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Automatic stage identification of Drosophila egg chamber based on DAPI images.

Title: Automatic stage identification of Drosophila egg chamber based on DAPI images.
Name(s): Jia, Dongyu, author
Xu, Qiuping, author
Xie, Qian, author
Mio, Washington, author
Deng, Wu-Min, author
Type of Resource: text
Genre: Journal Article
Date Issued: 2016-01-06
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The Drosophila egg chamber, whose development is divided into 14 stages, is a well-established model for developmental biology. However, visual stage determination can be a tedious, subjective and time-consuming task prone to errors. Our study presents an objective, reliable and repeatable automated method for quantifying cell features and classifying egg chamber stages based on DAPI images. The proposed approach is composed of two steps: 1) a feature extraction step and 2) a statistical modeling step. The egg chamber features used are egg chamber size, oocyte size, egg chamber ratio and distribution of follicle cells. Methods for determining the on-site of the polytene stage and centripetal migration are also discussed. The statistical model uses linear and ordinal regression to explore the stage-feature relationships and classify egg chamber stages. Combined with machine learning, our method has great potential to enable discovery of hidden developmental mechanisms.
Identifier: FSU_pmch_26732176 (IID), 10.1038/srep18850 (DOI), PMC4702167 (PMCID), 26732176 (RID), 26732176 (EID), srep18850 (PII)
Grant Number: R01 GM072562, R01GM072562
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at
Subject(s): Animals
Cell Movement
Drosophila/anatomy & histology
Germ Cells/cytology
Germ Cells/metabolism
Germ-Line Mutation
Microscopy, Fluorescence
Ovarian Follicle/cytology
Ovarian Follicle/metabolism
Ovary/anatomy & histology
Persistent Link to This Record:
Host Institution: FSU
Is Part Of: Scientific reports.
Issue: vol. 6

Choose the citation style.
Jia, D., Xu, Q., Xie, Q., Mio, W., & Deng, W. -M. (2016). Automatic stage identification of Drosophila egg chamber based on DAPI images. Scientific Reports. Retrieved from