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Conservation of Dehydration Stress Memories Between Abiotic Stresses in Zea mays and the Characterization of Drought-Responsive Morphology in mop1-1 Mutants

Title: Conservation of Dehydration Stress Memories Between Abiotic Stresses in Zea mays and the Characterization of Drought-Responsive Morphology in mop1-1 Mutants.
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Name(s): Lemert, Phebe Harriet, author
Type of Resource: text
Genre: Text
Bachelor Thesis
Date Issued: 2020-04-24
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Plants are subjected to a wide range of environmental stressors that require rapid transcriptional responses. In maize (Zea mays), the pathways mediating abiotic stress response are important to characterize as they are most likely interconnected with one another. The phytohormone abscisic acid (ABA) is an important signaling molecule in abiotic stress, accumulating in plant cells to trigger physiological responses. ABA-responsive transcription factors (TFs) and target genes organize themselves in a hierarchical regulatory network. Previous studies have also demonstrated the existence of “dehydration stress memories,” in which transcriptional responses to short-term water scarcity can be altered to produce increased or decreased gene expression upon future stresses. MEDIATOR OF PARAMUTATION 1 (MOP1) and 3 (MOP3) are required for the normal function of the RNA-dependent DNA methylation pathway, which regulates the transcriptional silencing of specific DNA sequences. These silencing patterns can be stably inherited over multiple generations. RdDM has also been implicated in the regulation of abiotic responses, particularly with drought and ABA-response. We explored dehydration stress memories previously reported in the literature to explore the conservation of drought and ABA-stressed plants. Publicly available RNA-seq data for B73 and RdDM-related lines were run down a differential gene expression pipeline, comparing experimental groups in pairs to determine shared differentially expressed genes (DEGs). DEGs were then filtered according to a list of known 2,062 dehydration stress memory and nonmemory genes, then organized according to gene name on an ABA-responsive TF hierarchy. A total of 196 dehydration stress memories and nonmemories were differentially expressed comparisons between drought, dehydration, and ABA stresses. 35 dehydration stress memories and nonmemories were identified as ABA-responsive transcription factors and target genes. These results show that dehydration stress memories are differentially expressed across other abiotic stress conditions and may play a larger role in transcription factor regulatory networks.We also provide a morphological guide to how the loss of MOP1 can affect drought tolerance. Loss of MOP1 can lead to an overall decrease in drought tolerance related to growth, particularly internodal length and stages of vegetative development. This provides further support that MOP1 is a useful candidate to understand epigenetic mechanisms mediating drought and the maintenance of dehydration stress memories with loss of RdDM.
Identifier: FSU_libsubv1_scholarship_submission_1588008687_cb4b5c41 (IID)
Keywords: epigenetics, corn, plant biology, genetics, drought, dehydration, RNA-directed DNA methylation, maize, ABA, abscisic acid,
Publication Note: A Thesis submitted to the Department of Biological Science in partial fulfillment of the requirements for the degree of Honors in the Major.
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1588008687_cb4b5c41
Host Institution: FSU

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Lemert, P. H. (2020). Conservation of Dehydration Stress Memories Between Abiotic Stresses in Zea mays and the Characterization of Drought-Responsive Morphology in mop1-1 Mutants. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1588008687_cb4b5c41