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Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype.

Title: Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype.
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Name(s): Rao, Anand N, author
Falnikar, Aditi, author
O'Toole, Eileen T, author
Morphew, Mary K, author
Hoenger, Andreas, author
Davidson, Michael W, author
Yuan, Xiaobing, author
Baas, Peter W, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-05-09
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Contemporary models for neuronal migration are grounded in the view that virtually all functionally relevant microtubules (MTs) in migrating neurons are attached to the centrosome, which occupies a position between the nucleus and a short leading process. It is assumed that MTs do not undergo independent movements but rather transduce forces that enable movements of the centrosome and nucleus. The present results demonstrate that although this is mostly true, a small fraction of the MTs are centrosome-unattached, and this permits limited sliding of MTs. When this sliding is pharmacologically inhibited, the leading process becomes shorter, migration of the neuron deviates from its normal path, and the MTs within the leading process become buckled. Partial depletion of ninein, a protein that attaches MTs to the centrosome, leads to greater numbers of centrosome-unattached MTs as well as greater sliding of MTs. Concomitantly, the soma becomes less mobile and the leading process acquires an elongated morphology akin to an axon.
Identifier: FSU_pmch_27138250 (IID), 10.1083/jcb.201506140 (DOI), PMC4862329 (PMCID), 27138250 (RID), 27138250 (EID), jcb.201506140 (PII)
Grant Number: F31 NS093748, P41 GM103431, R01 NS028785, R56 NS028785
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4862329.
Subject(s): Animals
Cell Movement/physiology
Centrosome/metabolism
Centrosome/ultrastructure
Cytoskeletal Proteins/genetics
Cytoskeletal Proteins/metabolism
Cytoskeletal Proteins/physiology
Microscopy, Electron, Transmission
Microtubule-Associated Proteins/genetics
Microtubule-Associated Proteins/metabolism
Microtubule-Associated Proteins/physiology
Microtubules/metabolism
Microtubules/ultrastructure
Neurons/metabolism
Neurons/physiology
Neurons/ultrastructure
Nuclear Proteins/genetics
Nuclear Proteins/metabolism
Nuclear Proteins/physiology
Phenotype
RNA Interference
Rats
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_27138250
Owner Institution: FSU
Is Part Of: The Journal of cell biology.
1540-8140
Issue: iss. 3, vol. 213

Choose the citation style.
Rao, A. N., Falnikar, A., O'Toole, E. T., Morphew, M. K., Hoenger, A., Davidson, M. W., … Baas, P. W. (2016). Sliding of centrosome-unattached microtubules defines key features of neuronal phenotype. The Journal Of Cell Biology. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_27138250