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Detrimental Effects of Diet-Induced Obesity on Olfactory Performance and Function

Title: The Detrimental Effects of Diet-Induced Obesity on Olfactory Performance and Function.
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Name(s): Schreiter, Nicholas Alexander, author
Type of Resource: text
Genre: Text
Date Issued: 2016-12-08
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Diet-induced obesity (DIO) decreases the number of olfactory sensory neurons (OSNs) in the nose and reduces their projections to the olfactory bulb. Whether surviving OSNs have equivalent odor responsivity is unknown. We utilized c-fos immediate early gene expression as a marker for neuronal activation to determine if DIO affects sensory function. Male mice that contained a gfp reporter for the M72 odorant receptor were challenged with control food (CF), moderately-high fat (MHF) diet, or high fat (HF) diet for 6 months upon weaning. Mice maintained on the modified diets weighed significantly more (one-way ANOVA, p<0.0001) and had reduced glucose clearance as determined by an intraperitoneal glucose tolerance test (one-way ANOVA, p<0.0001). Mice were exposed to isopropyl tiglate (IT) or clean air using a cyclic odor stimulation paradigm to activate the M72 odorant receptor. Immunocytochemical procedures demonstrated that the number of periglomerular (PG) cells did not decrease with fatty diets surrounding either the medial or lateral M72 glomerulus (one-way ANOVA; lateral (lat) – F(2, 11) = 0.4155, p = 0.67; medial (med) – F(2, 13) = 0.7149, p=0.5295). The M72 glomerular volume was also unaffected following DIO, which is consistent with PG cell abundance (one-way ANOVA; lat – F(2, 11) = 0.09720, p = 0.9081; med – F(2, 13) = 0.3194, p = 0.7321). Interestingly, unlike OSNs, PG cells do not show a reduction in abundance following DIO, which may suggest that PG cells possess resistance to the apoptotic effects of DIO via uninterrupted PG cell neurogenesis or other neuroprotective factors. The increase in c-fos immediate early gene activation with odor stimulation for PG cells surrounding the lateral and medial M72 glomerulus was not significantly different across dietary treatment (lat – Kruskal-Wallis = 2.367; p=0.3063; med – Kruskal-Wallis = 1.565; p = 0.4572). However, the mean fold change in c-fos positive PG cells revealed that the medial M72 glomerulus had decreased odor activation following DIO (CF = 5.45x, MHF = 2.49x, HF = 1.65x), while the lateral glomerulus had less decrease due to diet (CF = 2.95x, MHF = 1.95x, HF = 2.18x). This suggests that surviving OSNs projecting to the medial M72 glomerulus trend to have reduced activation by their ligand, IT, following DIO, but those projecting to the lateral glomerulus have similar odor sensitivity as CF mice. This work was supported by the Phi Eta Sigma Undergraduate Research Award, T32 DC000044, and R01 DC013080 from the National Institutes of Health (NIH) at the NIDCD.
Identifier: FSU_libsubv1_scholarship_submission_1481213576 (IID)
Keywords: Obesity, Diet-induced obesity, Olfaction, Periglomerular cells, Olfactory sensory neurons, Alzheimer’s Disease
Grant Number: T32 DC000044 and R01 DC013080 from the National Institutes of Health (NIH) at the NIDCD
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1481213576
Host Institution: FSU

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Schreiter, N. A. (2016). The Detrimental Effects of Diet-Induced Obesity on Olfactory Performance and Function. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1481213576