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Expanding anchored hybrid enrichment to resolve both deep and shallow relationships within the spider tree of life.

Title: Expanding anchored hybrid enrichment to resolve both deep and shallow relationships within the spider tree of life.
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Name(s): Hamilton, Chris A, author
Lemmon, Alan R, author
Lemmon, Emily Moriarty, author
Bond, Jason E, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-10-13
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Despite considerable effort, progress in spider molecular systematics has lagged behind many other comparable arthropod groups, thereby hindering family-level resolution, classification, and testing of important macroevolutionary hypotheses. Recently, alternative targeted sequence capture techniques have provided molecular systematics a powerful tool for resolving relationships across the Tree of Life. One of these approaches, Anchored Hybrid Enrichment (AHE), is designed to recover hundreds of unique orthologous loci from across the genome, for resolving both shallow and deep-scale evolutionary relationships within non-model systems. Herein we present a modification of the AHE approach that expands its use for application in spiders, with a particular emphasis on the infraorder Mygalomorphae. Our aim was to design a set of probes that effectively capture loci informative at a diversity of phylogenetic timescales. Following identification of putative arthropod-wide loci, we utilized homologous transcriptome sequences from 17 species across all spiders to identify exon boundaries. Conserved regions with variable flanking regions were then sought across the tick genome, three published araneomorph spider genomes, and raw genomic reads of two mygalomorph taxa. Following development of the 585 target loci in the Spider Probe Kit, we applied AHE across three taxonomic depths to evaluate performance: deep-level spider family relationships (33 taxa, 327 loci); family and generic relationships within the mygalomorph family Euctenizidae (25 taxa, 403 loci); and species relationships in the North American tarantula genus Aphonopelma (83 taxa, 581 loci). At the deepest level, all three major spider lineages (the Mesothelae, Mygalomorphae, and Araneomorphae) were supported with high bootstrap support. Strong support was also found throughout the Euctenizidae, including generic relationships within the family and species relationships within the genus Aptostichus. As in the Euctenizidae, virtually identical topologies were inferred with high support throughout Aphonopelma. The Spider Probe Kit, the first implementation of AHE methodology in Class Arachnida, holds great promise for gathering the types and quantities of molecular data needed to accelerate an understanding of the spider Tree of Life by providing a mechanism whereby different researchers can confidently and effectively use the same loci for independent projects, yet allowing synthesis of data across independent research groups.
Identifier: FSU_pmch_27733110 (IID), 10.1186/s12862-016-0769-y (DOI), PMC5062932 (PMCID), 27733110 (RID), 27733110 (EID), 10.1186/s12862-016-0769-y (PII)
Keywords: Anchored Hybrid Enrichment, Anchored phylogenomics, Arachnida, Araneae, Conserved regions, Mygalomorphae, Targeted sequence capture, Ultraconserved elements
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5062932.
Subject(s): Animals
DNA Probes/metabolism
Genome
Hybridization, Genetic
Likelihood Functions
Phylogeny
Sequence Analysis, DNA
Species Specificity
Spiders/genetics
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_27733110
Owner Institution: FSU
Is Part Of: BMC evolutionary biology.
1471-2148
Issue: iss. 1, vol. 16

Choose the citation style.
Hamilton, C. A., Lemmon, A. R., Lemmon, E. M., & Bond, J. E. (2016). Expanding anchored hybrid enrichment to resolve both deep and shallow relationships within the spider tree of life. Bmc Evolutionary Biology. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_27733110