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Interfacing Microfluidics with Negative Stain Transmission Electron Microscopy.

Title: Interfacing Microfluidics with Negative Stain Transmission Electron Microscopy.
Name(s): Mukhitov, Nikita, author
Spear, John M, author
Stagg, Scott M, author
Roper, Michael G, author
Type of Resource: text
Genre: Journal Article
Date Issued: 2016-01-05
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: A microfluidic platform is presented for preparing negatively stained grids for use in transmission electron microscopy (EM). The microfluidic device is composed of glass etched with readily fabricated features that facilitate the extraction of the grid poststaining and maintains the integrity of the sample. Utilization of this device simultaneously reduced environmental contamination on the grids and improved the homogeneity of the heavy metal stain needed to enhance visualization of biological specimens as compared to conventionally prepared EM grids. This easy-to-use EM grid preparation device provides the basis for future developments of systems with more integrated features, which will allow for high-throughput and dynamic structural biology studies.
Identifier: FSU_pmch_26642355 (IID), 10.1021/acs.analchem.5b03884 (DOI), PMC4730115 (PMCID), 26642355 (RID), 26642355 (EID)
Grant Number: R01 GM108753, S10 OD018142
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at
Subject(s): Animals
Cell Line
Metals, Heavy/chemistry
Microfluidic Analytical Techniques/instrumentation
Microscopy, Electron, Transmission/instrumentation
Microscopy, Electron, Transmission/methods
Models, Molecular
Particle Size
Potassium Channels, Voltage-Gated/isolation & purification
Potassium Channels, Voltage-Gated/ultrastructure
Staining and Labeling/methods
Persistent Link to This Record:
Owner Institution: FSU
Is Part Of: Analytical chemistry.
Issue: iss. 1, vol. 88

Choose the citation style.
Mukhitov, N., Spear, J. M., Stagg, S. M., & Roper, M. G. (2016). Interfacing Microfluidics with Negative Stain Transmission Electron Microscopy. Analytical Chemistry. Retrieved from