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Enhanced cellular uptake of size-separated lipophilic silicon nanoparticles.

Title: Enhanced cellular uptake of size-separated lipophilic silicon nanoparticles.
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Name(s): Kusi-Appiah, Aubrey E, author
Mastronardi, Melanie L, author
Qian, Chenxi, author
Chen, Kenneth K, author
Ghazanfari, Lida, author
Prommapan, Plengchart, author
Kübel, Christian, author
Ozin, Geoffrey A, author
Lenhert, Steven, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-03-08
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Specific size, shape and surface chemistry influence the biological activity of nanoparticles. In the case of lipophilic nanoparticles, which are widely used in consumer products, there is evidence that particle size and formulation influences skin permeability and that lipophilic particles smaller than 6 nm can embed in lipid bilayers. Since most nanoparticle synthetic procedures result in mixtures of different particles, post-synthetic purification promises to provide insights into nanostructure-function relationships. Here we used size-selective precipitation to separate lipophilic allyl-benzyl-capped silicon nanoparticles into monodisperse fractions within the range of 1 nm to 5 nm. We measured liposomal encapsulation and cellular uptake of the monodisperse particles and found them to have generally low cytotoxicities in Hela cells. However, specific fractions showed reproducibly higher cytotoxicity than other fractions as well as the unseparated ensemble. Measurements indicate that the cytotoxicity mechanism involves oxidative stress and the differential cytotoxicity is due to enhanced cellular uptake by specific fractions. The results indicate that specific particles, with enhanced suitability for incorporation into lipophilic regions of liposomes and subsequent in vitro delivery to cells, are enriched in certain fractions.
Identifier: FSU_pmch_28272505 (IID), 10.1038/srep43731 (DOI), PMC5341124 (PMCID), 28272505 (RID), 28272505 (EID), srep43731 (PII)
Grant Number: R01 GM107172
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341124.
Subject(s): Biological Transport
Cell Survival
Fluorescent Antibody Technique
HeLa Cells
Humans
Hydrophobic and Hydrophilic Interactions
Liposomes
Nanoparticles/chemistry
Nanoparticles/ultrastructure
Particle Size
Silicon/chemistry
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28272505
Host Institution: FSU
Is Part Of: Scientific reports.
2045-2322
Issue: vol. 7

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Kusi-Appiah, A. E., Mastronardi, M. L., Qian, C., Chen, K. K., Ghazanfari, L., Prommapan, P., … Lenhert, S. (2017). Enhanced cellular uptake of size-separated lipophilic silicon nanoparticles. Scientific Reports. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28272505