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improved smaller biotin ligase for BioID proximity labeling.

Title: An improved smaller biotin ligase for BioID proximity labeling.
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Name(s): Kim, Dae In, author
Jensen, Samuel C, author
Noble, Kyle A, author
Kc, Birendra, author
Roux, Kenneth H, author
Motamedchaboki, Khatereh, author
Roux, Kyle J, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-04-15
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The BioID method uses a promiscuous biotin ligase to detect protein-protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate proteins. Thus BioID2 improves the efficiency of screening for protein-protein associations. We also demonstrate that the biotinylation range of BioID2 can be considerably modulated using flexible linkers, thus enabling application-specific adjustment of the biotin-labeling radius.
Identifier: FSU_pmch_26912792 (IID), 10.1091/mbc.E15-12-0844 (DOI), PMC4831873 (PMCID), 26912792 (RID), 26912792 (EID), mbc.E15-12-0844 (PII)
Grant Number: P20GM103620, R01EB014869, R01 GM102486, R01 EB014869, P20 GM103620, R01 GM102203, P30 CA030199, R01GM102486, R01GM102203, P20 GM103548
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4831873.
Subject(s): Animals
Biotin/metabolism
Biotinylation
Carbon-Nitrogen Ligases/genetics
Carbon-Nitrogen Ligases/metabolism
Escherichia coli Proteins/genetics
Escherichia coli Proteins/metabolism
Humans
Intracellular Signaling Peptides and Proteins/genetics
Intracellular Signaling Peptides and Proteins/metabolism
Membrane Proteins/genetics
Membrane Proteins/metabolism
Mice
Molecular Biology/methods
NIH 3T3 Cells
Nuclear Pore Complex Proteins/genetics
Nuclear Pore Complex Proteins/metabolism
Nuclear Proteins/genetics
Nuclear Proteins/metabolism
Protein Engineering/methods
Protein Interaction Mapping/methods
Recombinant Fusion Proteins/genetics
Recombinant Fusion Proteins/metabolism
Repressor Proteins/genetics
Repressor Proteins/metabolism
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_26912792
Owner Institution: FSU
Is Part Of: Molecular biology of the cell.
1939-4586
Issue: iss. 8, vol. 27

Choose the citation style.
Kim, D. I., Jensen, S. C., Noble, K. A., Kc, B., Roux, K. H., Motamedchaboki, K., & Roux, K. J. (2016). An improved smaller biotin ligase for BioID proximity labeling. Molecular Biology Of The Cell. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_26912792