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Metabolic and spatio-taxonomic response of uncultivated seafloor bacteria following the Deepwater Horizon oil spill.

Title: Metabolic and spatio-taxonomic response of uncultivated seafloor bacteria following the Deepwater Horizon oil spill.
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Name(s): Handley, K M, author
Piceno, Y M, author
Hu, P, author
Tom, L M, author
Mason, O U, author
Andersen, G L, author
Jansson, J K, author
Gilbert, J A, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-11-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The release of 700 million liters of oil into the Gulf of Mexico over a few months in 2010 produced dramatic changes in the microbial ecology of the water and sediment. Here, we reconstructed the genomes of 57 widespread uncultivated bacteria from post-spill deep-sea sediments, and recovered their gene expression pattern across the seafloor. These genomes comprised a common collection of bacteria that were enriched in heavily affected sediments around the wellhead. Although rare in distal sediments, some members were still detectable at sites up to 60 km away. Many of these genomes exhibited phylogenetic clustering indicative of common trait selection by the environment, and within half we identified 264 genes associated with hydrocarbon degradation. Alkane degradation ability was near ubiquitous among candidate hydrocarbon degraders, whereas just three harbored elaborate gene inventories for the degradation of alkanes and aromatic and polycyclic aromatic hydrocarbons (PAHs). Differential gene expression profiles revealed a spill-promoted microbial sulfur cycle alongside gene upregulation associated with PAH degradation. Gene expression associated with alkane degradation was widespread, although active alkane degrader identities changed along the pollution gradient. Analyses suggest that a broad metabolic capacity to respond to oil inputs exists across a large array of usually rare indigenous deep-sea bacteria.
Identifier: FSU_pmch_28777379 (IID), 10.1038/ismej.2017.110 (DOI), PMC5649166 (PMCID), 28777379 (RID), 28777379 (EID), ismej2017110 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5649166.
Subject(s): Alkanes/analysis
Alkanes/metabolism
Bacteria/classification
Bacteria/genetics
Bacteria/isolation & purification
Bacteria/metabolism
Bacterial Proteins/genetics
Bacterial Proteins/metabolism
Biodegradation, Environmental
Geologic Sediments/analysis
Geologic Sediments/microbiology
Gulf of Mexico
Petroleum/metabolism
Petroleum Pollution/analysis
Phylogeny
Polycyclic Aromatic Hydrocarbons/analysis
Polycyclic Aromatic Hydrocarbons/metabolism
Seawater/analysis
Seawater/microbiology
Water Pollutants, Chemical/analysis
Water Pollutants, Chemical/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28777379
Owner Institution: FSU
Is Part Of: The ISME journal.
1751-7370
Issue: iss. 11, vol. 11

Choose the citation style.
Handley, K. M., Piceno, Y. M., Hu, P., Tom, L. M., Mason, O. U., Andersen, G. L., … Gilbert, J. A. (2017). Metabolic and spatio-taxonomic response of uncultivated seafloor bacteria following the Deepwater Horizon oil spill. The Isme Journal. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28777379