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Analytical method for protein mapping using hydrogen/deuterium exchange

Title: Analytical method for protein mapping using hydrogen/deuterium exchange.
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Name(s): Marshall, Alan G., inventor
Emmett, Mark R., inventor
Kazazic, Sasa, inventor
Greig, Michael J., inventor
Type of Resource: text
Genre: Utility
Text
Date Created: 2007-02-08
Date Issued: 2009-10-13
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Analytical methods using hydrogen/deuterium exchange are provided which reduce or eliminate the back-exchange of deuterium for hydrogen. The methods, which are useful in protein and peptide mapping, include the steps of (a) providing a peptide or protein comprising a solvent accessible hydrogen; (b) exchanging the solvent accessible hydrogen for a deuterium; (c) separating the peptide or protein with supercritical fluid chromatography; and (d) analyzing by mass spectrometry the mass of the separated peptide or protein. Supercritical fluid chromatography enables the observation of fast exchanging hydrogen atoms missed using conventional liquid chromatography methods.
Identifier: FSU_uspto_7601542 (IID), 7601542 (patent number), 12000 (inventor key), 11/672789 (app id), 6c9256d97d167832cbab694dc904bd27 (assignee id)
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_uspto_7601542
Use and Reproduction: Copyright not evaluated
Owner Institution: FSU

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Analytical method for protein mapping using hydrogen/deuterium exchange. (2009). Analytical method for protein mapping using hydrogen/deuterium exchange. Retrieved from http://purl.flvc.org/fsu/fd/FSU_uspto_7601542