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Cas6 processes tight and relaxed repeat RNA via multiple mechanisms

Title: Cas6 processes tight and relaxed repeat RNA via multiple mechanisms: A hypothesis.
Name(s): Sefcikova, Jana, author
Roth, Mitchell, author
Yu, Ge, author
Li, Hong, author
Type of Resource: text
Genre: Journal Article
Date Issued: 2017-06-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: RNA molecules are flexible yet foldable. Proteins must cope with this structural duality when forming biologically active complexes with RNA. Recent studies of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs)-mediated RNA immunity illustrate some remarkable mechanisms with which proteins interact with RNA. Currently known structures of CRISPR-Cas6 endoribonucleases bound with RNA suggest a conserved protein recognition mechanism mediated by RNA stem-loops. However, a survey of CRISPR RNA reveals that many repeats either lack a productive stem-loop (Relaxed) or possess stable but inhibitory structures (Tight), which raises the question of how the enzyme processes structurally diverse RNA. In reviewing recent literature, we propose a bivalent trapping and an unwinding mechanism for CRISPR-Cas6 to interact with the Relaxed and the Tight repeat RNA, respectively. Both mechanisms aim to create an identical RNA conformation at the cleavage site for accurate processing.
Identifier: FSU_pmch_28493337 (IID), 10.1002/bies.201700019 (DOI), PMC5699886 (PMCID), 28493337 (RID), 28493337 (EID)
Keywords: CRISPR, Cas6, RNA binding proteins, Endoribonuclease, Protein-RNA interactions, Stem-loop
Grant Number: R01 GM099604
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at
Subject(s): Archaea/enzymology
Archaeal Proteins/metabolism
Bacterial Proteins/metabolism
CRISPR-Cas Systems
Nucleic Acid Conformation
RNA-Binding Proteins/metabolism
Persistent Link to This Record:
Host Institution: FSU
Is Part Of: BioEssays : news and reviews in molecular, cellular and developmental biology.
Issue: iss. 6, vol. 39

Choose the citation style.
Sefcikova, J., Roth, M., Yu, G., & Li, H. (2017). Cas6 processes tight and relaxed repeat RNA via multiple mechanisms: A hypothesis. Bioessays : News And Reviews In Molecular, Cellular And Developmental Biology. Retrieved from