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MicroRNA-211 Regulates Oxidative Phosphorylation and Energy Metabolism in Human Vitiligo.

Title: MicroRNA-211 Regulates Oxidative Phosphorylation and Energy Metabolism in Human Vitiligo.
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Name(s): Sahoo, Anupama, author
Lee, Bongyong, author
Boniface, Katia, author
Seneschal, Julien, author
Sahoo, Sanjaya K, author
Seki, Tatsuya, author
Wang, Chunyan, author
Das, Soumen, author
Han, Xianlin, author
Steppie, Michael, author
Seal, Sudipta, author
Taieb, Alain, author
Perera, Ranjan J, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-09-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Vitiligo is a common chronic skin disorder characterized by loss of epidermal melanocytes and progressive depigmentation. Vitiligo has complex immune, genetic, environmental, and biochemical causes, but the exact molecular mechanisms of vitiligo development and progression, particularly those related to metabolic control, are poorly understood. In this study we characterized the human vitiligo cell line PIG3V and the normal human melanocyte line HEM-l by RNA sequencing, targeted metabolomics, and shotgun lipidomics. Melanocyte-enriched microRNA-211, a known metabolic switch in nonpigmented melanoma cells, was severely down-regulated in vitiligo cell line PIG3V and skin biopsy samples from vitiligo patients, whereas its predicted targets PPARGC1A, RRM2, and TAOK1 were reciprocally up-regulated. microRNA-211 binds to PGC1-α 3' untranslated region locus and represses it. Although mitochondrial numbers were constant, mitochondrial complexes I, II, and IV and respiratory responses were defective in vitiligo cells. Nanoparticle-coated microRNA-211 partially augmented the oxygen consumption rate in PIG3V cells. The lower oxygen consumption rate, changes in lipid and metabolite profiles, and increased reactive oxygen species production observed in vitiligo cells appear to be partly due to abnormal regulation of microRNA-211 and its target genes. These genes represent potential biomarkers and therapeutic targets in human vitiligo.
Identifier: FSU_pmch_28502800 (IID), 10.1016/j.jid.2017.04.025 (DOI), PMC6233982 (PMCID), 28502800 (RID), 28502800 (EID), S0022-202X(17)31491-4 (PII)
Grant Number: P30 CA030199, R03 CA165184
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233982.
Subject(s): Cells, Cultured
Energy Metabolism/genetics
Female
Humans
Keratinocytes/metabolism
Male
Melanocytes/metabolism
MicroRNAs/genetics
Oxidative Phosphorylation
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha/genetics
Reactive Oxygen Species/metabolism
Real-Time Polymerase Chain Reaction/methods
Sensitivity and Specificity
Vitiligo/genetics
Vitiligo/physiopathology
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28502800
Owner Institution: FSU
Is Part Of: The Journal of investigative dermatology.
1523-1747
Issue: iss. 9, vol. 137

Choose the citation style.
Sahoo, A., Lee, B., Boniface, K., Seneschal, J., Sahoo, S. K., Seki, T., … Perera, R. J. (2017). MicroRNA-211 Regulates Oxidative Phosphorylation and Energy Metabolism in Human Vitiligo. The Journal Of Investigative Dermatology. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28502800