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Cleavage of the SUN-domain protein Mps3 at its N-terminus regulates centrosome disjunction in budding yeast meiosis.

Title: Cleavage of the SUN-domain protein Mps3 at its N-terminus regulates centrosome disjunction in budding yeast meiosis.
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Name(s): Li, Ping, author
Jin, Hui, author
Koch, Bailey A, author
Abblett, Rebecca L, author
Han, Xuemei, author
Yates, John R, author
Yu, Hong-Guo, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-06-13
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Centrosomes organize microtubules and are essential for spindle formation and chromosome segregation during cell division. Duplicated centrosomes are physically linked, but how this linkage is dissolved remains unclear. Yeast centrosomes are tethered by a nuclear-envelope-attached structure called the half-bridge, whose components have mammalian homologues. We report here that cleavage of the half-bridge protein Mps3 promotes accurate centrosome disjunction in budding yeast. Mps3 is a single-pass SUN-domain protein anchored at the inner nuclear membrane and concentrated at the nuclear side of the half-bridge. Using the unique feature in yeast meiosis that centrosomes are linked for hours before their separation, we have revealed that Mps3 is cleaved at its nucleus-localized N-terminal domain, the process of which is regulated by its phosphorylation at serine 70. Cleavage of Mps3 takes place at the yeast centrosome and requires proteasome activity. We show that noncleavable Mps3 (Mps3-nc) inhibits centrosome separation during yeast meiosis. In addition, overexpression of mps3-nc in vegetative yeast cells also inhibits centrosome separation and is lethal. Our findings provide a genetic mechanism for the regulation of SUN-domain protein-mediated activities, including centrosome separation, by irreversible protein cleavage at the nuclear periphery.
Identifier: FSU_pmch_28609436 (IID), 10.1371/journal.pgen.1006830 (DOI), PMC5487077 (PMCID), 28609436 (RID), 28609436 (EID), PGENETICS-D-17-00030 (PII)
Grant Number: R01 GM117102
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487077.
Subject(s): Centrosome/metabolism
Chromosome Segregation/genetics
Gene Expression Regulation, Fungal
Meiosis/genetics
Membrane Proteins/genetics
Microtubules/genetics
Nuclear Envelope/genetics
Nuclear Proteins/genetics
Protein Domains/genetics
Saccharomyces cerevisiae/genetics
Saccharomyces cerevisiae Proteins/genetics
Spindle Apparatus/genetics
Telomere/genetics
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28609436
Host Institution: FSU
Is Part Of: PLoS genetics.
1553-7404
Issue: iss. 6, vol. 13

Choose the citation style.
Li, P., Jin, H., Koch, B. A., Abblett, R. L., Han, X., Yates, J. R., & Yu, H. -G. (2017). Cleavage of the SUN-domain protein Mps3 at its N-terminus regulates centrosome disjunction in budding yeast meiosis. Plos Genetics. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28609436