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Directional memory arises from long-lived cytoskeletal asymmetries in polarized chemotactic cells.

Title: Directional memory arises from long-lived cytoskeletal asymmetries in polarized chemotactic cells.
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Name(s): Prentice-Mott, Harrison V, author
Meroz, Yasmine, author
Carlson, Andreas, author
Levine, Michael A, author
Davidson, Michael W, author
Irimia, Daniel, author
Charras, Guillaume T, author
Mahadevan, L, author
Shah, Jagesh V, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-02-02
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Chemotaxis, the directional migration of cells in a chemical gradient, is robust to fluctuations associated with low chemical concentrations and dynamically changing gradients as well as high saturating chemical concentrations. Although a number of reports have identified cellular behavior consistent with a directional memory that could account for behavior in these complex environments, the quantitative and molecular details of such a memory process remain unknown. Using microfluidics to confine cellular motion to a 1D channel and control chemoattractant exposure, we observed directional memory in chemotactic neutrophil-like cells. We modeled this directional memory as a long-lived intracellular asymmetry that decays slower than observed membrane phospholipid signaling. Measurements of intracellular dynamics revealed that moesin at the cell rear is a long-lived element that when inhibited, results in a reduction of memory. Inhibition of ROCK (Rho-associated protein kinase), downstream of RhoA (Ras homolog gene family, member A), stabilized moesin and directional memory while depolymerization of microtubules (MTs) disoriented moesin deposition and also reduced directional memory. Our study reveals that long-lived polarized cytoskeletal structures, specifically moesin, actomyosin, and MTs, provide a directional memory in neutrophil-like cells even as they respond on short time scales to external chemical cues.
Identifier: FSU_pmch_26764383 (IID), 10.1073/pnas.1513289113 (DOI), PMC4747767 (PMCID), 26764383 (RID), 26764383 (EID), 1513289113 (PII)
Keywords: Cell polarization, Chemotaxis, Confined cell migration, Microtubules, Moesin
Grant Number: BB/F021402, BB/F021402/1, T32 GM008313, R01 GM092804, P41 EB002503
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747767.
Subject(s): Cell Polarity
Chemotaxis
Cytoskeleton/metabolism
HL-60 Cells
Humans
Immunologic Memory
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_26764383
Owner Institution: FSU
Is Part Of: Proceedings of the National Academy of Sciences of the United States of America.
1091-6490
Issue: iss. 5, vol. 113

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Prentice-Mott, H. V., Meroz, Y., Carlson, A., Levine, M. A., Davidson, M. W., Irimia, D., … Shah, J. V. (2016). Directional memory arises from long-lived cytoskeletal asymmetries in polarized chemotactic cells. Proceedings Of The National Academy Of Sciences Of The United States Of America. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_26764383