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Novel Role of Blackberries in Senescence and Atherosclerosis in ApoE-/- Mice

Title: Novel Role of Blackberries in Senescence and Atherosclerosis in ApoE-/- Mice.
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Name(s): Serino, Alexa, author
Type of Resource: text
Genre: Text
Bachelor Thesis
Date Issued: 2018-11-30
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Cardiovascular disease is the leading cause of death worldwide, and the incidence of these diseases increases with age. Senescence is an aging process that results in the termination of cell proliferation due to multiple cell divisions that shorten telomeres and damage DNA. Senescence can also be induced prematurely by cellular stress including reactive oxygen species (ROS), produced by several sources including nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (Nox) enzymes. Excessive production of cellular ROS is maintained by antioxidant enzymes including superoxide dismutase (SOD) 1 and 2 and catalase. Published data from our lab showed that blackberry polyphenols increase SOD1 expression and decrease expression of Nox1, decreasing ROS levels and reducing senescence in vitro in vascular smooth muscle cells (VSMCs). Through the use of ApoE-/- male mice, preliminary data show that supplementation with 2% freeze-dried blackberry powder reduced senescence and plaque in response to high fat diet in the aorta. However, it is unknown whether the protective effect of blackberry is mediated by downregulation of Nox1 in vivo and whether this effect is also observed in female mice. In this dissertation, we will test the hypothesis that the protective effect of blackberry supplementation in reducing senescence and atherosclerosis is associated with the downregulation of Nox1 in vivo in both male and female ApoE-/- mice. After 5 weeks of treatment with low fat (LFD), high fat (HFD), or HFD supplemented with blackberry (HFD+BL), results showed that blackberry reduces Nox1 expression in male aortas. Blackberry further protects against ROS production by reducing activation of Angiotensin II (Ang II) targets including p38 mitogen activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK), and peroxisome proliferator-activated receptor coactivator-1 (PGC-1a). In female aortas, blackberry upregulates antioxidant enzyme SOD2.
Identifier: FSU_libsubv1_scholarship_submission_1543622432_b8525004 (IID)
Keywords: senescence, atherosclerosis, blueberry, polyphenol, Nox
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1543622432_b8525004
Owner Institution: FSU

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Serino, A. (2018). Novel Role of Blackberries in Senescence and Atherosclerosis in ApoE-/- Mice. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1543622432_b8525004