You are here

Examination of the Role of L-Glutamate and Inosine 5'-Monophosphate in Hedonic Taste-Guided Behavior by Mice Lacking the T1R1 + T1R3 Receptor.

Title: An Examination of the Role of L-Glutamate and Inosine 5'-Monophosphate in Hedonic Taste-Guided Behavior by Mice Lacking the T1R1 + T1R3 Receptor.
8 views
0 downloads
Name(s): Blonde, Ginger D, author
Spector, Alan C, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-06-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The heterodimeric T1R1 + T1R3 receptor is considered critical for normal signaling of L-glutamate and 5'-ribonucleotides in the oral cavity. However, some taste-guided responsiveness remains in mice lacking one subunit of the receptor, suggesting that other receptors are sufficient to support some behaviors. Here, mice lacking both receptor subunits (KO) and wild-type (WT, both n = 13) mice were tested in a battery of behavioral tests. Mice were trained and tested in gustometers with a concentration series of Maltrin-580, a maltodextrin, in a brief-access test (10-s trials) as a positive control. Similar tests followed with monosodium glutamate (MSG) with and without the ribonucleotide inosine 5'-monophosphate (IMP), but always in the presence of the epithelial sodium channel blocker amiloride (A). Brief-access tests were repeated following short-term (30-min) and long-term (48-h) exposures to MSG + A + IMP and were also conducted with sodium gluconate replacing MSG. Finally, progressive ratio tests were conducted with Maltrin-580 or MSG + A + IMP, to assess appetitive behavior while minimizing satiation. Overall, MSG generated little concentration-dependent responding in either food-restricted WT or KO mice, even in combination with IMP. However, KO mice licked less to the amino acid stimuli, a measure of consummatory behavior in the brief-access tests. In contrast, both groups initiated a similar number of trials and had a similar breakpoint in the progressive ratio task, both measures of appetitive (approach) behavior. Collectively, these results suggest that while the T1R1 + T1R3 receptor is necessary for consummatory responding to MSG (+IMP), other receptors are sufficient to maintain appetitive responding to this "umami" stimulus complex in food-restricted mice.
Identifier: FSU_pmch_28334294 (IID), 10.1093/chemse/bjx015 (DOI), PMC6075476 (PMCID), 28334294 (RID), 28334294 (EID), 3073965 (PII)
Keywords: MSG, Amino acid, Knockout mice, Taste hedonics, Umami
Grant Number: R01 DC004574
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6075476.
Subject(s): Animals
Female
Glutamic Acid/administration & dosage
Glutamic Acid/pharmacology
Inosine Monophosphate/administration & dosage
Inosine Monophosphate/analogs & derivatives
Inosine Monophosphate/pharmacology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Philosophy
Receptors, G-Protein-Coupled/deficiency
Taste/drug effects
Taste/physiology
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28334294
Host Institution: FSU
Is Part Of: Chemical senses.
1464-3553
Issue: iss. 5, vol. 42

Choose the citation style.
Blonde, G. D., & Spector, A. C. (2017). An Examination of the Role of L-Glutamate and Inosine 5'-Monophosphate in Hedonic Taste-Guided Behavior by Mice Lacking the T1R1 + T1R3 Receptor. Chemical Senses. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28334294