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Differential Regulation of Cyclin E by Yorkie-Scalloped Signaling in Organ Development.

Title: Differential Regulation of Cyclin E by Yorkie-Scalloped Signaling in Organ Development.
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Name(s): Shu, Zhiqiang, author
Deng, Wu-Min, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-03-10
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Tissue integrity and homeostasis are accomplished through strict spatial and temporal regulation of cell growth and proliferation during development. Various signaling pathways have emerged as major growth regulators across metazoans; yet, how differential growth within a tissue is spatiotemporally coordinated remains largely unclear. Here, we report a role of a growth modulator Yorkie (), the homolog of Yes-associated protein (YAP), that differentially regulates its targets in wing imaginal discs; whereby Yki interacts with its transcriptional partner, Scalloped (), the homolog of the TEAD/TEF family transcription factor in mammals, to control an essential cell cycle regulator Cyclin E (CycE). Interestingly, when Yki was coexpressed with Fizzy-related (), a endocycle inducer and homolog of Cdh1 in mammals, surrounding hinge cells displayed larger nuclear size than distal pouch cells. The observed size difference is attributable to differential regulation of CycE, a target of Yki and Sd, the latter of which can directly bind to regulatory sequences, and is expressed only in the pouch region of the wing disc starting from the late second-instar larval stage. During earlier stages of larval development, when Sd expression was not detected in the wing disc, coexpression of Fzr and Yki did not cause size differences between cells along the proximal-distal axis of the disc. We show that ectopic CycE promoted cell proliferation and apoptosis, and inhibited transcriptional activity of Yki targets. These findings suggest that spatiotemporal expression of transcription factor Sd induces differential growth regulation by Yki during wing disc development, highlighting coordination between Yki and CycE to control growth and maintain homeostasis.
Identifier: FSU_pmch_28143945 (IID), 10.1534/g3.117.039065 (DOI), PMC5345706 (PMCID), 28143945 (RID), 28143945 (EID), g3.117.039065 (PII)
Keywords: Yki Sd, Cell growth and proliferation, Differential regulation
Grant Number: R01 GM072562
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345706.
Subject(s): Animals
Apoptosis/genetics
Cell Nucleus Size/genetics
Cell Proliferation/genetics
Cyclin E/metabolism
Drosophila Proteins/metabolism
Drosophila melanogaster/cytology
Drosophila melanogaster/embryology
Drosophila melanogaster/genetics
Drosophila melanogaster/metabolism
Endoreduplication/genetics
Gene Expression Regulation, Developmental
Homeostasis
Imaginal Discs/cytology
Imaginal Discs/metabolism
Models, Biological
Nuclear Proteins/metabolism
Organogenesis
Signal Transduction
Time Factors
Trans-Activators/metabolism
Transcription Factors/metabolism
Up-Regulation/genetics
Wings, Animal/cytology
Wings, Animal/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28143945
Owner Institution: FSU
Is Part Of: G3 (Bethesda, Md.).
2160-1836
Issue: iss. 3, vol. 7

Choose the citation style.
Shu, Z., & Deng, W. -M. (2017). Differential Regulation of Cyclin E by Yorkie-Scalloped Signaling in Organ Development. G3 (Bethesda, Md.). Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28143945