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Quantitative assessment of fluorescent proteins.

Title: Quantitative assessment of fluorescent proteins.
Name(s): Cranfill, Paula J, author
Sell, Brittney R, author
Baird, Michelle A, author
Allen, John R, author
Lavagnino, Zeno, author
de Gruiter, H Martijn, author
Kremers, Gert-Jan, author
Davidson, Michael W, author
Ustione, Alessandro, author
Piston, David W, author
Type of Resource: text
Genre: Journal Article
Date Issued: 2016-07-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of bright and stable FPs spanning blue to red spectral regions. Common to autofluorescent FPs is their tight β-barrel structure, which provides the rigidity and chemical environment needed for effectual fluorescence. Despite the common structure, each FP has unique properties. Thus, there is no single 'best' FP for every circumstance, and each FP has advantages and disadvantages. To guide decisions about which FP is right for a given application, we have quantitatively characterized the brightness, photostability, pH stability and monomeric properties of more than 40 FPs to enable straightforward and direct comparison between them. We focus on popular and/or top-performing FPs in each spectral region.
Identifier: FSU_pmch_27240257 (IID), 10.1038/nmeth.3891 (DOI), PMC4927352 (PMCID), 27240257 (RID), 27240257 (EID), nmeth.3891 (PII)
Grant Number: S10 RR025649, R01 DK085064, R01 DK098659, S10 OD010681, P20 GM072048
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at
Subject(s): Fluorescence
HeLa Cells
Luminescent Proteins/analysis
Microscopy, Fluorescence/methods
Recombinant Fusion Proteins/analysis
Spectrometry, Fluorescence/methods
Persistent Link to This Record:
Owner Institution: FSU
Is Part Of: Nature methods.
Issue: iss. 7, vol. 13

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Cranfill, P. J., Sell, B. R., Baird, M. A., Allen, J. R., Lavagnino, Z., De Gruiter, H. M., … Piston, D. W. (2016). Quantitative assessment of fluorescent proteins. Nature Methods. Retrieved from