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3'-UTR and microRNA-24 regulate circadian rhythms by repressing PERIOD2 protein accumulation.

Title: 3'-UTR and microRNA-24 regulate circadian rhythms by repressing PERIOD2 protein accumulation.
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Name(s): Yoo, Seung-Hee, author
Kojima, Shihoko, author
Shimomura, Kazuhiro, author
Koike, Nobuya, author
Buhr, Ethan D, author
Furukawa, Tadashi, author
Ko, Caroline H, author
Gloston, Gabrielle, author
Ayoub, Christopher, author
Nohara, Kazunari, author
Reyes, Bryan A, author
Tsuchiya, Yoshiki, author
Yoo, Ook-Joon, author
Yagita, Kazuhiro, author
Lee, Choogon, author
Chen, Zheng, author
Yamazaki, Shin, author
Green, Carla B, author
Takahashi, Joseph S, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-10-17
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: We previously created two PER2::LUCIFERASE (PER2::LUC) circadian reporter knockin mice that differ only in the 3'-UTR region: , which retains the endogenous 3'-UTR and , where the endogenous 3'-UTR was replaced by an SV40 late poly(A) signal. To delineate the in vivo functions of 3'-UTR, we analyzed circadian rhythms of mice. Interestingly, mice displayed more than threefold stronger amplitude in bioluminescence rhythms than mice, and also exhibited lengthened free-running periods (∼24.0 h), greater phase delays following light pulse, and enhanced temperature compensation relative to Analysis of the 3'-UTR sequence revealed that miR-24, and to a lesser degree miR-30, suppressed PER2 protein translation, and the reversal of this inhibition in augmented PER2::LUC protein level and oscillatory amplitude. Interestingly, mRNA and protein oscillatory amplitude as well as CRY1 protein oscillation were increased in mice, suggesting rhythmic overexpression of PER2 enhances expression of and other core clock genes. Together, these studies provide important mechanistic insights into the regulatory roles of 3'-UTR, miR-24, and PER2 in expression and core clock function.
Identifier: FSU_pmch_28973913 (IID), 10.1073/pnas.1706611114 (DOI), PMC5651750 (PMCID), 28973913 (RID), 28973913 (EID), 1706611114 (PII)
Keywords: 3′-UTR regulation, Per2 gene, Circadian, MiR-24, MicroRNA
Grant Number: R01 GM114424, R01 GM112991, R01 GM111387, , P50 MH074924, R01 AG045828
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651750.
Subject(s): 3' Untranslated Regions
Animals
Circadian Clocks/genetics
Circadian Rhythm/physiology
Gene Expression Regulation
Gene Knock-In Techniques
Luciferases/genetics
Mice, Inbred C57BL
Mice, Transgenic
MicroRNAs/genetics
Period Circadian Proteins/genetics
Period Circadian Proteins/metabolism
Protein Biosynthesis
Temperature
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28973913
Owner Institution: FSU
Is Part Of: Proceedings of the National Academy of Sciences of the United States of America.
1091-6490
Issue: iss. 42, vol. 114

Choose the citation style.
Yoo, S. -H., Kojima, S., Shimomura, K., Koike, N., Buhr, E. D., Furukawa, T., … Takahashi, J. S. (2017). 3'-UTR and microRNA-24 regulate circadian rhythms by repressing PERIOD2 protein accumulation. Proceedings Of The National Academy Of Sciences Of The United States Of America. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28973913