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Cell Adhesion and Proliferation on the “Living” Surface of a Polyelectrolyte Multilayer

Title: Cell Adhesion and Proliferation on the “Living” Surface of a Polyelectrolyte Multilayer.
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Name(s): Arias, Carlos B., author
Surmaitis, Richard L., author
Schlenoff, Joseph B., author
Type of Resource: text
Genre: Text
Journal Article
Date Issued: 2016-05-18
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The adhesion of living eukaryotic cells to a substrate, one of the most complex problems in surface science, requires adsorption of extracellular proteins such as fibronectin. Thin films of polyelectrolyte complex made layer-by-layer (polyelectrolyte multilayers or PEMUs) offer a high degree of control of surface charge and composition - interconnected and essential variables for protein adhesion. Fibroblasts grown on multilayers of poly(styrene sulfonate), PSS, and poly(diallyldimethylammonium), PDADMA, with increasing thickness exhibit good adhesion until the 12th layer of polyelectrolyte has been added, whereupon there is a sudden transition to nonadhesive behavior. This sharp change is due to the migration of excess positive charge to the surface – a previously unrecognized property of PEMUs. Precise radiotracer assays of adsorbed 125I-albumin, show how protein adsorption is related to multilayer surface charge. With more negative surface charge density from the sulfonates of PSS, more albumin adsorbs to the surface. However, a loosely-held or “soft corona” exchanges with serum protein under the Vroman effect, which is correlated with poor cell adhesion. A comprehensive view of cell adhesion highlights the central role of robust protein adhesion, which is required before any secondary effects of matrix stiffness on cell fate can come into play.
Identifier: FSU_libsubv1_scholarship_submission_1540238895_2ac07ed1 (IID), 10.1021/acs.langmuir.6b00784 (DOI)
Keywords: biointerface, adhesion mechanism, surface charge, cell culture, interface, adsorption, stiffness, durotaxis, albumin
Publication Note: This document is the Accepted Manuscript version of a Published Work that appeared in final form in Langmuir, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.langmuir.6b00784.
Grant Number: DMR-1207188 (NSF), DMR-1506824 (NSF)
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1540238895_2ac07ed1
Owner Institution: FSU
Is Part Of: Langmuir.

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Arias, C. B., Surmaitis, R. L., & Schlenoff, J. B. (2016). Cell Adhesion and Proliferation on the “Living” Surface of a Polyelectrolyte Multilayer. Langmuir. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1540238895_2ac07ed1