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Sister chromatids attach to microtubules originating from opposite spindle poles to establish bipolar attachment, which generates tension on chromosomes and is essential for accurate chromosome segregation. Incorrect chromosome attachment or lack of tension results in activation of the Spindle Assembly Checkpoint (SAC) that prevents anaphase onset until correct chromosome attachment is achieved. Cin8, a microtubule motor protein, is recruited to the spindle midzone and kinetochores during metaphase and may play an important role in tension generation and SAC silencing. Another kinesin-5 motor protein Kip1 plays a redundant function with Cin8. To study the function of Cin8 in chromosome attachment, we constructed an auxin-inducible degron (AID) mutant CIN8-AID. With this conditional mutant, we first found that CIN8-AID kip1∆ grew poorly in the presence of auxin, which induces Cin8-AID degradation, confirming the synthetic lethality. We further found that the absence of either SAC or tension checkpoint abolishes the cell cycle delay in cin8 mutant. These results suggest the function of Cin8 in chromosome bipolar attachment and tension generation.