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Top-down and Middle-down Proteomics by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Title: Top-down and Middle-down Proteomics by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.
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Inaccessible until Sep 27, 2020 due to copyright restrictions.

Name(s): Jiang, Tingting, author
Marshall, Alan G. (Alan George), 1944-, professor directing dissertation
Tang, Hengli, university representative
Dorsey, John G., committee member
Yang, Wei, committee member
Florida State University, degree granting institution
College of Arts and Sciences, degree granting college
Department of Chemistry and Biochemistry, degree granting department
Type of Resource: text
Genre: Text
Doctoral Thesis
Issuance: monographic
Date Issued: 2017
Publisher: Florida State University
Place of Publication: Tallahassee, Florida
Physical Form: computer
online resource
Extent: 1 online resource (133 pages)
Language(s): English
Abstract/Description: Mass spectrometry (MS) has become an important analytical method for proteomic research due to its high mass accuracy, resolution, and selectivity. Even though the traditional bottom-up MS-based method is still a widespread routine for proteomic analysis, middle- and top-down approaches should provide more comprehensive characterization of proteins isoforms and post-translational modifications (PTMs) due to their capabilities of maintaining the connectivity between modifications. The Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer equipped with multiple efficient fragmentation techniques offers the ultrahigh mass accuracy and resolution to enable separation and assignment of overlapped precursor and fragment spectral peaks from extremely complex spectra without ambiguity, which gives us great advantages in middle- and top-down MS-based proteomic analysis.
Identifier: FSU_FALL2017_Jiang_fsu_0071E_14207 (IID)
Submitted Note: A Dissertation submitted to the Department of Chemistry and Biochemistry in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
Degree Awarded: Fall Semester 2017.
Date of Defense: November 6, 2017.
Keywords: FT-ICR, LC-MS, Mass spectrometry, Middle-down, Proteomics, Top-down
Bibliography Note: Includes bibliographical references.
Advisory Committee: Alan G. Marshall, Professor Directing Dissertation; Hengli Tang, University Representative; John G. Dorsey, Committee Member; Wei Yang, Committee Member.
Subject(s): Chemistry, Analytic
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_FALL2017_Jiang_fsu_0071E_14207
Owner Institution: FSU

Choose the citation style.
Jiang, T. (2017). Top-down and Middle-down Proteomics by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Retrieved from http://purl.flvc.org/fsu/fd/FSU_FALL2017_Jiang_fsu_0071E_14207