Some of the material in is restricted to members of the community. By logging in, you may be able to gain additional access to certain collections or items. If you have questions about access or logging in, please use the form on the Contact Page.
In order to understand more about plant telomere biology, we set out to identify genetic factors associated with telomere length regulation in maize (Zea mayz L.), a model genetic system. Using terminal restriction fragment (TRF) analysis, we measured telomere lengths from diverse maize inbred lines as well as the recombinant inbred lines (RILs) from the intermated B73 x Mo17 (IBM) mapping population. Maize telomere length was found to be diverse, ranging from 2 kb to more than 20 kb. The telomere lengths of the RILs were subjected to quantitative trait locus (QTL) analysis in order to identify markers co-segregating with telomere length. Significant associations were detected on nine of the ten chromosomes, with two loci showing evidence of an epistatic relationship. QTL intervals were examined for candidate genes involved in telomere metabolism. As a first step in validation of candidate genes, a subset of candidates was assayed for gene expression levels correlating with telomere length. The results of this analysis not only supported predicted functions of some candidate genes, but also indicated that a RAD51-like protein likely interacts with another telomeric protein in some aspect of telomere length regulation in maize. These data open a new avenue for exploring fundamental aspects of telomere biology in plants using the diverse model genetic system, maize.
A Dissertation submitted to the Department of Biological Science in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
Includes bibliographical references.
Hank Bass, Professor Directing Dissertation; Cathy Levenson, University Representative; Lloyd Epstein, Committee Member; Laura Keller, Committee Member; Hong-Guo Yu, Committee Member.
Florida State University
Use and Reproduction
This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). The copyright in theses and dissertations completed at Florida State University is held by the students who author them.