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Bisphenol-A as an estrogen mimic altering chromatin structure

Title: Bisphenol-A as an estrogen mimic altering chromatin structure: Link between environmental toxins and chromatin biochemical state.
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Name(s): Budine, Taylor D, author
Type of Resource: text
Genre: Text
Bachelor Thesis
Date Issued: 2018-04-27
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Steroid hormones are the largest class of regulatory factors in the mammalian biology. Steroid hormones bind hormone receptors, allowing them to dimerize and function as transcription factors. Frequently this regulation of gene transcription is associated with nucleosome redistribution. Bisphenol-A (BPA) is a common toxin found in many of the plasticids and epoxy resins we use every day. BPA is a thought to be an estradiol mimic, binding to hormone receptors allowing them to dimerize and function as transcription factors, which stimulate nucleosome redistribution at inappropriate times. However, nucleosome distribution from BPA exposure has not yet been observed. I expect that as BPA mimics estradiol and dysregulates the potential for gene expression, and that monitoring alterations in chromatin structure will give valuable insights into the first steps of this dysregulation. I mapped nucleosome redistribution and nucleosome sensitivity in both untreated cells and cells exposed to BPA to observe any change in nucleosome repositioning and biochemical composition after this stimulus. If BPA functionally acts as an estradiol mimic and is able to change chromatin structure, untimely or unwanted gene expression may result. BPA will be expected to induce remolding in the cell’s chromatin structure and affect the endocrine responsive genes. My results would show how other stimuli could mimic naturally occurring substances and be unexpected regulatory factors in altering our chromatin structure. BPA is one of many persistent toxins readily in our environment that we are exposed to. We found that there are transient nucleosome positional changes with exposure to Estradiol and Bisphenol-A at the -2, -1, TSS, +1 and +2 positions while comparing the two stimuli at the 10-minute post exposure time period. We are especially interested in the nucleosome loss or gain at the +1 position because of its implications for possible gene expression. These results show that there is indeed similar chromatin remodeling behavior between Estradiol and Bisphenol-A to possibly warrant connections to the potential of the cell. This study will be one of the first to propose chromatin remodeling as the underpinnings to Bisphenol-A ability to mimic the natural sex hormone Estradiol. The scientific world is now becoming more aware of how environmental factors and pollutants all around us can have an acute or trans-generational effect on our health and epigenomic regulation.
Identifier: FSU_libsubv1_scholarship_submission_1524847528_f54529a4 (IID)
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1524847528_f54529a4
Owner Institution: FSU

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Budine, T. D. (2018). Bisphenol-A as an estrogen mimic altering chromatin structure: Link between environmental toxins and chromatin biochemical state. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1524847528_f54529a4