You are here

Half-bridge Component Kar1 Promotes Centrosome Separation And Duplication During Budding Yeast Meiosis

Title: The Half-bridge Component Kar1 Promotes Centrosome Separation And Duplication During Budding Yeast Meiosis.
0 views
0 downloads
Name(s): Agarwal, Meenakshi, author
Jin, Hui, author
McClain, Melainia, author
Fan, Jinbo, author
Koch, Bailey A., author
Jaspersen, Sue L., author
Yu, Hong-Guo, author
Type of Resource: text
Genre: Journal Article
Text
Journal Article
Date Issued: 2018-08-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The budding yeast centrosome, often called the spindle pole body (SPB), nudeates microtubules for chromosome segregation during cell division. An appendage, called the half bridge, attaches to one side of the SPB and regulates SPB duplication and separation. Like DNA, the SPB is duplicated only once per cell cycle. During meiosis, however, after one round of DNA replication, two rounds of SPB duplication and separation are coupled with homologue segregation in meiosis I and sister-chromatid segregation in meiosis II. How SPB duplication and separation are regulated during meiosis remains to be elucidated, and whether regulation in meiosis differs from that in mitosis is unclear. Here we show that overproduction of the half-bridge component Kar1 leads to premature SPB separation during meiosis. Furthermore, excessive Kar1 induces SPB overduplication to form supernumerary SPBs, leading to chromosome missegregation and erroneous ascospore formation. Kar1-mediated SPB duplication bypasses the requirement of dephosphorylation of Sfi1, another half-bridge component previously identified as a licensing factor. Our results therefore reveal an unexpected role of Kar1 in licensing meiotic SPB duplication and suggest a unique mechanism of SPB regulation during budding yeast meiosis.
Identifier: FSU_libsubv1_wos_000446089500003 (IID), 10.1091/mbc.E18-03-0163 (DOI)
Keywords: binding, cell-cycle, kinase, saccharomyces-cerevisiae, spindle pole body, protein, chromosome segregation, mitotic spindle, plaques, sporulation
Publication Note: The publisher’s version of record is available at https://doi.org/10.1091/mbc.E18-03-0163
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000446089500003
Owner Institution: FSU
Is Part Of: Molecular Biology of the Cell.
1059-1524
Issue: iss. 15, vol. 29

Choose the citation style.
Agarwal, M., Jin, H., McClain, M., Fan, J., Koch, B. A., Jaspersen, S. L., & Yu, H. -G. (2018). The Half-bridge Component Kar1 Promotes Centrosome Separation And Duplication During Budding Yeast Meiosis. Molecular Biology Of The Cell. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000446089500003