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Microrna-22 Inhibits The Proliferation And Migration, And Increases The Cisplatin Sensitivity, Of Osteosarcoma Cells

Title: Microrna-22 Inhibits The Proliferation And Migration, And Increases The Cisplatin Sensitivity, Of Osteosarcoma Cells.
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Name(s): Zhou, Xiang, author
Natino, Dimple, author
Zhai, Xu, author
Gao, Zhongyang, author
He, Xijing, author
Type of Resource: text
Genre: Journal Article
Text
Journal Article
Date Issued: 2018-05-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Osteosarcoma (OS) is the major type of primary bone tumor and is associated with a poor prognosis due to chemotherapy resistance. Accumulating evidence indicates that microRNAs (miRNAs/miRs) may influence the tumor progression of OS and cell sensitivity to chemotherapy. In the present study, a total of 7 patients with OS and 7 healthy volunteers were recruited. Reverse transcription-quantitative polymerase chain reaction and ELISA were performed to determine the expression of miRNAs and mRNAs in the serum of participants. Furthermore, the biological function of miR-22 and S100A11 was examined in MG-63 cells using Cell Counting Kit-8 assays, Transwell migration assays and western blot analysis to determine the effects on cell proliferation, migration and protein expression, respectively, while MG-63 cell sensitivity to cisplatin was assessed by measuring cell viability following cisplatin treatment and calculating the half maximal inhibitory concentration (IC50). Additionally, the association between miR-22 and S100 calcium-binding protein A11 (S100A11) was validated using a luciferase reporter assay. The results demonstrated that miR-22 expression was significantly reduced in patients with OS and the MG-63 OS cell line, compared with healthy volunteers and the normal osteoblast hFOB 1.19 cell line, respectively, while the expression of S100A11 was negatively associated with miR-22 levels in the MG-63 cell line. Furthermore, overexpression of miR-22 inhibited the proliferation and migratory ability of MG-63 cells, and increased the sensitivity of MG-63 cells to cisplatin treatment; however, overexpression of S100A11 partially attenuated the alterations in proliferation, migratory ability and chemosensitivity that were induced by miR-22 overexpression. In addition, it was confirmed that S100A11 is a direct target gene of miR-22 in MG-63 cells. In conclusion, to the best of our knowledge, the present study is the first to demonstrate that miR-22 may be a promising therapeutic target and may have potential as part of a combination treatment alongside chemotherapeutic agents for OS.
Identifier: FSU_libsubv1_wos_000430556800125 (IID), 10.3892/mmr.2018.8790 (DOI)
Keywords: growth, expression, mediator, proliferation, cancer-cells, keratinocytes, colorectal-cancer, migration, cisplatin, chemotherapy, microRNA, osteosarcoma, rnas, s100a11, sp1
Publication Note: The publisher’s version of record is available at https://doi.org/10.3892/mmr.2018.8790
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000430556800125
Owner Institution: FSU
Is Part Of: Molecular Medicine Reports.
1791-2997
Issue: iss. 5, vol. 17

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Zhou, X., Natino, D., Zhai, X., Gao, Z., & He, X. (2018). Microrna-22 Inhibits The Proliferation And Migration, And Increases The Cisplatin Sensitivity, Of Osteosarcoma Cells. Molecular Medicine Reports. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000430556800125