Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
Trace metals are important compounds in aquatic environments. In low concentrations, they can be very beneficial to aquatic life. However, they can be toxic if present in moderate to high concentrations. A growing interest has occurred involving ligands. Trace metals like copper are most commonly found complexed with ligands in fresh and marine waters. Many organisms also produce compounds to help mitigate increases in dissolved copper and prevent toxic environments. However, very little is known on how these ligands behave and what causes them to complex with trace metals. This project aims to separate and characterize copper binding ligands from a local lake in Tallahassee, FL. The motivation is to improve understanding of this complex process and mitigate future environmental issues dealing with elevated issues of metals in aquatic environments. UV/Vis spectrometry data did not yield any conclusive results since the peaks seen were very similar to what copper and the chelating agent used, EDTA, looked like. However, the data produced by the electrospray ionization mass spectrometer does indicate the presence of specific compounds which were affected by the pH of the solution. Although the data did not yield any characteristic data, we concluded that the IMAC process is capable of fractionating copper binding ligands.