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Expression Differentiation Is Constrained to Low-Expression Proteins over Ecological Timescales.

Title: Expression Differentiation Is Constrained to Low-Expression Proteins over Ecological Timescales.
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Name(s): Margres, Mark J, author
Wray, Kenneth P, author
Seavy, Margaret, author
McGivern, James J, author
Herrera, Nathanael D, author
Rokyta, Darin R, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2016-01-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Protein expression level is one of the strongest predictors of protein sequence evolutionary rate, with high-expression protein sequences evolving at slower rates than low-expression protein sequences largely because of constraints on protein folding and function. Expression evolutionary rates also have been shown to be negatively correlated with expression level across human and mouse orthologs over relatively long divergence times (i.e., ∼100 million years). Long-term evolutionary patterns, however, often cannot be extrapolated to microevolutionary processes (and vice versa), and whether this relationship holds for traits evolving under directional selection within a single species over ecological timescales (i.e., <5000 years) is unknown and not necessarily expected. Expression is a metabolically costly process, and the expression level of a particular protein is predicted to be a tradeoff between the benefit of its function and the costs of its expression. Selection should drive the expression level of all proteins close to values that maximize fitness, particularly for high-expression proteins because of the increased energetic cost of production. Therefore, stabilizing selection may reduce the amount of standing expression variation for high-expression proteins, and in combination with physiological constraints that may place an upper bound on the range of beneficial expression variation, these constraints could severely limit the availability of beneficial expression variants. To determine whether rapid-expression evolution was restricted to low-expression proteins owing to these constraints on highly expressed proteins over ecological timescales, we compared venom protein expression levels across mainland and island populations for three species of pit vipers. We detected significant differentiation in protein expression levels in two of the three species and found that rapid-expression differentiation was restricted to low-expression proteins. Our results suggest that various constraints on high-expression proteins reduce the availability of beneficial expression variants relative to low-expression proteins, enabling low-expression proteins to evolve and potentially lead to more rapid adaptation.
Identifier: FSU_pmch_26546003 (IID), 10.1534/genetics.115.180547 (DOI), PMC4701091 (PMCID), 26546003 (RID), 26546003 (EID), genetics.115.180547 (PII)
Keywords: Adaptation, Evolutionary rates, Protein expression, Selective constraints
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4701091.
Subject(s): Animals
Ecosystem
Evolution, Molecular
Gene Expression
Gene Expression Profiling
Islands
Proteins/genetics
Sequence Analysis, DNA
Southeastern United States
Time
Viperidae/genetics
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_26546003
Owner Institution: FSU
Is Part Of: Genetics.
1943-2631
Issue: iss. 1, vol. 202

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Margres, M. J., Wray, K. P., Seavy, M., McGivern, J. J., Herrera, N. D., & Rokyta, D. R. (2016). Expression Differentiation Is Constrained to Low-Expression Proteins over Ecological Timescales. Genetics. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_26546003