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Effects of conjugated linoleic acid/n-3 and resistance training on muscle quality and expression of atrophy-related ubiquitin ligases in middle-aged mice with high-fat dietinduced obesity.

Title: Effects of conjugated linoleic acid/n-3 and resistance training on muscle quality and expression of atrophy-related ubiquitin ligases in middle-aged mice with high-fat dietinduced obesity.
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Name(s): Oh, Seung-Lyul, author
Lee, Sang-Rok, author
Kim, Jeong-Su, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-09-30
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: To investigate the effects of conjugated linoleic acid (CLA)/n-3 supplements and resistance exercise training (RT) for 20 weeks on muscle quality and genes related to protein synthesis/degradation in middle-aged mice with high-fat diet (HFD)-induced obesity. Nine-month-old C57BL/6 male mice were randomly assigned to five groups: 1) normal diet (C), 2) high-fat diet (H), 3) H+RT (HRT), 4) H+CLA/n-3 (H-CN), and 5) H+RT+CLA/n-3 (H-RTCN). HFD groups were given a diet containing 60% fat for 20 weeks, and exercised groups underwent progressive RT using weighted ladder climbing. The CLA/n-3 mixed diet contained 1% CLA and 1% n-3. Grip strength was assessed, and triceps were removed. RT-PCR was used to analyze transcript levels. Grip strength of the H group was significantly lower than that of the C group; however, those in the H-CN, H-RT, and H-RTN groups were significantly greater than that in the H group. However, the muscle quality was significantly greater only in the H-RT group compared with the H and H-CN groups. Akt expression decreased in the H-CN, H-RT, and H-RTCN groups compared with those in the C and H groups, whereas mammalian target of rapamycin expression increased in the H, H-CN, H-RT, and H-RTCN groups compared with that in the C group. However, atrogin1 was significantly downregulated in the H-RTCN group compared with that in the H and H-CN groups, and MuRF1 expression was also decreased in the H-RT and H-RTCN groups. Interestingly, atrogin1 and MuRF1 were downregulated in the H-RTCN group compared with that in the H-CN group. HFD-mediated gene expression involved in protein degradation was attenuated following 20-week RT with CLA/n-3. Furthermore, RT with or without CLA/n-3 improved grip strength and muscle quality in middle-aged mice during HFD. Therefore, RT with CLA/n-3 during HFD may improve muscle strength and quality by suppressing protein degradation.
Identifier: FSU_pmch_29036761 (IID), 10.20463/jenb.2017.0028 (DOI), PMC5643205 (PMCID), 29036761 (RID), 29036761 (EID), JENB_2017_v21n3 (PII)
Keywords: Conjugated linoleic acid (CLA), Muscle atrophy, Muscle quality, Omega-3 polyunsaturated fatty acid (n-3 PUFAs), Resistance training
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643205.
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_29036761
Host Institution: FSU
Is Part Of: Journal of exercise nutrition & biochemistry.
2233-6834
Issue: iss. 3, vol. 21

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Oh, S. -L., Lee, S. -R., & Kim, J. -S. (2017). Effects of conjugated linoleic acid/n-3 and resistance training on muscle quality and expression of atrophy-related ubiquitin ligases in middle-aged mice with high-fat dietinduced obesity. Journal Of Exercise Nutrition & Biochemistry. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_29036761