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Alpha- and Gammaproteobacterial Methanotrophs Codominate the Active Methane-Oxidizing Communities in an Acidic Boreal Peat Bog.

Title: Alpha- and Gammaproteobacterial Methanotrophs Codominate the Active Methane-Oxidizing Communities in an Acidic Boreal Peat Bog.
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Name(s): Esson, Kaitlin C, author
Lin, Xueju, author
Kumaresan, Deepak, author
Chanton, Jeffrey P, author
Murrell, J Colin, author
Kostka, Joel E, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-04-04
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The objective of this study was to characterize metabolically active, aerobic methanotrophs in an ombrotrophic peatland in the Marcell Experimental Forest, in Minnesota. Methanotrophs were investigated in the field and in laboratory incubations using DNA-stable isotope probing (SIP), expression studies on particulate methane monooxygenase (pmoA) genes, and amplicon sequencing of 16S rRNA genes. Potential rates of oxidation ranged from 14 to 17 μmol of CH4g dry weight soil(-1)day(-1) Within DNA-SIP incubations, the relative abundance of methanotrophs increased from 4% in situ to 25 to 36% after 8 to 14 days. Phylogenetic analysis of the(13)C-enriched DNA fractions revealed that the active methanotrophs were dominated by the genera Methylocystis(type II;Alphaproteobacteria),Methylomonas, and Methylovulum(both, type I;Gammaproteobacteria). In field samples, a transcript-to-gene ratio of 1 to 2 was observed for pmoA in surface peat layers, which attenuated rapidly with depth, indicating that the highest methane consumption was associated with a depth of 0 to 10 cm. Metagenomes and sequencing of cDNA pmoA amplicons from field samples confirmed that the dominant active methanotrophs were Methylocystis and Methylomonas Although type II methanotrophs have long been shown to mediate methane consumption in peatlands, our results indicate that members of the genera Methylomonas and Methylovulum(type I) can significantly contribute to aerobic methane oxidation in these ecosystems.
Identifier: FSU_pmch_26873322 (IID), 10.1128/AEM.03640-15 (DOI), PMC4959502 (PMCID), 26873322 (RID), 26873322 (EID), AEM.03640-15 (PII)
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959502.
Subject(s): Aerobiosis
Alphaproteobacteria/classification
Alphaproteobacteria/genetics
Alphaproteobacteria/isolation & purification
Alphaproteobacteria/metabolism
Biota
Cluster Analysis
DNA, Bacterial/chemistry
DNA, Bacterial/genetics
DNA, Ribosomal/chemistry
DNA, Ribosomal/genetics
Environmental Microbiology
Gammaproteobacteria/classification
Gammaproteobacteria/genetics
Gammaproteobacteria/isolation & purification
Gammaproteobacteria/metabolism
Metagenome
Methane/metabolism
Minnesota
Oxidation-Reduction
Oxygenases/genetics
Phylogeny
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Wetlands
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_26873322
Owner Institution: FSU
Is Part Of: Applied and environmental microbiology.
1098-5336
Issue: iss. 8, vol. 82

Choose the citation style.
Esson, K. C., Lin, X., Kumaresan, D., Chanton, J. P., Murrell, J. C., & Kostka, J. E. (2016). Alpha- and Gammaproteobacterial Methanotrophs Codominate the Active Methane-Oxidizing Communities in an Acidic Boreal Peat Bog. Applied And Environmental Microbiology. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_26873322