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Cell-cycle dynamics of chromosomal organization at single-cell resolution.

Title: Cell-cycle dynamics of chromosomal organization at single-cell resolution.
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Name(s): Nagano, Takashi, author
Lubling, Yaniv, author
Várnai, Csilla, author
Dudley, Carmel, author
Leung, Wing, author
Baran, Yael, author
Mendelson Cohen, Netta, author
Wingett, Steven, author
Fraser, Peter, author
Tanay, Amos, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-07-05
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Chromosomes in proliferating metazoan cells undergo marked structural metamorphoses every cell cycle, alternating between highly condensed mitotic structures that facilitate chromosome segregation, and decondensed interphase structures that accommodate transcription, gene silencing and DNA replication. Here we use single-cell Hi-C (high-resolution chromosome conformation capture) analysis to study chromosome conformations in thousands of individual cells, and discover a continuum of cis-interaction profiles that finely position individual cells along the cell cycle. We show that chromosomal compartments, topological-associated domains (TADs), contact insulation and long-range loops, all defined by bulk Hi-C maps, are governed by distinct cell-cycle dynamics. In particular, DNA replication correlates with a build-up of compartments and a reduction in TAD insulation, while loops are generally stable from G1 to S and G2 phase. Whole-genome three-dimensional structural models reveal a radial architecture of chromosomal compartments with distinct epigenomic signatures. Our single-cell data therefore allow re-interpretation of chromosome conformation maps through the prism of the cell cycle.
Identifier: FSU_pmch_28682332 (IID), 10.1038/nature23001 (DOI), PMC5567812 (PMCID), 28682332 (RID), 28682332 (EID), nature23001 (PII)
Grant Number: U01 HL129971
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5567812.
Subject(s): Animals
Cell Compartmentation
Cell Cycle/genetics
Cell Cycle/physiology
Chromosomes, Mammalian/chemistry
Chromosomes, Mammalian/genetics
Chromosomes, Mammalian/metabolism
Epigenesis, Genetic
Haploidy
Imaging, Three-Dimensional
Mice
Mouse Embryonic Stem Cells/cytology
Reproducibility of Results
Single-Cell Analysis/methods
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28682332
Host Institution: FSU
Is Part Of: Nature.
1476-4687
Issue: iss. 7661, vol. 547

Choose the citation style.
Nagano, T., Lubling, Y., Várnai, C., Dudley, C., Leung, W., Baran, Y., … Tanay, A. (2017). Cell-cycle dynamics of chromosomal organization at single-cell resolution. Nature. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28682332