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Structural Heterogeneity in Pre-40S Ribosomes.

Title: Structural Heterogeneity in Pre-40S Ribosomes.
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Name(s): Johnson, Matthew C, author
Ghalei, Homa, author
Doxtader, Katelyn A, author
Karbstein, Katrin, author
Stroupe, M Elizabeth, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-02-07
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Late-stage 40S ribosome assembly is a highly regulated dynamic process that occurs in the cytoplasm, alongside the full translation machinery. Seven assembly factors (AFs) regulate and facilitate maturation, but the mechanisms through which they work remain undetermined. Here, we present a series of structures of the immature small subunit (pre-40S) determined by three-dimensional (3D) cryoelectron microscopy with 3D sorting to assess the molecule's heterogeneity. These structures demonstrate an extensive structural heterogeneity of interface AFs that likely regulates subunit joining during 40S maturation. We also present structural models for the beak and the platform, two regions where the low resolution of previous studies did not allow for localization of AFs and the rRNA, respectively. These models are supported by biochemical analyses using point variants and suggest that maturation of the 18S 3' end is regulated by dissociation of the AF Dim1 from the subunit interface, consistent with previous biochemical analyses.
Identifier: FSU_pmch_28111018 (IID), 10.1016/j.str.2016.12.011 (DOI), PMC5314460 (PMCID), 28111018 (RID), 28111018 (EID), S0969-2126(16)30401-4 (PII)
Keywords: Cryo-EM, Pre-40S ribosome, Ribosome assembly, Ribosome biogenesis, Small subunit assembly
Grant Number: R01 GM086451, R01 GM117093, S10 OD018142, S10 RR025080
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5314460.
Subject(s): Amino Acid Motifs
Binding Sites
Cloning, Molecular
Cryoelectron Microscopy
Crystallography, X-Ray
Escherichia coli/genetics
Escherichia coli/metabolism
Gene Expression
Genetic Heterogeneity
Humans
Models, Molecular
Nuclear Proteins/chemistry
Nuclear Proteins/genetics
Nuclear Proteins/metabolism
Protein Binding
Protein Interaction Domains and Motifs
Protein Structure, Secondary
RNA, Ribosomal/chemistry
RNA, Ribosomal/genetics
RNA, Ribosomal/metabolism
Recombinant Proteins/chemistry
Recombinant Proteins/genetics
Recombinant Proteins/metabolism
Ribonucleoprotein, U5 Small Nuclear/chemistry
Ribonucleoprotein, U5 Small Nuclear/genetics
Ribonucleoprotein, U5 Small Nuclear/metabolism
Ribosomal Proteins/chemistry
Ribosomal Proteins/genetics
Ribosomal Proteins/metabolism
Ribosome Subunits, Small, Eukaryotic/metabolism
Ribosome Subunits, Small, Eukaryotic/ultrastructure
Saccharomyces cerevisiae/metabolism
Saccharomyces cerevisiae/ultrastructure
Saccharomyces cerevisiae Proteins/chemistry
Saccharomyces cerevisiae Proteins/genetics
Saccharomyces cerevisiae Proteins/metabolism
Thermodynamics
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28111018
Owner Institution: FSU
Is Part Of: Structure (London, England : 1993).
1878-4186
Issue: iss. 2, vol. 25

Choose the citation style.
Johnson, M. C., Ghalei, H., Doxtader, K. A., Karbstein, K., & Stroupe, M. E. (2017). Structural Heterogeneity in Pre-40S Ribosomes. Structure (London, England : 1993). Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28111018