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Spindly Is Required For Rapid Migration Of Human Cells

Title: Spindly Is Required For Rapid Migration Of Human Cells.
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Name(s): Conte, Claudia, author
Baird, Michelle A., author
Davidson, Michael W., author
Griffis, Eric R., author
Type of Resource: text
Genre: Journal Article
Text
Journal Article
Date Issued: 2018-05-01
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Dynein is the sole processive minus-end-directed microtubule motor found in animals. It has roles in cell division, membrane trafficking, and cell migration. Together with dynactin, dynein regulates centrosomal orientation to establish and maintain cell polarity, controls focal adhesion turnover and anchors microtubules at the leading edge. In higher eukaryotes, dynein/dynactin requires additional components such as Bicaudal D to form an active motor complex and for regulating its cellular localization. Spindly is a protein that targets dynein/dynactin to kinetochores in mitosis and can activate its motility in vitro. However, no role for Spindly in interphase dynein/dynactin function has been found. We show that Spindly binds to the cell cortex and microtubule tips and colocalizes with dynein/dynactin at the leading edge of migrating U2OS cells and primary fibroblasts. U2OS cells that lack Spindly migrated slower in 2D than control cells, although centrosome polarization appeared to happen properly in the absence of Spindly. Re-expression of Spindly rescues migration, but the expression of a mutant, which is defective for dynactin binding, failed to rescue this defect. Taken together, these data demonstrate that Spindly plays an important role in mediating a subset of dynein/dynactin's function in cell migration.
Identifier: FSU_libsubv1_wos_000434210800009 (IID), 10.1242/bio.033233 (DOI)
Keywords: Migration, actin, endothelial-cells, cytoplasmic dynein, dynactin, Dynein/Dynactin, end tracking, focal adhesion dynamics, Kinetochore, leading-edge, lissencephaly gene, microtubule-organizing centers, plus ends
Publication Note: The publisher’s version of record is available at https://doi.org/10.1242/bio.033233
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000434210800009
Owner Institution: FSU
Is Part Of: Biology Open.
2046-6390
Issue: iss. 5, vol. 7

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Conte, C., Baird, M. A., Davidson, M. W., & Griffis, E. R. (2018). Spindly Is Required For Rapid Migration Of Human Cells. Biology Open. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000434210800009