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DEAD-box Protein Rok1 Orchestrates 40S and 60S Ribosome Assembly by Promoting the Release of Rrp5 from Pre-40S Ribosomes to Allow for 60S Maturation.

Title: The DEAD-box Protein Rok1 Orchestrates 40S and 60S Ribosome Assembly by Promoting the Release of Rrp5 from Pre-40S Ribosomes to Allow for 60S Maturation.
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Name(s): Khoshnevis, Sohail, author
Askenasy, Isabel, author
Johnson, Matthew C, author
Dattolo, Maria D, author
Young-Erdos, Crystal L, author
Stroupe, M Elizabeth, author
Karbstein, Katrin, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-06-09
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: DEAD-box proteins are ubiquitous regulators of RNA biology. While commonly dubbed "helicases," their activities also include duplex annealing, adenosine triphosphate (ATP)-dependent RNA binding, and RNA-protein complex remodeling. Rok1, an essential DEAD-box protein, and its cofactor Rrp5 are required for ribosome assembly. Here, we use in vivo and in vitro biochemical analyses to demonstrate that ATP-bound Rok1, but not adenosine diphosphate (ADP)-bound Rok1, stabilizes Rrp5 binding to 40S ribosomes. Interconversion between these two forms by ATP hydrolysis is required for release of Rrp5 from pre-40S ribosomes in vivo, thereby allowing Rrp5 to carry out its role in 60S subunit assembly. Furthermore, our data also strongly suggest that the previously described accumulation of snR30 upon Rok1 inactivation arises because Rrp5 release is blocked and implicate a previously undescribed interaction between Rrp5 and the DEAD-box protein Has1 in mediating snR30 accumulation when Rrp5 release from pre-40S subunits is blocked.
Identifier: FSU_pmch_27280440 (IID), 10.1371/journal.pbio.1002480 (DOI), PMC4900678 (PMCID), 27280440 (RID), 27280440 (EID), PBIOLOGY-D-16-00944 (PII)
Grant Number: P41 GM103311, R01 GM086451
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900678.
Subject(s): Adenosine Diphosphate/metabolism
Adenosine Triphosphate/metabolism
Binding Sites/genetics
DEAD-box RNA Helicases/chemistry
DEAD-box RNA Helicases/genetics
DEAD-box RNA Helicases/metabolism
Hydrolysis
Models, Molecular
Molecular Conformation
Nuclear Proteins/chemistry
Nuclear Proteins/genetics
Nuclear Proteins/metabolism
Protein Binding
Protein Domains
RNA, Fungal/chemistry
RNA, Fungal/genetics
RNA, Fungal/metabolism
RNA, Small Nucleolar/chemistry
RNA, Small Nucleolar/genetics
RNA, Small Nucleolar/metabolism
RNA-Binding Proteins/chemistry
RNA-Binding Proteins/genetics
RNA-Binding Proteins/metabolism
Ribosome Subunits, Large, Eukaryotic/chemistry
Ribosome Subunits, Large, Eukaryotic/genetics
Ribosome Subunits, Large, Eukaryotic/metabolism
Ribosome Subunits, Small, Eukaryotic/chemistry
Ribosome Subunits, Small, Eukaryotic/genetics
Ribosome Subunits, Small, Eukaryotic/metabolism
Saccharomyces cerevisiae/genetics
Saccharomyces cerevisiae/metabolism
Saccharomyces cerevisiae Proteins/chemistry
Saccharomyces cerevisiae Proteins/genetics
Saccharomyces cerevisiae Proteins/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_27280440
Owner Institution: FSU
Is Part Of: PLoS biology.
1545-7885
Issue: iss. 6, vol. 14

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Khoshnevis, S., Askenasy, I., Johnson, M. C., Dattolo, M. D., Young-Erdos, C. L., Stroupe, M. E., & Karbstein, K. (2016). The DEAD-box Protein Rok1 Orchestrates 40S and 60S Ribosome Assembly by Promoting the Release of Rrp5 from Pre-40S Ribosomes to Allow for 60S Maturation. Plos Biology. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_27280440