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clearance of spindle assembly checkpoint proteins from kinetochores

Title: The clearance of spindle assembly checkpoint proteins from kinetochores.
Name(s): Wang, Yanchang, author
Type of Resource: text
Genre: Text
Journal Article
Date Issued: 2016
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The spindle assembly checkpoint (SAC) monitors chromosome attachment defects, and the assembly of SAC proteins at kinetochores is essential for its activation, but the SAC disassembly process remains unknown. We found that deletion of a 14-3-3 protein, Bmh1, or hyperactivation of Cdc14 early anaphase release (FEAR) allows premature SAC silencing in budding yeast, which depends on a kinetochore protein Fin1 that forms a complex with protein phosphatase PP1. Previous works suggest that FEAR-dependent Fin1 dephosphorylation promotes Bmh1-Fin1 dissociation, which enables kinetochore recruitment of Fin1-PP1. We found persistent kinetochore association of SAC protein Bub1 in fin1D mutants after anaphase entry. Therefore, we revealed a mechanism that clears SAC proteins from kinetochores. After anaphase entry, FEAR activation promotes kinetochore enrichment of Fin1-PP1, resulting in SAC disassembly at kinetochores. This mechanism is required for efficient SAC silencing after SAC is challenged, and untimely Fin1-kinetochore association causes premature SAC silencing and chromosome missegregation.
Identifier: FSU_libsubv1_scholarship_submission_1551394886_dc4f9b78 (IID), 10.1016/j.celrep.2016.01.007 (DOI)
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Use and Reproduction: Creative Commons Attribution (CC BY 4.0)
Owner Institution: FSU
Is Part Of: Cell Reports.

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Wang, Y. (2016). The clearance of spindle assembly checkpoint proteins from kinetochores. Cell Reports. Retrieved from