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Promoter Interactome Of Human Embryonic Stem Cell-derived Cardiomyocytes Connects Gwas Regions To Cardiac Gene Networks

Title: Promoter Interactome Of Human Embryonic Stem Cell-derived Cardiomyocytes Connects Gwas Regions To Cardiac Gene Networks.
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Name(s): Choy, Mun-Kit, author
Javierre, Biola M., author
Williams, Simon G., author
Baross, Stephanie L., author
Liu, Yingjuan, author
Wingett, Steven W., author
Akbarov, Artur, author
Wallace, Chris, author
Freire-Pritchett, Paula, author
Rugg-Gunn, Peter J., author
Spivakov, Mikhail, author
Fraser, Peter, author
Keavney, Bernard D., author
Type of Resource: text
Genre: Journal Article
Text
Journal Article
Date Issued: 2018-06-28
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Long-range chromosomal interactions bring distal regulatory elements and promoters together to regulate gene expression in biological processes. By performing promoter capture Hi-C (PCHi-C) on human embryonic stem cell-derived cardiomyocytes (hESC-CMs), we show that such promoter interactions are a key mechanism by which enhancers contact their target genes after hESC-CM differentiation from hESCs. We also show that the promoter interactome of hESC-CMs is associated with expression quantitative trait loci (eQTLs) in cardiac left ventricular tissue; captures the dynamic process of genome reorganisation after hESC-CM differentiation; overlaps genome-wide association study (GWAS) regions associated with heart rate; and identifies new candidate genes in such regions. These findings indicate that regulatory elements in hESC-CMs identified by our approach control gene expression involved in ventricular conduction and rhythm of the heart. The study of promoter interactions in other hESC-derived cell types may be of utility in functional investigation of GWAS-associated regions.
Identifier: FSU_libsubv1_wos_000436548700017 (IID), 10.1038/s41467-018-04931-0 (DOI)
Keywords: disease, expression, channels, genome-wide association, capture hi-c, heart, endothelin receptor, enhancers, loci, regulatory elements
Publication Note: The publisher’s version of record is available at https://doi.org/10.1038/s41467-018-04931-0
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000436548700017
Owner Institution: FSU
Is Part Of: Nature Communications.
2041-1723
Issue: vol. 9

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Choy, M. -K., Javierre, B. M., Williams, S. G., Baross, S. L., Liu, Y., Wingett, S. W., … Keavney, B. D. (2018). Promoter Interactome Of Human Embryonic Stem Cell-derived Cardiomyocytes Connects Gwas Regions To Cardiac Gene Networks. Nature Communications. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_wos_000436548700017