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Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6.

Title: A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6.
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Name(s): Shao, Yaming, author
Richter, Hagen, author
Sun, Shengfang, author
Sharma, Kundan, author
Urlaub, Henning, author
Randau, Lennart, author
Li, Hong, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-04-05
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: A subclass of recently discovered CRISPR repeat RNA in bacteria contains minimally recognizable structural features that facilitate an unknown mechanism of recognition and processing by the Cas6 family of endoribonucleases. Cocrystal structures of Cas6 from Methanococcus maripaludis (MmCas6b) bound with its repeat RNA revealed a dual site binding structure and a cleavage site conformation poised for phosphodiester bond breakage. Two non-interacting MmCas6b bind to two separate AAYAA motifs within the same repeat, one distal and one adjacent to the cleavage site. This bound structure potentially competes with a stable but non-productive RNA structure. At the cleavage site, MmCas6b supplies a base pair mimic to stabilize a short 2 base pair stem immediately upstream of the scissile phosphate. Complementary biochemical analyses support the dual-AAYAA binding model and a critical role of the protein-RNA base pair mimic. Our results reveal a previously unknown method of processing non-stem-loop CRISPR RNA by Cas6.
Identifier: FSU_pmch_26996962 (IID), 10.1016/j.str.2016.02.009 (DOI), PMC4823167 (PMCID), 26996962 (RID), 26996962 (EID), S0969-2126(16)00068-X (PII)
Keywords: CRISPR RNA, Crystal structures, Endoribonucleases, Protein-RNA interactions
Grant Number: R01 GM099604, NIH-NIGMS SC3GM113805
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823167.
Subject(s): Archaeal Proteins/chemistry
Archaeal Proteins/metabolism
Binding Sites
Catalytic Domain
Clustered Regularly Interspaced Short Palindromic Repeats
Crystallography, X-Ray
Endoribonucleases/chemistry
Endoribonucleases/metabolism
Methanococcus/chemistry
Methanococcus/genetics
Models, Molecular
Nucleic Acid Conformation
Protein Binding
Protein Conformation
RNA, Archaeal/chemistry
RNA, Archaeal/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_26996962
Owner Institution: FSU
Is Part Of: Structure (London, England : 1993).
1878-4186
Issue: iss. 4, vol. 24

Choose the citation style.
Shao, Y., Richter, H., Sun, S., Sharma, K., Urlaub, H., Randau, L., & Li, H. (2016). A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6. Structure (London, England : 1993). Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_26996962