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Differential role of calpain-dependent protein cleavage in intermediate and long-term operant memory in Aplysia.

Title: Differential role of calpain-dependent protein cleavage in intermediate and long-term operant memory in Aplysia.
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Name(s): Lyons, Lisa C, author
Gardner, Jacob S, author
Lentsch, Cassidy T, author
Gandour, Catherine E, author
Krishnan, Harini C, author
Noakes, Eric J, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-01-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: In addition to protein synthesis, protein degradation or protein cleavage may be necessary for intermediate (ITM) and long-term memory (LTM) to remove molecular constraints, facilitate persistent kinase activity and modulate synaptic plasticity. Calpains, a family of conserved calcium dependent cysteine proteases, modulate synaptic function through protein cleavage. We used the marine mollusk Aplysia californica to investigate the in vivo role of calpains during intermediate and long-term operant memory formation using the learning that food is inedible (LFI) paradigm. A single LFI training session, in which the animal associates a specific netted seaweed with the failure to swallow, generates short (30min), intermediate (4-6h) and long-term (24h) memory. Using the calpain inhibitors calpeptin and MDL-28170, we found that ITM requires calpain activity for induction and consolidation similar to the previously reported requirements for persistent protein kinase C activity in intermediate-term LFI memory. The induction of LTM also required calpain activity. In contrast to ITM, calpain activity was not necessary for the molecular consolidation of LTM. Surprisingly, six hours after LFI training we found that calpain activity was necessary for LTM, although this is a time at which neither persistent PKC activity nor protein synthesis is required for the maintenance of long-term LFI memory. These results demonstrate that calpains function in multiple roles in vivo during associative memory formation.
Identifier: FSU_pmch_27913293 (IID), 10.1016/j.nlm.2016.11.018 (DOI), PMC6179366 (PMCID), 27913293 (RID), 27913293 (EID), S1074-7427(16)30358-6 (PII)
Keywords: Aplysia, Associative memory, Calpain, Learning, Protein cleavage, Protein kinase M
Grant Number: R01 MH081012, R21 NS088835
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6179366.
Subject(s): Animals
Aplysia
Association Learning/drug effects
Association Learning/physiology
Calpain/antagonists & inhibitors
Conditioning, Operant/drug effects
Conditioning, Operant/physiology
Cysteine Proteinase Inhibitors/pharmacology
Dipeptides/pharmacology
Memory/drug effects
Memory/physiology
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_27913293
Host Institution: FSU
Is Part Of: Neurobiology of learning and memory.
1095-9564
Issue: vol. 137

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Lyons, L. C., Gardner, J. S., Lentsch, C. T., Gandour, C. E., Krishnan, H. C., & Noakes, E. J. (2017). Differential role of calpain-dependent protein cleavage in intermediate and long-term operant memory in Aplysia. Neurobiology Of Learning And Memory. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_27913293