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Cell Growth and Differentiation of P19 Cells In a 3D Bioprinted Scaffold

Title: Cell Growth and Differentiation of P19 Cells In a 3D Bioprinted Scaffold.
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Name(s): Drulias, Nicholas R., author
Type of Resource: text
Genre: Text
Bachelor Thesis
Date Issued: 2019-04-26
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Sodium alginate (A) and gelatin (G) based hydrogels are used extensively for biomedical applications to mimic the tissues and scaffolds in which cells are placed to differentiate. The rheological properties of the underlying hydrogel play an important part in cell encapsulation and differentiation and in one’s ability to process the gels into useful structures. Of particular interest is 3D printing hydrogels into scaffolds which mimic the external matrix of a certain cell line and still maintain cell viability. In this work we rheologically characterize hydrogels of alginate and gelatin with aim of 3D printing scaffolds for P19 muscle line. Pre and post chemical crosslinking rheological experiments were conducted on hydrogels to identify properties that effect print ability and print uniformity. Hydrogels were chemically crosslinked using CaCl2 and it was found that 2.5%(w/w) gelatin – 7.5%(w/w) alginate experienced the highest level of crosslinking. In addition, this composition experienced the highest level of printability and showed the most promise for cell culturing P19 in a 3D printed bio scaffold.
Identifier: FSU_libsubv1_scholarship_submission_1556303687_ed3a1947 (IID)
Keywords: Rheology, P19, Bioink, 3D printing, bioprinting
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1556303687_ed3a1947
Host Institution: FSU

Choose the citation style.
Drulias, N. R. (2019). Cell Growth and Differentiation of P19 Cells In a 3D Bioprinted Scaffold. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1556303687_ed3a1947