and Jefferson) to ascertain the factors determining the utilization of the 1890 Land-Grant University Cooperative Extension Services. Specifically, the study examines the social and economic characteristics of the small farmer utilizing the Cooperative Extension Services and the small farmer's perceptions of how much the Cooperative Extension Services are satisfying their farm and family...
Show moreThe purpose of this study was to examine small farmers in three North Florida counties (Gadsden, Jackson and Jefferson) to ascertain the factors determining the utilization of the 1890 Land-Grant University Cooperative Extension Services. Specifically, the study examines the social and economic characteristics of the small farmer utilizing the Cooperative Extension Services and the small farmer's perceptions of how much the Cooperative Extension Services are satisfying their farm and family needs., To obtain information for the study, a questionnaire was administered to survey small farmers in Gadsden, Jackson and Jefferson counties. Descriptive statistics, especially frequencies, percentages, mean and mode were the basic statistical technique used to analyze collected data. Findings were summarized in tables., The major findings of this research helped ascertain which specific factors were associated with small farmers' utilization of specific Cooperative Extension Service programs. It may also serve as a stimulus for further research on Cooperative Extension Service programs.
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Date Issued
1989, 1989
Identifier
AAI8915770, 3161778, FSDT3161778, fsu:77977
Format
Document (PDF)
Studies of arginine kinase in the horseshoe crab, Limulus polyphemus, with regard to potential intracellular compartmentation
Title
Studies of arginine kinase in the horseshoe crab, Limulus polyphemus, with regard to potential intracellular compartmentation.
Creator
Doumen, Chris., Florida State University
Abstract/Description
The research outlined in this dissertation represents a study towards the examination of the presence of mitochondrial arginine kinase (AK) activity in horseshoe crab (Limulus polyphemus) hearts and its possible physiological significance. The existence of such a mitochondrial bound AK was hinted at by the residual enzymatic activity in the mitochondrial pellet and the ability of arginine to stimulate state 3 respiration in the presence of ATP. The presence of a mitochondrial bound AK (AKm)...
Show moreThe research outlined in this dissertation represents a study towards the examination of the presence of mitochondrial arginine kinase (AK) activity in horseshoe crab (Limulus polyphemus) hearts and its possible physiological significance. The existence of such a mitochondrial bound AK was hinted at by the residual enzymatic activity in the mitochondrial pellet and the ability of arginine to stimulate state 3 respiration in the presence of ATP. The presence of a mitochondrial bound AK (AKm) in L. polyphemus hearts was confirmed by sucrose-density centrifugation and solubilization experiments. Between 1.5 and 2% of total cytoplasmic AK activity appeared to be associated with the mitochondria. Cellulose acetate electrophoresis revealed the existence of two populations of AKm--a 'fast' electrophoretic form (AKmf), migrating identically to cytoplasmic AK (AKc), and a 'slow' electrophoretic form (AKms). Both forms were present in a 1:1 ratio. The 'fast' form was easily extractable while AKms showed hydrophobic characteristics, only extractable with detergents. SDS-PAGE electrophoresis of these isoenzymes following purification showed the molecular weight subunit of both AKc and AKmf to be virtually the same (39-40 kD), but that of AKms being slightly higher (43.5 kD). The peptide maps of AKc and AKmf were identical, whereas that of AKms displayed some unique peptide fragments. AKmf is therefore thought to be a similar protein as AKc while AKmf may contain, however, an additional hydrophobic sequence for insertion and anchoring into the inner membrane of the mitochondrion., Kinetic and thermodynamic experiments in the presence and absence of oxidative phosphorylation (OP) showed no difference in the kinetic parameters or movement of the mass action ratio, respectively. These results thus do not suggest effective functional coupling between OP (e.g. the adenosine nucleotide translocator) and the AKm reaction. These experiments exclude the possibility that there is preferential access of the AKm enzyme(s) for the product of respiration (ATP). A study comparing the efficacy of bound mitochondrial AK versus a soluble source of ADP showed that mitochondrial associated AK might be advantageous by minimizing the diffusion of ADP.
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Date Issued
1990, 1990
Identifier
AAI9024093, 3162014, FSDT3162014, fsu:78212
Format
Document (PDF)
The structure and the expression of sea urchin U1 gene
Title
The structure and the expression of sea urchin U1 gene.
Creator
Yu, Jin-Chen., Florida State University
Abstract/Description
Sea urchin U1 genes coding for the major embryonic U1 RNA are in the tandemly repeated units. The U1 repeat unit in L. variegatus is variable in size from 1.2 to 1.4 Kb while that in S. purpuratus has a homogenous size of 1.1 Kb. Two types of U1 repeats have been isolated from L. variegatus. They are conserved in the 5$\sp\prime$ flanking region for 500 bp, but vary in the 3$\sp\prime$ flanking sequence immediately after the CAAAGAAAGAAAA sequence necessary for 3$\sp\prime$ end formation. The...
Show moreSea urchin U1 genes coding for the major embryonic U1 RNA are in the tandemly repeated units. The U1 repeat unit in L. variegatus is variable in size from 1.2 to 1.4 Kb while that in S. purpuratus has a homogenous size of 1.1 Kb. Two types of U1 repeats have been isolated from L. variegatus. They are conserved in the 5$\sp\prime$ flanking region for 500 bp, but vary in the 3$\sp\prime$ flanking sequence immediately after the CAAAGAAAGAAAA sequence necessary for 3$\sp\prime$ end formation. The tandemly repeated U1 cluster of S. purpuratus ends just 3$\sp\prime$ to the U1 coding region., The sea urchin U1 gene is transcribed by RNA polymerase II in isolated nuclei, but it can be transcribed by RNA polymerase III in sea urchin nuclear extracts. Two assays were used for testing promoter function, a microinjection assay for testing the expression of U1 genes in vivo and a DNA-dependent nuclear extract for testing expression in vitro. At least 200 nucleotides, but no more than 345 nucleotides, 5$\sp\prime$ to the gene are required for the expression of U1 gene in vivo. Two of the four highly conserved regions between L. variegatus and S. purpuratus U1 5$\sp\prime$ flanking region are in these 142 nucleotides. In contrast, none of the 5$\sp\prime$ flanking region, but rather an internal promoter region is required for the synthesis of U1 RNA by RNA polymerase III in vitro. The internal promoter region contains a tRNA A-box consensus sequence and an 11-bp region with identical sequence to one of the highly conserved regions. These data suggest that the four highly conserved regions in the 5$\sp\prime$ flanking sequence are important for the expression of the sea urchin U1 gene.
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Date Issued
1989, 1989
Identifier
AAI9012942, 3161939, FSDT3161939, fsu:78138
Format
Document (PDF)
ECOLOGICAL INVESTIGATIONS OF THE EPIFAUNA AND FLORA OF BAY SCALLOPS, WITH SPECIAL REFERENCE TO FREE-LIVING NEMATODES
Title
ECOLOGICAL INVESTIGATIONS OF THE EPIFAUNA AND FLORA OF BAY SCALLOPS, WITH SPECIAL REFERENCE TO FREE-LIVING NEMATODES.
Creator
SHERMAN, KEVIN MICHAEL., Florida State University
Abstract/Description
The fauna and flora living on the bay scallop, Argopecten irradians, was examined from twenty-five consecutive monthly collections. The nematode fauna of scallops differed from that of nearby sediments and seagrasses in that it was less diverse and was dominated by three species, Viscosia macramphida, Syringolaimus striatocaudatus, and Chromadora nudicapitata. These species were rare in sediment samples and common, though not as abundant, on seagrasses. They accounted for 79.3% of the total...
Show moreThe fauna and flora living on the bay scallop, Argopecten irradians, was examined from twenty-five consecutive monthly collections. The nematode fauna of scallops differed from that of nearby sediments and seagrasses in that it was less diverse and was dominated by three species, Viscosia macramphida, Syringolaimus striatocaudatus, and Chromadora nudicapitata. These species were rare in sediment samples and common, though not as abundant, on seagrasses. They accounted for 79.3% of the total individuals on scallops. The simplicity of this nematode assemblage was exploited in field and laboratory experiments designed to uncover the ecological factors responsible for the temporal variation of the dominant species., Initial field experiments justified the view of scallop shells as isolated communities because the swimming activities of scallops did not remove the shell's nematode fauna, and space experimentally made available on a scallop shell was not appreciably recolonized over a four-hour period. Consequently, the system could be studied experimentally., The major finding of the research was discovering that each species responded to a different ecological factor. Viscosia was most abundant on scallop shells in late summer. Its abundance coincided with a peak in diatom abundance, its preferred food. Chromadora was abundant in the spring on young scallops that had recently detached from blades of the seagrass Thalassia testudinum. At that time, Chromadora was the most abundant nematode on the blades, so its abundance on newly steeled scallops was a reflection of the recent history of the shell. It remained abundant on scallops until the early summer. A long-term field recolonization experiment determined that immigration of Chromadora from seagrasses was not responsible for the continued success of the shell populations. Syringolaimus increased in abundance over the course of the study, and was the overall dominant on scallop shells once Chromadora populations declined. A laboratory culture experiment determined that Syringolaimus was not able to competitively exclude Chromadora under conditions approximating those in the field. Rather, the species coexisted in densities consistent with their field values.
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Date Issued
1985, 1985
Identifier
AAI8607381, 3086297, FSDT3086297, fsu:75780
Format
Document (PDF)

Pages