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Ecdysone and Notch Pathways Synergistically Regulate Cut at the Dorsal-Ventral Boundary in Drosophila Wing Discs.

Title: The Ecdysone and Notch Pathways Synergistically Regulate Cut at the Dorsal-Ventral Boundary in Drosophila Wing Discs.
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Name(s): Jia, Dongyu, author
Bryant, Jamal, author
Jevitt, Allison, author
Calvin, Gabriel, author
Deng, Wu-Min, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-04-20
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Metazoan development requires coordination of signaling pathways to regulate patterns of gene expression. In Drosophila, the wing imaginal disc provides an excellent model for the study of how signaling pathways interact to regulate pattern formation. The determination of the dorsal-ventral (DV) boundary of the wing disc depends on the Notch pathway, which is activated along the DV boundary and induces the expression of the homeobox transcription factor, Cut. Here, we show that Broad (Br), a zinc-finger transcription factor, is also involved in regulating Cut expression in the DV boundary region. However, Br expression is not regulated by Notch signaling in wing discs, while ecdysone signaling is the upstream signal that induces Br for Cut upregulation. Also, we find that the ecdysone-Br cascade upregulates cut-lacZ expression, a reporter containing a 2.7 kb cut enhancer region, implying that ecdysone signaling, similar to Notch, regulates cut at the transcriptional level. Collectively, our findings reveal that the Notch and ecdysone signaling pathways synergistically regulate Cut expression for proper DV boundary formation in the wing disc. Additionally, we show br promotes Delta, a Notch ligand, near the DV boundary to suppress aberrant high Notch activity, indicating further interaction between the two pathways for DV patterning of the wing disc.
Identifier: FSU_pmch_27117286 (IID), 10.1016/j.jgg.2016.03.002 (DOI), PMC5391978 (PMCID), 27117286 (RID), 27117286 (EID), S1673-8527(16)30017-0 (PII)
Keywords: Broad, Cut, Dorsal–ventral boundary, Drosophila, Ecdysone, Notch, Wing disc
Grant Number: R01 GM072562, R01 GM084947
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391978.
Subject(s): Animals
Drosophila Proteins/genetics
Drosophila Proteins/metabolism
Drosophila melanogaster/anatomy & histology
Drosophila melanogaster/cytology
Drosophila melanogaster/genetics
Drosophila melanogaster/growth & development
Ecdysone/metabolism
Homeodomain Proteins/genetics
Homeodomain Proteins/metabolism
Intracellular Signaling Peptides and Proteins/metabolism
Membrane Proteins/metabolism
Nuclear Proteins/genetics
Nuclear Proteins/metabolism
Receptors, Notch/metabolism
Signal Transduction
Transcription Factors/genetics
Transcription Factors/metabolism
Transcription, Genetic
Up-Regulation
Wings, Animal/anatomy & histology
Wings, Animal/growth & development
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_27117286
Owner Institution: FSU
Is Part Of: Journal of genetics and genomics = Yi chuan xue bao.
1673-8527
Issue: iss. 4, vol. 43

Choose the citation style.
Jia, D., Bryant, J., Jevitt, A., Calvin, G., & Deng, W. -M. (2016). The Ecdysone and Notch Pathways Synergistically Regulate Cut at the Dorsal-Ventral Boundary in Drosophila Wing Discs. Journal Of Genetics And Genomics = Yi Chuan Xue Bao. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_27117286