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Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis.

Title: A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis.
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Name(s): Fan, Jinbo, author
Jin, Hui, author
Yu, Hong-Guo, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-01-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: In this chapter, we describe a quantitative fluorescence-based assay of gene expression using the ratio of the reporter green fluorescence protein (GFP) to the internal red fluorescence protein (RFP) control. With this dual-color heterologous reporter assay, we have revealed cohesin-regulated genes and discovered a cis-acting DNA element, the Ty1-LTR, which interacts with cohesin and regulates gene expression during yeast meiosis. The method described here provides an effective cytological approach for quantitative analysis of global gene expression in budding yeast meiosis.
Identifier: FSU_pmch_27797078 (IID), 10.1007/978-1-4939-6545-8_9 (DOI), PMC5551346 (PMCID), 27797078 (RID), 27797078 (EID)
Keywords: Cohesin, Fluorescence-based reporter assay, Green fluorescence protein, Meiosis, Red fluorescence protein, Ty1-LTR
Grant Number: R01 GM117102
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5551346.
Subject(s): Cell Cycle Proteins/genetics
Chromosomal Proteins, Non-Histone/genetics
Chromosome Segregation/genetics
Gene Expression Regulation, Fungal
Genes, Reporter/genetics
Green Fluorescent Proteins/genetics
Luminescent Proteins/genetics
Meiosis/genetics
Molecular Biology/methods
Saccharomyces cerevisiae/genetics
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_27797078
Owner Institution: FSU
Is Part Of: Methods in molecular biology (Clifton, N.J.).
1940-6029
Issue: vol. 1515

Choose the citation style.
Fan, J., Jin, H., & Yu, H. -G. (2017). A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis. Methods In Molecular Biology (Clifton, N.j.). Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_27797078