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Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein.

Title: Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein.
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Name(s): Sakano, Hitomi, author
Zorio, Diego A R, author
Wang, Xiaoyu, author
Ting, Ying S, author
Noble, William S, author
MacCoss, Michael J, author
Rubel, Edwin W, author
Wang, Yuan, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-10-15
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The avian nucleus laminaris (NL) is a brainstem nucleus necessary for binaural processing, analogous in structure and function to the mammalian medial superior olive. In chickens (Gallus gallus), NL is a well-studied model system for activity-dependent neural plasticity. Its neurons have bipolar extension of dendrites, which receive segregated inputs from two ears and display rapid and compartment-specific reorganization in response to unilateral changes in auditory input. More recently, fragile X mental retardation protein (FMRP), an RNA-binding protein that regulates local protein translation, has been shown to be enriched in NL dendrites, suggesting its potential role in the structural dynamics of these dendrites. To explore the molecular role of FMRP in this nucleus, we performed proteomic analysis of NL, using micro laser capture and liquid chromatography tandem mass spectrometry. We identified 657 proteins, greatly represented in pathways involved in mitochondria, translation and metabolism, consistent with high levels of activity of NL neurons. Of these, 94 are potential FMRP targets, by comparative analysis with previously proposed FMRP targets in mammals. These proteins are enriched in pathways involved in cellular growth, cellular trafficking and transmembrane transport. Immunocytochemistry verified the dendritic localization of several proteins in NL. Furthermore, we confirmed the direct interaction of FMRP with one candidate, RhoC, by in vitro RNA binding assays. In summary, we provide a database of highly expressed proteins in NL and in particular a list of potential FMRP targets, with the goal of facilitating molecular characterization of FMRP signaling in future studies.
Identifier: FSU_pmch_28685837 (IID), 10.1002/cne.24281 (DOI), PMC5564178 (PMCID), 28685837 (RID), 28685837 (EID)
Keywords: RNA binding protein, RRID: AB_10615780, RRID: AB_1859928, RRID: AB_2155806, RRID: AB_2277755, RRID: AB_2620155, RRID: AB_297884, RRID: AB_309663, RRID: AB_357520, RRID: AB_776174, RRID: AB_94856, RhoC, Autism spectrum disorders, Cytoskeletal proteins, Dendritic plasticity, Gene ontology
Grant Number: P41 GM103533, T32 DC000018, R01 GM121818, R01 DC003829, R01 DC013074
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564178.
Subject(s): Animals
Avian Proteins/metabolism
Blotting, Western
Brain Stem/cytology
Brain Stem/metabolism
Chickens
Chromatography, Liquid
Dendrites/metabolism
Dermoscopy
Electrophoresis
Escherichia coli
Fragile X Mental Retardation Protein/metabolism
Immunohistochemistry
Laser Capture Microdissection
Microscopy, Confocal
Proteome
Proteomics
Recombinant Proteins/metabolism
Tandem Mass Spectrometry
rhoC GTP-Binding Protein/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28685837
Owner Institution: FSU
Is Part Of: The Journal of comparative neurology.
1096-9861
Issue: iss. 15, vol. 525

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Sakano, H., Zorio, D. A. R., Wang, X., Ting, Y. S., Noble, W. S., MacCoss, M. J., … Wang, Y. (2017). Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein. The Journal Of Comparative Neurology. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28685837