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- Title
- Legality, Ethics and Memory: The Role of American Physicians in Post-WWII Germany.
- Creator
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Abel, Liesa A.
- Abstract/Description
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This thesis addresses the responses and subsequent roles of American military physicians in Post- WWII Germany, from March 1945 to January 1950. Physician roles include medical practice both within and outside of their specialties, hospital administration, and work in Prisoner of War (POW) and Displaced Persons (DP) camps. Physicians treated trauma in their patients but also experienced trauma themselves; they also wrestled with conflicting feelings toward German civilians and former Nazis....
Show moreThis thesis addresses the responses and subsequent roles of American military physicians in Post- WWII Germany, from March 1945 to January 1950. Physician roles include medical practice both within and outside of their specialties, hospital administration, and work in Prisoner of War (POW) and Displaced Persons (DP) camps. Physicians treated trauma in their patients but also experienced trauma themselves; they also wrestled with conflicting feelings toward German civilians and former Nazis. Explored here are altruistic motives of physicians who treated POWs and DPs, as well as a contemporary assessment of the significance of their work.
Show less - Date Issued
- 2017-04-28
- Identifier
- FSU_libsubv1_scholarship_submission_1493385335
- Format
- Thesis
- Title
- A Colorful Investigation: Deoxo Rhodamine Spirolactam Ring Opening Rates and The Development of New Rhodamine Dyes based on ESIPT.
- Creator
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Accardo, Joseph, Zhu, Lei
- Abstract/Description
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Deoxo rhodamine spirolactams (DRS) undergo significant fluorescence enhancement when going from their neutral (closed from) to dicationic state (open form). New and existing DRS were synthesized through the reduction of rhodamine spirolactams (RS). The pKa values for the two protonation processes were determined for a set of three DRS, and the overall rates were measured by the stopped-flow method. These compounds exhibited high ring opening rates, up to 104 times as fast as reported RS...
Show moreDeoxo rhodamine spirolactams (DRS) undergo significant fluorescence enhancement when going from their neutral (closed from) to dicationic state (open form). New and existing DRS were synthesized through the reduction of rhodamine spirolactams (RS). The pKa values for the two protonation processes were determined for a set of three DRS, and the overall rates were measured by the stopped-flow method. These compounds exhibited high ring opening rates, up to 104 times as fast as reported RS probes for the same reaction. In addition to the investigation, a fluorescent study of 1,2,3-triazoles is also described, which has fueled the future directions of this study.
Show less - Date Issued
- 2016-04-22
- Identifier
- FSU_libsubv1_scholarship_submission_1461352325
- Format
- Thesis
- Title
- A meal pattern and time-course analysis of estrogen receptor agonists.
- Creator
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Adams, Kia, Butler, Michael, Eckel, Lisa
- Abstract/Description
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Estradiol (E2) is an ovarian hormone that has a well-characterized anorexigenic effect in female animals that was originally believed to be mediated through the activation of nuclear estrogen receptors (ERs). However, recent studies from our lab and others have shown rapid anorexigenic effects after activation of membrane ERs (mERs), such as mER and G-protein coupled estrogen receptor (GPER). The comparative action of the non-selective ER agonist, estradiol benzoate (EB), and the selective ER...
Show moreEstradiol (E2) is an ovarian hormone that has a well-characterized anorexigenic effect in female animals that was originally believed to be mediated through the activation of nuclear estrogen receptors (ERs). However, recent studies from our lab and others have shown rapid anorexigenic effects after activation of membrane ERs (mERs), such as mER and G-protein coupled estrogen receptor (GPER). The comparative action of the non-selective ER agonist, estradiol benzoate (EB), and the selective ER and GPER agonists PPT and G-1, respectively, is poorly understood. In the current study, we analyzed meal patterns after acute administration of each of these agonists in OVX female Long-Evans rats. Both PPT and G-1 produced rapid decreases in food intake within 2 and 1 h, respectively, with associated decreases in the size of the first meal following drug treatment. It was also determined that EB produces a prolonged anorexigenic effect, suppressing food intake for three days beginning 12 h after drug treatment. Overall, these findings provide additional evidence that activation of mERs alone is sufficient to decrease food intake and that mER agonists produce more rapid but more transient effects than the non-selective ER agonist EB.
Show less - Date Issued
- 2017-04-28
- Identifier
- FSU_libsubv1_scholarship_submission_1493409498
- Format
- Thesis
- Title
- The Effect of the Center of Academic Retention and Enhancement on the College Enrollment of Black Undergraduate Students at the Florida State University.
- Creator
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Brown, Keturah, African-American Studies
- Abstract/Description
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College enrollment rates into institutions of higher education have increased over the last thirty years for Black high school graduates (Insitute of Education Science, 2011). However, there are many college enrollment factors that influence a student's decision to attend a college or university. This study sought to determine the most influential college enrollment factors that affect the decision of Black undergraduates to enroll at FSU. Specifically, the study analyzed the role that the...
Show moreCollege enrollment rates into institutions of higher education have increased over the last thirty years for Black high school graduates (Insitute of Education Science, 2011). However, there are many college enrollment factors that influence a student's decision to attend a college or university. This study sought to determine the most influential college enrollment factors that affect the decision of Black undergraduates to enroll at FSU. Specifically, the study analyzed the role that the Center of Academic Retention and Enhancement, a summer bridge program at the Florida State University, plays in the enrollment of Black undergraduate students. A survey questionnaire was developed and distributed to Black undergraduate students at Florida State University that asked questions concerning the importance of various college enrollment factors. The study found that "Financial Aid: Scholarships and Grants" was the most important college enrollment factor. The C.A.R.E Program was also influential in the decision of the majority of C.A.R.E students to attend FSU. Based off of the findings from the survey, it is evident that the C.A.R.E Program is a vital instrument in the recruitment of Black undergraduate students to FSU and to the academic success of those students throughout their college career.
Show less - Date Issued
- 2012
- Identifier
- FSU_migr_uhm-0099
- Format
- Thesis
- Title
- Investigating circadian modulation of consummatory learning using a classical paradigm in Aplysia.
- Creator
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Allem, Alyssa D.
- Abstract/Description
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The circadian system is an endogenous system of oscillators that rhythmically regulate many biological processes. Memory formation is a prominent process influenced by the circadian clock. Learning and memory are vital processes for any organism to make predictions about certain aspects of their environment. Aplysia have been a significant model organism for studying memory and circadian rhythms, as they demonstrate certain behaviors with fluctuation based on the time of day. Aplysia...
Show moreThe circadian system is an endogenous system of oscillators that rhythmically regulate many biological processes. Memory formation is a prominent process influenced by the circadian clock. Learning and memory are vital processes for any organism to make predictions about certain aspects of their environment. Aplysia have been a significant model organism for studying memory and circadian rhythms, as they demonstrate certain behaviors with fluctuation based on the time of day. Aplysia californica is a diurnal species that only sleeps at night, similar to humans, meaning that they would presumably feed during the day. In previous studies, feeding behavior of Aplysia has provided a great model system for investigating consummatory learning, as new learning paradigms that focus on biting activity have emerged to study classical conditioning in Aplysia. However, there has been a lack in research investigating the circadian aspects of consummatory feeding behaviors in Aplysia and whether or not consummatory learning is shaped by circadian oscillators. This study focused on characterizing consummatory feeding behaviors in Aplysia using a behavioral biting assay time course and it was concluded that biting response is regulated by the circadian clock. However, associative paradigms that measure biting response may not be suitable to investigate circadian modulation of learning and memory. Determining how the circadian clock regulates biting activity is valuable to A. californica’s use as a model organism for studying the circadian modulation of learning.
Show less - Date Issued
- 2016-12-06
- Identifier
- FSU_libsubv1_scholarship_submission_1481005091
- Format
- Thesis
- Title
- Characterization of small molecule inhibitors of Zika Virus infection.
- Creator
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Allen, Chase Terrance
- Abstract/Description
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Zika virus (ZIKV) is a mosquito-transmitted positive RNA sense virus that has spread rapidly in the past year, prompting the World Health Organization (WHO), on 1 February 2016, to announce that the association of Zika infection with clusters of microcephaly and other neurological disorders constituted a Public Health Emergency of International Concern1. The state of emergency eventually ended in November of 2016; however, given the global health concern posed by this virus, a large-scale...
Show moreZika virus (ZIKV) is a mosquito-transmitted positive RNA sense virus that has spread rapidly in the past year, prompting the World Health Organization (WHO), on 1 February 2016, to announce that the association of Zika infection with clusters of microcephaly and other neurological disorders constituted a Public Health Emergency of International Concern1. The state of emergency eventually ended in November of 2016; however, given the global health concern posed by this virus, a large-scale effort to identify drugs for treating ZIKV and combatting its continued spread is of utmost importance. There are currently no drugs or vaccines for the treatment or prevention of ZIKV infection, although at least one vaccine study is scheduled for stage II/IIb clinical trials. This vaccine is currently being developed by the National Institute of Allergy and Infectious Diseases (NIAID), a branch of the National Institutes of Health (NIH). Although this vaccine study is underway, it is still highly advantageous to have small molecule inhibitors because whether or not this vaccination will produce susceptibilities to secondary flavivirus infections is still unknown42. The urgency that this situation provides led us and others to look to test preexisting drugs for efficacy against ZIKV infection. In attempt to close this gap in treatment and prevention we, in collaboration with Dr. Wei Zheng and others at the National Center for Advanced Translational Sciences (NCATS), previously reported a screen of 6,000 compounds for anti-ZIKV activity. In this study, we identified several compounds with sub-micromolar anti-ZIKV activity in a cell-culture based model, including the FDA approved anthelmintic drug Niclosamide. Niclosamide proved to be capable of inhibiting ZIKV replication at ~0.2μM concentrations, in vitro, when added either before or after ZIKV exposure2. In order to pursue additional potential drugs, our collaborative efforts have, in total, screened over 80,000 compounds for anti-ZIKV activity. Of these, we have selected several compounds for characterization, based on multiple factors: initial antiviral activity in a ZIKV viral protein (non-structural protein 1) detection assay, cytotoxicity profiles, and US Food and Drug Administration (FDA) approval status or previous in vivo safety profiles via human clinical trials. Attempts to further characterize the anthelmintic drug Niclosamide as well as efforts to identify several more compounds for antiviral activity were explored in a cell culture based ZIKV model for efficacy against ZIKV infection and drug anti-ZIKV mechanism of action. My results demonstrate the efficacy of these screening techniques, identify potential compounds for anti-ZIKV drug development, and provide preliminary insights on the selected drug mechanisms of action.
Show less - Date Issued
- 2017-04-27
- Identifier
- FSU_libsubv1_scholarship_submission_1493319376
- Format
- Thesis
- Title
- Syntaxin3 is Essential for Photoreceptor Survival in Zebrafish.
- Creator
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Allen, Dylan Michael
- Abstract/Description
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SNARE proteins are essential for membrane fusion in eukaryotes. Syntaxin binding proteins (STXBP) mediate SNARE-dependent vesicular binding to the plasma membrane. A mutation was previously isolated in stxbp1b resulting in rapid degeneration of retinal photoreceptors. I hypothesize that Stx3, a member of the SNARE complex, is also essential for photoreceptor survival. Mutations were induced in stx3 by injecting gRNA targeting exon 2 and mRNA encoding Cas9 into 1-cell stage zebrafish embryos....
Show moreSNARE proteins are essential for membrane fusion in eukaryotes. Syntaxin binding proteins (STXBP) mediate SNARE-dependent vesicular binding to the plasma membrane. A mutation was previously isolated in stxbp1b resulting in rapid degeneration of retinal photoreceptors. I hypothesize that Stx3, a member of the SNARE complex, is also essential for photoreceptor survival. Mutations were induced in stx3 by injecting gRNA targeting exon 2 and mRNA encoding Cas9 into 1-cell stage zebrafish embryos. Embryos and adults were genotyped using PCR and DNA sequencing. Retinal phenotypes were characterized for visual deficits using the optokinetic reflex (OKR). Histological sections were immunolabeled for photoreceptor-specific antigens. Using this approach, I isolated novel alleles of stx3. One allele is predicted to result in a frame shift mutation and premature stop codon. 25% of the offspring from matings between 2 heterozygous carriers of the novel stx3 allele failed to display an OKR. Larvae lacking an OKR were homozygous for the mutation, and immunolabeling revealed photoreceptor degeneration. These data show that the SNARE complex is essential for photoreceptor survival in the developing zebrafish retina. Supported by the NIH and FSU.
Show less - Date Issued
- 2018-10-2018
- Identifier
- FSU_libsubv1_scholarship_submission_1533830132_1751809e
- Format
- Thesis
- Title
- Perceptions of Racism Among College Students: Race, Gender, and the Influence of Social Media.
- Creator
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Amaya, Kristen
- Abstract/Description
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This thesis analyzes internal perceptions of institutional racism among undergraduate college students. The research examines the degree to which students perceive the issue of institutional racism to be an existing and pressing problem in our society today. The paper explores how students' perceptions of racism may be influenced by their race, gender, and social attitudes. The thesis also analyzes how students' perceptions of racism are mediated by access and exposure to the vast amounts of...
Show moreThis thesis analyzes internal perceptions of institutional racism among undergraduate college students. The research examines the degree to which students perceive the issue of institutional racism to be an existing and pressing problem in our society today. The paper explores how students' perceptions of racism may be influenced by their race, gender, and social attitudes. The thesis also analyzes how students' perceptions of racism are mediated by access and exposure to the vast amounts of political information available on social media sites such as Twitter and Facebook. A survey of 110 college students was conducted and both quantitative and qualitative data were collected. Quantitative results suggest that students' awareness of racial inequality and racial discrimination were not influenced by race or gender identity but were influenced by their social attitudes and the nature of their social media use. Qualitative results provide an in-depth analysis of students' personal opinions about racism and suggest an overall awareness of the issue, but nonetheless polarized views.
Show less - Date Issued
- 2017-12-11
- Identifier
- FSU_libsubv1_scholarship_submission_1513037496_a2cdd06a
- Format
- Thesis
- Title
- Interface Binding of the Escherichia coli Sulfite Reductase Flavoprotein Subunit.
- Creator
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Andrews, Rachel
- Abstract/Description
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Sulfite Reductase is an enzyme that catalyzes the six-electron reduction of sulfite to sulfide for assimilation into biological sulfur-containing compounds, like L-cysteine for protein formation. The Sulfite Reductase holoenzyme is composed of eight copies of the flavoprotein (SiRFP, α subunit) and four copies of the hemoprotein (SiRHP, β subunit) to make a 780 kDa metalloenzyme. SiRFP contains two flavin cofactors, FAD and FMN, which shuttle electrons from NADPH onto the metal core of SiRHP....
Show moreSulfite Reductase is an enzyme that catalyzes the six-electron reduction of sulfite to sulfide for assimilation into biological sulfur-containing compounds, like L-cysteine for protein formation. The Sulfite Reductase holoenzyme is composed of eight copies of the flavoprotein (SiRFP, α subunit) and four copies of the hemoprotein (SiRHP, β subunit) to make a 780 kDa metalloenzyme. SiRFP contains two flavin cofactors, FAD and FMN, which shuttle electrons from NADPH onto the metal core of SiRHP. SiRHP uses the electrons from SiRFP in coordination with its two cofactors, siroheme and an Fe4S4 cluster, to reduce sulfite. How these two subunits assemble has not been fully characterized. Recent data shows the N-terminal 80 amino acids of SiRHP are necessary for complex formation, and one region of SiRFP is suspected of being important for complex formation. Site-directed mutagenesis was used to make SiRFP variants in that region to identify which amino acid residues are responsible for facilitating the formation of the holoenzyme. Point mutants were also made in the N-terminal FMN domain to test which amino acids are necessary for SiRFP function. In vivo analysis through complementation assays showed the variants retained the ability to reduce sulfite. Purification was completed using a nickel affinity column and size exclusion to perform analytical size exclusion.
Show less - Date Issued
- 2016-04-21
- Identifier
- FSU_libsubv1_scholarship_submission_1461260110
- Format
- Thesis
- Title
- Implementing Bootstrap Resampling for Sequential Process Monitoring of Functional Data.
- Creator
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Archer, Andrew J.
- Abstract/Description
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In many fields of statistical research, statisticians are challenged in producing risk limited, bias controlled data near instantaneously. For instance, in the area of signal detection, there exists a need to process mass quantities of data in a short time frame while simultaneously detecting the time at which a change in the process occurs. This has been a daunting task for many analytics professionals. Hawkins et al. (2003) outlined the traditional implementation of control charts for...
Show moreIn many fields of statistical research, statisticians are challenged in producing risk limited, bias controlled data near instantaneously. For instance, in the area of signal detection, there exists a need to process mass quantities of data in a short time frame while simultaneously detecting the time at which a change in the process occurs. This has been a daunting task for many analytics professionals. Hawkins et al. (2003) outlined the traditional implementation of control charts for process monitoring. Charts including the cumulative sum and exponentially weighted moving average control chart were introduced as standard methods for identifying and monitoring process shifts. Unfortunately, these methods are limited by the need to estimate parameters, or are limited by simplifying parameters and known error distributions. To confront this challenge, we propose the use of a nonparametric method for process modeling of functional data that incorporates a variety of resampling methods.
Show less - Date Issued
- 2017-04-27
- Identifier
- FSU_libsubv1_scholarship_submission_1493346509
- Format
- Thesis
- Title
- An Empirical Study On The Social Protection Program "Red De Oportunidades" In Panama.
- Creator
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Archila, Juan Andres
- Abstract/Description
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In this thesis, I evaluate the impact that social protection program "Red De Oportunidades" (RDO) had on a variety of socio-economic outcomes in Panama. These include, but are not limited to the poverty rate, income, education, and health. Results describe a relatively small negative effect on the outcomes of interest. However, the measurements do not immediately translate to a definitive statement about the effects of RDO. Without controlling for fixed effects, the coefficient for all...
Show moreIn this thesis, I evaluate the impact that social protection program "Red De Oportunidades" (RDO) had on a variety of socio-economic outcomes in Panama. These include, but are not limited to the poverty rate, income, education, and health. Results describe a relatively small negative effect on the outcomes of interest. However, the measurements do not immediately translate to a definitive statement about the effects of RDO. Without controlling for fixed effects, the coefficient for all dependent variables is overstated; highlighting the importance of controlling for unobservable variables. Due to data problems and time constraints, the results do not provide a conclusive statement about the effects of RDO, but they provide insight into the challenges of studying the broad subject of poverty and the complex effects of conditional cash transfer programs like RDO in different contexts.
Show less - Date Issued
- 2018-04-23
- Identifier
- FSU_libsubv1_scholarship_submission_1524502979_a0e33c0f
- Format
- Thesis
- Title
- Studies of Mechanisms Underlying Cell Competition Induced by Imbalance of Energy Metabolism.
- Creator
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Arrington, Brooke Aileen
- Abstract/Description
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Abstract Adenosine triphosphate (ATP) is the molecular currency for cells of all living organisms. When its synthesis, through ATP Synthase, is inhibited or “knocked down” cellular metabolic pathways will be compromised and, I hypothesize that, cell competition will result. Cell competition is a method by which tissue integrity and homeostasis is maintained. When neighboring cells differ in cell fitness, the fit cells will outcompete the weakened cells, leading to cell competition, and...
Show moreAbstract Adenosine triphosphate (ATP) is the molecular currency for cells of all living organisms. When its synthesis, through ATP Synthase, is inhibited or “knocked down” cellular metabolic pathways will be compromised and, I hypothesize that, cell competition will result. Cell competition is a method by which tissue integrity and homeostasis is maintained. When neighboring cells differ in cell fitness, the fit cells will outcompete the weakened cells, leading to cell competition, and therefore, tissue homeostasis. In this experiment, I explored the consequences of depriving cells of ATP Synthase. In order to see cell competition, a mosaic condition using the GeneSwitch Gal4 system was created, consisting of wild type and ATP Synthase knockdown cells. Using the Gal80TS System, a control was also set up creating a homogenous condition of only ATP Synthase knock down cells. Cell competition was visualized using DCP-1 (death caspase-1) staining which targets apoptotic cells. Apoptosis was seen in the mosaic condition, where cells of different fitness neighbored one another. No cell competition was seen in the homogenous condition. The intracellular signaling pathways followed during cell competition were then explored, comparing the deprivation of ATP to the deprivation of insulin. tGPH, which fluoresces green under a confocal microscope, was used in order to trace levels of PI3K following ATP Synthase knock down. When cells are deprived of insulin, tGPH localization to the cell membrane decreases substantially. Similar results were seen in this experiment when cells are deprived of ATP. This result leads to the hypothesis that the insulin and ATP intracellular signaling pathways may be similar or connected. These results allow for further research in this field of interest.
Show less - Date Issued
- 2016-04-21
- Identifier
- FSU_libsubv1_scholarship_submission_1461261313
- Format
- Thesis
- Title
- Seasonal changes in male size and alternative mating tactics in sailfin mollies (Poecilia latipinna).
- Creator
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Arriola Mendieta, Ivonne, Lange, Elizabeth C., Hughes, Kimberly A.
- Abstract/Description
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In many taxa, individuals display discrete variation in both morphology and behavior. Although these “alternative tactics” are relatively common, their maintenance within populations has yet to be adequately explained. A first step in addressing this question is to describe how behavior changes within a population over time. Sailfin mollies (Poecilia latipinna) are a livebearing fish that show extreme variation in mature male size and reproductive behavior. Adult male size is genetically...
Show moreIn many taxa, individuals display discrete variation in both morphology and behavior. Although these “alternative tactics” are relatively common, their maintenance within populations has yet to be adequately explained. A first step in addressing this question is to describe how behavior changes within a population over time. Sailfin mollies (Poecilia latipinna) are a livebearing fish that show extreme variation in mature male size and reproductive behavior. Adult male size is genetically determined but highly variable within populations, and reproductive behavior covaries with genetically-determined size. Large males display courtship behavior, small males use sneaking behavior, and males in the middle of the size distribution exhibit behavioral plasticity that depends on the social context. Large males also mature more slowly than small males, so the size distribution of adult males within a population can vary dramatically over time. To determine if temporal variation and behavioral plasticity could play a role in maintaining these polymorphisms, we asked if individual male behavior covaries with the male body size distribution across seasons within a population. We censused the population and recorded the size of males, females and juveniles monthly from February 2015 to January 2017. A subset of males, from fish censused in June and October 2015-2016, was brought to the lab to assess reproductive behavior towards a receptive female. We found that the male size distribution varied seasonally; males were largest in the fall and winter, and smallest in late spring and summer. Standard length and its interaction with season significantly affected the male rate of courtship displays, but not sneaking behavior or gonoporal nibbles. This result suggests that changes in individual male behavior track the male size distribution within populations, recapitulating a pattern that has been shown across populations.
Show less - Date Issued
- 2017-04-28
- Identifier
- FSU_libsubv1_scholarship_submission_1493399548
- Format
- Thesis
- Title
- Charles IV: Religious Propaganda and Imperial Expansion.
- Creator
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Crowley, Stephanie, Art History
- Abstract/Description
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The Bohemian Charles IV (1316 – 1378) was crowned King of Bohemia in 1347, King of the Romans in 1349, and Holy Roman Emperor in 1355. At the time of his death, he had successfully expanded the borders of the Holy Roman Empire to include the Kingdom of Bohemia, the Kingdom of Burgundy, the Duchy of Pomerania, and he had himself crowned King of Lombardy. The artwork Charles IV commissioned played a major legitimizing role in this imperial expansion. My study investigates the artistic program...
Show moreThe Bohemian Charles IV (1316 – 1378) was crowned King of Bohemia in 1347, King of the Romans in 1349, and Holy Roman Emperor in 1355. At the time of his death, he had successfully expanded the borders of the Holy Roman Empire to include the Kingdom of Bohemia, the Kingdom of Burgundy, the Duchy of Pomerania, and he had himself crowned King of Lombardy. The artwork Charles IV commissioned played a major legitimizing role in this imperial expansion. My study investigates the artistic program of Charles IV in relation to his active promotion of religious cults devoted to three carefully selected saints; St. Wenceslas, St. Charlemagne, and St. Sigismund. I argue that the emperor employed a widespread and calculated artistic program to lay the foundations for his dynasty by creating strong visual ties between himself, his heirs, and the aforementioned royal saints while simultaneously promoting local devotion to those saints. In a detailed examination of the Crowned Reliquaries of Charles IV, the Holy Cross Chapel, and the Madonna of John Očko of Vlašim, I will prove the effectiveness of the emperor's expansive artistic campaign in shaping the way he was perceived in contemporary society, despite his contested ascent to the Bohemian and imperial thrones. I argue that the widespread artistic program of Charles IV was ultimately successful because, by the end of his rule, propagandistic themes common to artwork commissioned by the emperor were present in privately commissioned artwork as well.
Show less - Date Issued
- 2011
- Identifier
- FSU_migr_uhm-0008
- Format
- Thesis
- Title
- E42: Architecture and the Visual Culture of Fascist Italy.
- Creator
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Ciampittiello, Masha, Art History
- Abstract/Description
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During the reign of Benito Mussolini (1925–1943), Italian architects reevaluated the purpose and direction that modernism had taken in Italy, reorienting its previous focus on progressive, functional architecture for the masses to include political propaganda for the state. From the Novecento, Rationalist, and Futurist movements, the Regime chose designs that referred back to the stark monumentality of Imperial Rome. This thesis argues that despite such constraints, Italian architects managed...
Show moreDuring the reign of Benito Mussolini (1925–1943), Italian architects reevaluated the purpose and direction that modernism had taken in Italy, reorienting its previous focus on progressive, functional architecture for the masses to include political propaganda for the state. From the Novecento, Rationalist, and Futurist movements, the Regime chose designs that referred back to the stark monumentality of Imperial Rome. This thesis argues that despite such constraints, Italian architects managed to find innovative solutions and novel forms for representing Italian fascist rhetoric, producing a great deal of individual variation within the architectural schools with which they were associated. Support for Mussolini's empire rested on popular mythologizing about the former Roman Empire and the belief among Italians that the nation and its people were destined to revisit its glory. The propagandistic forms designed by Italian architects employed by the state, as I argue, reflect this understanding as to the role of classical heritage in the present and the place of individual innovation. The principal evidence for my claim is the architecture and urban planning associated with the aborted Esposizione universale of 1942 (colloquially known as E42). The state used this suburban network of exhibition halls to display the supremacy of contemporary Italian culture by making reference to classical Roman antiquity and to a lesser extent the monumental forms of the Renaissance. My study investigates the ways in which the architects of E42, in seeking to integrate the masses on a personal level with the political ideology of the state, referenced Roman antiquity and the Renaissance, thereby visualizing connections between Mussolini's empire and the successful authoritative governments of Italy's past. I provide a detailed assessment of E42 and the various architectural schools competing for state sponsorship in order to demonstrate that the production of Italian fascist visual culture was contingent to a greater degree than has previously been acknowledged upon the notion that the state fostered some measure of individualism in artistic design.
Show less - Date Issued
- 2012
- Identifier
- FSU_migr_uhm-0095
- Format
- Thesis
- Title
- Music Education in the Margins: A Case Study of the Guri Santa Marcelina.
- Creator
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Aycock, Sylvia Rose
- Abstract/Description
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Beyond teaching students to sing and play instruments in an ensemble, many community music organizations aim to teach discipline and mold character. One such organization, the Guri Santa Marcelina located in São Paulo, Brazil, provides a social justice-driven music education in which students of all backgrounds and circumstances are afforded the opportunity to study music, something the organization sees as a fundamental right. Employing both music educators and social workers, the Guri Santa...
Show moreBeyond teaching students to sing and play instruments in an ensemble, many community music organizations aim to teach discipline and mold character. One such organization, the Guri Santa Marcelina located in São Paulo, Brazil, provides a social justice-driven music education in which students of all backgrounds and circumstances are afforded the opportunity to study music, something the organization sees as a fundamental right. Employing both music educators and social workers, the Guri Santa Marcelina attends to the needs of the students holistically in and outside of the classroom working with families and the community as a whole. The organization currently has forty-six satellite locations throughout the city of twenty million. In participating in music classes and activities, and interviewing teachers and social workers at the Guri Santa Marcelina, I had the opportunity to learn how their mission plays out in reality. How do the social workers meet the students’ and families’ needs? How are the music teachers educating their students? How do the social workers and music teachers interact and influence one another? Through ethnographic research, I examine the relationship between music education and social justice in the example of the Guri Santa Marcelina, a music education institution, and social project. Contextualizing my interactions and reflections with a review of the literature, I explore the possibility of creating social change through music education.
Show less - Date Issued
- 2016-04-28
- Identifier
- FSU_libsubv1_scholarship_submission_1461847664
- Format
- Thesis
- Title
- Relationship between Vocabulary Knowledge and Morphological Awareness in English Learners.
- Creator
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Baldauf, Nicole Sue
- Abstract/Description
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This study examined the relationship between vocabulary knowledge and morphological awareness. It was thought that these two measures would be positively correlated. There were 35 participants from a summer migrant education program ranging in age from fourth to seventh grade. There were three measures of morphological awareness and two measures of vocabulary knowledge that were examined for correlation. The results showed that there was in fact a positive correlation between each of the...
Show moreThis study examined the relationship between vocabulary knowledge and morphological awareness. It was thought that these two measures would be positively correlated. There were 35 participants from a summer migrant education program ranging in age from fourth to seventh grade. There were three measures of morphological awareness and two measures of vocabulary knowledge that were examined for correlation. The results showed that there was in fact a positive correlation between each of the compared morphological awareness and vocabulary knowledge tasks. These correlations ranged in strength from moderately to strongly correlated. Future research should also focus on the population of English Learners as it is underrepresented however, it should be causal in order to inform clinicians and teachers on the best method in which to aid English Learners.
Show less - Date Issued
- 4/27/2018
- Identifier
- FSU_libsubv1_scholarship_submission_1524843199_54d787c8
- Format
- Thesis
- Title
- Serving Up a Social Movement: The Real Food Challenge at Florida State University.
- Creator
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Baldwin, Serena Justine
- Abstract/Description
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The modern food system, rooted in industrialized agricultural practices and corporate consolidation of the agribusiness sector, has contributed to numerous adverse public health, environmental, and social consequences. With higher education foodservice spending amounting to over $5 billion and leading foodservice industry trends, colleges and universities are uniquely positioned to challenge the power structure of the food system. The Real Food Challenge (RFC) is a national student movement...
Show moreThe modern food system, rooted in industrialized agricultural practices and corporate consolidation of the agribusiness sector, has contributed to numerous adverse public health, environmental, and social consequences. With higher education foodservice spending amounting to over $5 billion and leading foodservice industry trends, colleges and universities are uniquely positioned to challenge the power structure of the food system. The Real Food Challenge (RFC) is a national student movement that aims to shift 20% of existing higher education food budgets away from and industrial farms and highly processed food and toward the procurement of local and community based, ecologically sound, fair, and humane food sources, collectively referred to as “real food,” by 2020. This analysis employs a social movements theoretical framework to outline the best practices for igniting and sustaining a student-driven Real Food Movement at Florida State University (FSU), assesses support for the RFC among key stakeholders, and draws on the limitations of the RFC by identifying alternative sustainable food initiatives being implemented at FSU’s peer institutions.
Show less - Date Issued
- 12/08/2017
- Identifier
- FSU_libsubv1_scholarship_submission_1512766883_f9551b5a
- Format
- Thesis
- Title
- Meiofauna and Sediment: How Abundances Differ Based on Grain Size.
- Creator
-
Ballentine, William Michael
- Abstract/Description
-
Meiofauna are the nearly microscopic organisms that live in the interstitial spaces between individual granules of sediment. They are found all over the world in almost every place where water meets earth. Because their environment is made up of the surrounding sediment, it is understandable that the grain size of that sediment is a very important factor in the lives of these tiny organisms. I have counted and categorized the communities of nematodes and harpacticoid copepods in two...
Show moreMeiofauna are the nearly microscopic organisms that live in the interstitial spaces between individual granules of sediment. They are found all over the world in almost every place where water meets earth. Because their environment is made up of the surrounding sediment, it is understandable that the grain size of that sediment is a very important factor in the lives of these tiny organisms. I have counted and categorized the communities of nematodes and harpacticoid copepods in two ecologically different sites that are ~256m apart. I have quantified the average grain size for both sites, a saltmarsh (182.69 µm) and a seagrass bed (286.66µm), and have attempted to fit it into an ecological framework for the nematode/copepod communities that live there. I found that between these two habitats, both nematode and copepod communities increased with grain size and that despite ecological differences, the ratio of copepods to nematode in the two sites was not significantly different.
Show less - Date Issued
- 2016-04-22
- Identifier
- FSU_libsubv1_scholarship_submission_1461350426
- Format
- Thesis
- Title
- Characterization of Photo-Oxidized Wine by FT-ICR MS.
- Creator
-
Bartges, Tessa Eve
- Abstract/Description
-
This thesis presents the first investigation of compositional changes in wine caused by photo-degradation characterized by high-resolution mass spectrometry. Native, dark, and irradiated red and white wine samples were analyzed by negative electrospray Fourier transform ion cyclotron resonance mass spectrometry and excitation-emission matrix spectroscopy. Significant changes in nitrogen-containing heteroatom classes where observed in both wines after irradiation. The irradiated white wine was...
Show moreThis thesis presents the first investigation of compositional changes in wine caused by photo-degradation characterized by high-resolution mass spectrometry. Native, dark, and irradiated red and white wine samples were analyzed by negative electrospray Fourier transform ion cyclotron resonance mass spectrometry and excitation-emission matrix spectroscopy. Significant changes in nitrogen-containing heteroatom classes where observed in both wines after irradiation. The irradiated white wine was found to contain less high oxygen number classes than the native or dark white wines. The irradiated red wine was found to contain higher oxygen number classes than the native or dark red wines.
Show less - Date Issued
- 2016-04-21
- Identifier
- FSU_libsubv1_scholarship_submission_1461259640
- Format
- Thesis
- Title
- A Day in the Life of Picoplankton in Dickerson Bay, FL.
- Creator
-
Basden, Isabelle G
- Abstract/Description
-
Pelagic ecosystems play an important role in regulating the Earth’s biogeochemical processes. Picoplankton, cells > 2µm, are some of the most abundant plankton in the pelagic community and responsible for 44-90% of primary production in tropical oceans. This study attempts to understand the daily fluctuations of picoplanktonic organisms in relation to the abiotic and biotic factors that may influence their dynamics by 1) analyzing the fluctuations in picoplankton community structure and...
Show morePelagic ecosystems play an important role in regulating the Earth’s biogeochemical processes. Picoplankton, cells > 2µm, are some of the most abundant plankton in the pelagic community and responsible for 44-90% of primary production in tropical oceans. This study attempts to understand the daily fluctuations of picoplanktonic organisms in relation to the abiotic and biotic factors that may influence their dynamics by 1) analyzing the fluctuations in picoplankton community structure and density from 8am to 8pm 2) analyzing the relationship between picoplankton fluctuations and abiotic factors including light, temperature, and the tidal cycle and 3) analyzing the impact of a sponge filter feeder on picoplankton abundance. Picoplankton densities fluctuated significantly through out the day and tidal cycles and vertical mixing could play a large role in these daily dynamics. Sponge feeding can significantly decrease the density of autotrophic picoplankton and could be a form of control for picoplankton communities.
Show less - Date Issued
- 2018-12-06
- Identifier
- FSU_libsubv1_scholarship_submission_1544132166_3fcd2e46
- Format
- Thesis
- Title
- Reading Ability and Substance Use in Adolescents: A Twin Study.
- Creator
-
Bater, Devan
- Abstract/Description
-
Through the study of vulnerability and early risk factors, the increased chance of those with reading disabilities suffering from substance abuse can be possibly prevented and better ways to cope can be discovered. Previous studies have shown that low reading scores and abilities in adolescence can be an early sign of possible reading disorders and substance use in adolescence can be a risk factor for substance abuse later in life. The focus of this study was to explore the correlation...
Show moreThrough the study of vulnerability and early risk factors, the increased chance of those with reading disabilities suffering from substance abuse can be possibly prevented and better ways to cope can be discovered. Previous studies have shown that low reading scores and abilities in adolescence can be an early sign of possible reading disorders and substance use in adolescence can be a risk factor for substance abuse later in life. The focus of this study was to explore the correlation between different reading ability measures and a substance use measure to determine if there is a correlation between the two risk factors. A twin study was also used to determine if any possible correlations were due to genetic factors, environmental factors, or both. This study was conducted using adolescent twin data collected from the Florida Twin Project on Reading, Behavior, and Environment. The substance use measure in this study focused primarily on alcohol use. It was hypothesized that lower reading abilities would mean a higher probability of substance use, and that this correlation would be caused by genetic factors. The results showed very few phenotypic correlations with statistical significance between the reading ability measures and the substance use measure. The twin correlations for the substance use measure proved to show the correlations with the most statistical significance. Only one cross-twin cross-trait correlation was found to be statistically significant between the reading ability measures and the substance use measure. The findings in this study do not provide evidence to support nor dispute the hypothesis since there did not appear to be a clear positive nor negative relationship between low reading ability and substance use in adolescents. The results did suggest non-additive genetics could be a causal effect on the relationship of substance use between twin pairs.
Show less - Date Issued
- 2018-04-04
- Identifier
- FSU_libsubv1_scholarship_submission_1524848029_b28c57d3
- Format
- Thesis
- Title
- Malleability of Measures of Cognitive Ability.
- Creator
-
Bayles, Megan Ashley
- Abstract/Description
-
“Brain training” claims have been the subject of many recent investigations. Proponents of brain training believe that practicing simple cognitive exercises or gamified versions of these exercises can result in improvements not just to the practiced task, but to other tasks and abilities as well. This has been termed “transfer of training.” For example, Jaeggi, Buschkuehl, Jonides, and Perrig (2008) have claimed that since working memory is vital to the performance of many different tasks,...
Show more“Brain training” claims have been the subject of many recent investigations. Proponents of brain training believe that practicing simple cognitive exercises or gamified versions of these exercises can result in improvements not just to the practiced task, but to other tasks and abilities as well. This has been termed “transfer of training.” For example, Jaeggi, Buschkuehl, Jonides, and Perrig (2008) have claimed that since working memory is vital to the performance of many different tasks, practicing working memory tasks can result in generalizable benefits. To test this idea, they had some participants practice working memory tasks while other participants received no training. At the end of the study, those participants who practiced the working memory task (a dual-N back task, to be described later) improved on two measures of fluid intelligence (Gf) while the control group did not. This finding received a great deal of attention due to previously accepted assumptions that Gf is static and cannot be improved. Similarly, Dahlin, Nyberg, Bäckman, and Neely (2008) found evidence for transfer of training as long as the practiced task and the transfer task appeared to share common neural circuits. In this fMRI study, practicing one working memory tasked improved the performance of other tasks to the extent that the practiced task and the transfer tasked relied on the striatum. Based on these and other results, some scientists argue that training can target certain cognitive abilities and neural circuits, resulting in the improved performance of other tasks that depend on overlapping abilities and circuits.
Show less - Date Issued
- 2017-04-28
- Identifier
- FSU_libsubv1_scholarship_submission_1493396860
- Format
- Thesis
- Title
- La Sorella Dell'Ave: The Sister of the Ave Maria: A Translation of the Romanzi in Versi by Ludovica Ripa di Meana.
- Creator
-
Beasley, Jessica
- Abstract/Description
-
This work seeks to get a better grasp on translation in favor of doing justice to the book, La Sorella Dell'Ave by Ludovica Ripa di Meana. While the research surrounding translation theory is presented here, this project also included the first few chapters of the translation that this project worked on, with the help of Dr. Silvia Valisa. This research discusses ethical translation, feminist translation, translation decisions, and translation perceptions, specifically pertaining to western...
Show moreThis work seeks to get a better grasp on translation in favor of doing justice to the book, La Sorella Dell'Ave by Ludovica Ripa di Meana. While the research surrounding translation theory is presented here, this project also included the first few chapters of the translation that this project worked on, with the help of Dr. Silvia Valisa. This research discusses ethical translation, feminist translation, translation decisions, and translation perceptions, specifically pertaining to western culture.
Show less - Date Issued
- 2018-04-27
- Identifier
- FSU_libsubv1_scholarship_submission_1524812703_4f6e2250
- Format
- Thesis
- Title
- Investigating SREBP2 as a Potential Zika Virus Host Cofactor.
- Creator
-
Bell, Alexander Bell, Lee, Emily, Tang, Hengli
- Abstract/Description
-
Zika virus (ZIKV) is an Aedes mosquito transmitted flavivirus that has drawn global concern during the 2015-2016 epidemic in the Americas. The identification of host proteins and pathways may elucidate potential drug targets for antiviral therapy. Cholesterol synthesis has been shown to play an important role in the pathogenesis of several members of the flaviviridae family.16,18 Cholesterol biosynthesis occurs via the mevalonate pathway, and all participating enzymes are transcriptionally...
Show moreZika virus (ZIKV) is an Aedes mosquito transmitted flavivirus that has drawn global concern during the 2015-2016 epidemic in the Americas. The identification of host proteins and pathways may elucidate potential drug targets for antiviral therapy. Cholesterol synthesis has been shown to play an important role in the pathogenesis of several members of the flaviviridae family.16,18 Cholesterol biosynthesis occurs via the mevalonate pathway, and all participating enzymes are transcriptionally regulated by sterol regulatory-element binding proteins (SREBPs). 12,13 RNAi mediated expression knockdown of SREBP2 followed by NS1 FRET analysis reveals a 43% decrease in ZIKV infectivity in SNB-19 cells. Interestingly, RNAi mediated expression knockdown of individual genes that are transcriptionally activated by SREBP2 results in an increase of ZIKV infectivity. This suggests that the antiviral effect of SREBP2 knockdown is not solely mediated by depletion of mevalonate pathway products such as cholesterol. We hypothesize that the SREBP2 post-translational processing pathway contains pro-viral elements, and that ZIKV infectivity can be modulated by the activity of the pathway. Knockdown of SREBP2 does not induce spontaneous interferon beta (IFN-β) expression, but may increase IFN-β expression in the response to an infection. Overexpression of SREBP2 and SREBP1a result in increased transcription of mevalonate pathway enzymes, and increased ZIKV infection. SREBP1a’s enhancement of ZIKV infectivity indicates that SREBP2’s pro-viral role is likely mediated through the activity of it’s transcriptional targets. The specific mechanism of SREBP2’s interaction with ZIKV is not known, and is the subject of potential future plans for investigation.
Show less - Date Issued
- 2018-04-27
- Identifier
- FSU_libsubv1_scholarship_submission_1524861754_b7c9463e
- Format
- Thesis
- Title
- A Health Assessment of Historical Human Skeletal Remains from St. Augustine, Florida.
- Creator
-
Berger, Parker L.
- Abstract/Description
-
In recent decades, bioarchaeological technology and methodology used to analyze human skeletal remains and build biological profiles have drastically improved. Florida State University houses human skeletal remains from four sites in St. Augustine, Florida (8SA36-7, 8SA7-6, B28L1and B42L1) which were originally excavated and analyzed in the 1970s. This study uses current techniques to revisit previously examined skeletal material. Estimations of sex, age at death, and stature were reassessed,...
Show moreIn recent decades, bioarchaeological technology and methodology used to analyze human skeletal remains and build biological profiles have drastically improved. Florida State University houses human skeletal remains from four sites in St. Augustine, Florida (8SA36-7, 8SA7-6, B28L1and B42L1) which were originally excavated and analyzed in the 1970s. This study uses current techniques to revisit previously examined skeletal material. Estimations of sex, age at death, and stature were reassessed, and new body mass estimations were added. The current labeling of the material from three of the sites differed drastically from the labeling described in the previous analyses. This issue created challenges when trying to compare current finding to those of the previous assessments. However, this analysis yielded significantly different results from previous assessments when assigning sex, while concluding average age and stature estimates similar to previous assessments. Additionally, an updated and shareable digital catalogue, an inventory of materials, and collection of field notes and previous research reports were created. This project adds to established data and serves as a method of conservation of information associated with four St. Augustine archaeological sites.
Show less - Date Issued
- 2018-07-18
- Identifier
- FSU_libsubv1_scholarship_submission_1531941780_62896e46
- Format
- Thesis
- Title
- Reaction Kinetics of Lithium Electrodes with Solid Polymer Electrolytes.
- Creator
-
Berliner, Marc Dylan
- Abstract/Description
-
Solid polymer electrolytes have the potential to produce safe, effective alternative to liquid batteries. Creative, new approaches must be developed to determine reaction kinetics. An alternative method to cyclic voltammetry in rotating disk electrodes has been developed in solid polymer electrolytes. The novel pulsed voltammetry approach analysis was verified using a rotating disk electrode with a liquid ferri/ferrocyanide redox couple, and reasonable agreement for exchange current density...
Show moreSolid polymer electrolytes have the potential to produce safe, effective alternative to liquid batteries. Creative, new approaches must be developed to determine reaction kinetics. An alternative method to cyclic voltammetry in rotating disk electrodes has been developed in solid polymer electrolytes. The novel pulsed voltammetry approach analysis was verified using a rotating disk electrode with a liquid ferri/ferrocyanide redox couple, and reasonable agreement for exchange current density was found. Reaction kinetics of lithium electrodes were also studied in solid polymer electrolytes consisting of lithium bis(trifluoromethanesulfonyl)imide salt (LiTFSI) dissolved in polystyrene-b-poly(ethylene oxide) block copolymers (SEO). Symmetric Li cells were cycled with pulsed voltammetry to measure current at different salt concentrations and temperatures. Butler-Volmer kinetics for single-electron transfer, reversible reactions were applied to obtain exchange current density and the transfer coefficients. The plating reaction rate of lithium metal was found to be significantly greater than the stripping reaction rate. Reaction kinetics were found to be nearly independent of salt concentration, which suggests that they are dominated primarily by the nature of the lithium metal surface. The activation energy and pre-exponential factor for the plating of lithium metal was found to be a weak function of salt concentration.
Show less - Date Issued
- 2018-04-27
- Identifier
- FSU_libsubv1_scholarship_submission_1524850184_6a0b8089
- Format
- Thesis
- Title
- The Wrong Way to Parent.
- Creator
-
Bess-Lima, Tyler
- Abstract/Description
-
This is an argument against overprotective parenting. Those who do so are creating an environment that limits their children's psychological and moral growth and development. Overprotective parents control two very important aspects of their children's lives that are instrumental in their development. Parental control over the education and the social lives of their children creates children who do not have what is needed in order to make moral decisions. These children's capabilities or lack...
Show moreThis is an argument against overprotective parenting. Those who do so are creating an environment that limits their children's psychological and moral growth and development. Overprotective parents control two very important aspects of their children's lives that are instrumental in their development. Parental control over the education and the social lives of their children creates children who do not have what is needed in order to make moral decisions. These children's capabilities or lack there-of, as a result of this parenting, make them unable to make the most moral decisions possible. This argument bridges the gap between psychological and moral development of children and how parenting affects this. By taking psychological research and applying it to ethics and morals, one can come away from this argument understanding how overprotective parenting not only inhibits the psychological growth of children, but of their moral development as well.
Show less - Date Issued
- 2013
- Identifier
- FSU_migr_phi2630-0018
- Format
- Thesis
- Title
- The Categorical Imperative and Collegiate Society.
- Creator
-
Bess-Lima, Zachary
- Abstract/Description
-
This paper examines Greek Life and recreational drug use as two pillars of the collegiate society where college students are violating the 1st and 3rd formulations of Kant's categorical imperative. This is done by the analysis of the motivations behind such actions as hazing and social interactions in fraternities and sororities, and also how the act of using drugs is using your body as a mere means to achieve pleasure. This paper offers a new perspective to the collegiate landscape and...
Show moreThis paper examines Greek Life and recreational drug use as two pillars of the collegiate society where college students are violating the 1st and 3rd formulations of Kant's categorical imperative. This is done by the analysis of the motivations behind such actions as hazing and social interactions in fraternities and sororities, and also how the act of using drugs is using your body as a mere means to achieve pleasure. This paper offers a new perspective to the collegiate landscape and attempts to show how this can possibly be changed. This paper can possibly motivate a reformation of the two pillars and perhaps change the collegiate society for the better.
Show less - Date Issued
- 2013
- Identifier
- FSU_migr_phi2630-0017
- Format
- Thesis
- Title
- The role of compatibility in determining the costs and benefits of polyandry in the broadcast spawning urchin Lytechinus variegatus.
- Creator
-
Betters, Melissa
- Abstract/Description
-
When females mate with multiple males is a phenomenon known as polyandry. This is a strategy of sexual reproduction that presents both costs and benefits to female fitness. Mating with multiple males may maximize sperm availability and increase the likelihood of encountering a high-quality male. On the other hand, sperm may become overwhelming and result in polyspermy. With increasing mates, female could increase the risk of encountering low-quality males as well. The research presented in...
Show moreWhen females mate with multiple males is a phenomenon known as polyandry. This is a strategy of sexual reproduction that presents both costs and benefits to female fitness. Mating with multiple males may maximize sperm availability and increase the likelihood of encountering a high-quality male. On the other hand, sperm may become overwhelming and result in polyspermy. With increasing mates, female could increase the risk of encountering low-quality males as well. The research presented in this paper examined how these costs and benefits could potentially affect the fitness of individuals within a broadcast spawning population of marine urchins, Lytechinus variegatus. These organisms release their gametes into the water column, where fertilization occurs externally. This study attempted to examine how fertilization success was affected, if at all, by a female’s eggs encountering sperm from two different males in the water column rather than just one. Urchin color was also controlled for in order to see whether it may be used as a proxy for gametic compatibility between individuals. The data suggested that by pooling males together in the water column, rather than mating with one male at a time, the efficiency of fertilization increased. Therefore, it took less sperm to reach the same fertilization success when males were pooled together than when they were kept apart. Though assortative mating based on color was predicted, the data only showed evidence of this during the trials in which males were pooled together. When females were presented with a single male, male and female identity were instead the best predictors of compatibility.
Show less - Date Issued
- 2017-04-28
- Identifier
- FSU_libsubv1_scholarship_submission_1493414146
- Format
- Thesis
- Title
- Capillary Electrophoresis Separation of Formaldehyde Cross-Linked Aptamer-Protein Complexes.
- Creator
-
Duren, Joseph, Biochemistry
- Abstract/Description
-
An Immunoglobulin-E (IgE) DNA aptamer was covalently bound to IgE using a formaldehyde cross-linking method. The resulting complex was then separated from free aptamer by capillary electrophoresis (CE). The bound-to-free peak area ratios were preserved as a result of the cross-linking, which acted as a "freeze-frame" of the reaction in equilibrium. Elevated temperature occurring during CE separations can cause dissociation of non-covalently bound molecules and their targets, decreasing the...
Show moreAn Immunoglobulin-E (IgE) DNA aptamer was covalently bound to IgE using a formaldehyde cross-linking method. The resulting complex was then separated from free aptamer by capillary electrophoresis (CE). The bound-to-free peak area ratios were preserved as a result of the cross-linking, which acted as a "freeze-frame" of the reaction in equilibrium. Elevated temperature occurring during CE separations can cause dissociation of non-covalently bound molecules and their targets, decreasing the usefulness of the information obtained. Due to the formaldehyde cross-linking performed, the bound-to-free ratio was preserved over that of the uncross-linked control, which showed a decreased bound-to-free ratio upon heating. Additional trials were run on bovine serum albumin (BSA), anti-BSA, glucagon, and anti-glucagon using the formaldehyde cross-linking method. The results for this section were not conclusive due to difficulties in the separation and mechanistic issues with formaldehyde cross-linking. With the use of formaldehyde cross-linking, complexes that easily dissociate, under stringent separation conditions, can be covalently linked to prevent dissociation and allow for more sensitive analyses to be performed. Keywords:
Show less - Date Issued
- 2011
- Identifier
- FSU_migr_uhm-0012
- Format
- Thesis
- Title
- Phylogeographic Patterns in the Eastern Diamondback Rattlesnake (Crotalus adamanteus).
- Creator
-
Aronow, Karalyn, Biological Science
- Abstract/Description
-
The aim of this project was to analyze relationships among populations of Crotalus adamanteus (Eastern Diamondback Rattlesnake) and to compare phylogeographic patterns across different classes of genes. The objective was to determine the effect of selective pressures on the detection of barriers to gene flow. For example, venom genes are under strong positive selection and allow an analysis of the rate of allele exchange in a gene that directly affects the fitness of an individual. This...
Show moreThe aim of this project was to analyze relationships among populations of Crotalus adamanteus (Eastern Diamondback Rattlesnake) and to compare phylogeographic patterns across different classes of genes. The objective was to determine the effect of selective pressures on the detection of barriers to gene flow. For example, venom genes are under strong positive selection and allow an analysis of the rate of allele exchange in a gene that directly affects the fitness of an individual. This selection might drive alleles through phylogeographic barriers that limit the spread of neutral alleles. The relationship between nontoxin genes and fitness is not as clear and can be difficult to establish. However, most variation in these genes is neutral and determined by stochastic events. The type of selection on a gene will affect the phylogeographic pattern displayed. Prior phylogeographic studies have used either neutral markers (e.g., microsatellites) or genes not directly involved in specific adaptations (e.g., mitochondrial genes). One venom gene, two mitochondrial genes, one nuclear gene, and one pseudogene were compared for patterns of adaptation and the presence of geographic barriers to gene flow. This novel approach to studying gene flow could have an impact on defining taxonomic groups and conservation efforts, as well as serve as a guide for choosing appropriate genetic markers for other species. In the venom gene that was analyzed, it appeared the high level of selection led to alleles being driven across the barriers to gene flow of alleles under neutral selection.
Show less - Date Issued
- 2011
- Identifier
- FSU_migr_uhm-0002
- Format
- Thesis
- Title
- Amygdala Mechanisms Involved in Chemosensory Communication.
- Creator
-
Stroe, Ioana, Biological Science
- Abstract/Description
-
The amygdala is an almond-shaped region of the brain present in many vertebrates such as human, hamsters and mice. The amygdala is composed of several parts, of which the following are most relevant to my research: the basolateral amygdala (BLA) and the posterior medial amygdala (MeP). The BLA functions in learning and shows increased activation after the mouse has learned a behavior. The lateral paracapsular nuclei of the intercalated nucleus (ICNlpcn) project inhibitory neurons into the BLA...
Show moreThe amygdala is an almond-shaped region of the brain present in many vertebrates such as human, hamsters and mice. The amygdala is composed of several parts, of which the following are most relevant to my research: the basolateral amygdala (BLA) and the posterior medial amygdala (MeP). The BLA functions in learning and shows increased activation after the mouse has learned a behavior. The lateral paracapsular nuclei of the intercalated nucleus (ICNlpcn) project inhibitory neurons into the BLA and mediate its activity. Dopamine has been shown to inhibit the lpcn group and therefore relieve inhibition of the BLA (disinhibition). Dopamine also acts directly in the BLA to increase activation. Therefore, if a mouse is conditioned to prefer a particular odor (in this case steer urine), dopamine injection will cause an increase in BLA activation after learning due to both disinhibition and direct excitatory response. The medial posterior amygdala in the mouse mostly responds to olfactory stimuli from the same species (conspecific stimuli). The MeP is also thought to be under control of another ICN group, the caudal ICN (ICNc). If the same inhibitory relationship is seen between ICNc and MeP, the dopamine injection may cause disinhibition in the MeP and therefore the conditioned steer urine stimulus may show increased activation in the MeP despite being a stimulus from another species (heterospecific stimulus). My experiment tests whether the ICNc has inhibitory control over the MeP, and whether dopamine will cause inhibition in the ICNc and therefore cause disinhibition in the MeP.
Show less - Date Issued
- 2011
- Identifier
- FSU_migr_uhm-0040
- Format
- Thesis
- Title
- The Cost of Safety: Negotiating the Trade-Off between Safety and Growth.
- Creator
-
Matechik, Christopher, Biological Science
- Abstract/Description
-
To avoid the threat of predation, prey can alter their behavior such as moving from habitats where predation risk is high to those where it is low. However, such behaviors may involve a tradeoff if the low risk habitats are suboptimal feeding locations for the prey compared to the high risk ones. In seagrass, pinfish (Lagodon rhomboides) often move from low- to high-blade density patches in the presence of the predatory Gulf flounder (Paralichthys albigutta). However, restricted movements in...
Show moreTo avoid the threat of predation, prey can alter their behavior such as moving from habitats where predation risk is high to those where it is low. However, such behaviors may involve a tradeoff if the low risk habitats are suboptimal feeding locations for the prey compared to the high risk ones. In seagrass, pinfish (Lagodon rhomboides) often move from low- to high-blade density patches in the presence of the predatory Gulf flounder (Paralichthys albigutta). However, restricted movements in high-blade habitats may lead to reduced foraging efficiency and growth rates. I used a field experiment that ran for 28 days to examine this potential tradeoff between survival and growth. Neither predator presence nor seagrass blade density affected survival or growth in pinfish. However, after accounting for the loss of experimental replicates at lower grass-blade densities, the change in pinfish biomass was greater in the predator-present treatment compared to the controls, suggesting higher mortality, increased vigilance or both when flounder were present. Unfortunately, loss of experimental replicates and high variance in the measured responses hindered my ability to address the potential tradeoff, but suggestive results support further examination.
Show less - Date Issued
- 2011
- Identifier
- FSU_migr_uhm-0025
- Format
- Thesis
- Title
- Characterization of Maize Transgene-Reactivated Mutants.
- Creator
-
Mills, Erica, Biological Science
- Abstract/Description
-
Epigenetic gene regulation results from heritable changes in chromatin structure, DNA methylation, and/or histone modifications associated with different expression levels for endogenous genes and transgenes. The silent b1 transgene (Btg-s) can be used to study epigenetic gene regulation in maize. A forward genetics screen based on the reactivation of Btg-s was conducted using EMS mutagenized maize. Through this screen, several mutants reactivating the transgene, designated transgene...
Show moreEpigenetic gene regulation results from heritable changes in chromatin structure, DNA methylation, and/or histone modifications associated with different expression levels for endogenous genes and transgenes. The silent b1 transgene (Btg-s) can be used to study epigenetic gene regulation in maize. A forward genetics screen based on the reactivation of Btg-s was conducted using EMS mutagenized maize. Through this screen, several mutants reactivating the transgene, designated transgene reactivated (tgr), were identified. Published work demonstrates that mutations affecting proteins involved in the RNA-dependent DNA methylation pathway cause transgene reactivation. Such mutations result in characteristic phenotypes, such as a loss of DNA methylation, loss of siRNA accumulation, and loss of transcriptional silencing. To investigate Btg promoter methylation in the tgr mutants, bisulfite sequencing was performed on plants with silenced and reactivated transgenes from four lines of tgr mutants (tgr1-3,11). Results show that in three mutant lines, the promoter region had higher methylation levels in silenced plants compared to reactivated plants, while another mutant had similar levels of methylation in both phenotypes. For tgr2, siRNA levels were assayed and transcript abundance was evaluated for genes encoding proteins possibly involved in the methylation pathway. It was found that tgr2 showed no loss of siRNA production and no difference in transcript levels were observed between silenced and reactivated individuals for the genes evaluated. This work aided in characterizing the tgr mutants which are potentially strong tools for understanding epigenetic regulation of transgenes.
Show less - Date Issued
- 2011
- Identifier
- FSU_migr_uhm-0028
- Format
- Thesis