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Proteasome Subunit Alpha 1 Overexpression Preferentially Drives Canonical Proteasome Biogenesis And Enhances Stress Tolerance In Yeast

Title: Proteasome Subunit Alpha 1 Overexpression Preferentially Drives Canonical Proteasome Biogenesis And Enhances Stress Tolerance In Yeast.
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Name(s): Howell, Lauren A., author
Peterson, Anna K., author
Tomko, Robert J., author
Type of Resource: text
Genre: Journal Article
Text
Journal Article
Date Issued: 2019-08-27
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The 26S proteasome conducts the majority of regulated protein catabolism in eukaryotes. At the heart of the proteasome is the barrel-shaped 20S core particle (CP), which contains two beta-rings sandwiched between two alpha-rings. Whereas canonical CPs contain alpha-rings with seven subunits arranged alpha 1-alpha 7, a non-canonical CP in which a second copy of the alpha 4 subunit replaces the alpha 3 subunit occurs in both yeast and humans. The mechanisms that control canonical versus non-canonical CP biogenesis remain poorly understood. Here, we have repurposed a split-protein reporter to identify genes that can enhance canonical proteasome assembly in mutant yeast producing non-canonical alpha 4-alpha 4 CPs. We identified the proteasome subunit alpha 1 as an enhancer of alpha 3 incorporation, and find that elevating alpha 1 protein levels preferentially drives canonical CP assembly under conditions that normally favor alpha 4-alpha 4 CP formation. Further, we demonstrate that alpha 1 is stoichiometrically limiting for alpha-ring assembly, and that enhancing alpha 1 levels is sufficient to increase proteasome abundance and enhance stress tolerance in yeast. Together, our data indicate that the abundance of alpha 1 exerts multiple impacts on proteasome assembly and composition, and we propose that the limited alpha 1 levels observed in yeast may prime cells for alternative proteasome assembly following environmental stimuli.
Identifier: FSU_libsubv1_wos_000482709400029 (IID), 10.1038/s41598-019-48889-5 (DOI)
Keywords: expression, selection, genes, completion, 20s proteasome, 26s proteasome, chaperones, substrate
Publication Note: The publisher's version of record is available at https://doi.org/10.1038/s41598-019-48889-5
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000482709400029
Host Institution: FSU
Is Part Of: Scientific Reports.
2045-2322
Issue: vol. 9

Choose the citation style.
Howell, L. A., Peterson, A. K., & Tomko, R. J. (2019). Proteasome Subunit Alpha 1 Overexpression Preferentially Drives Canonical Proteasome Biogenesis And Enhances Stress Tolerance In Yeast. Scientific Reports. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000482709400029