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Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein

Title: Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein.
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Name(s): Sakano, Hitomi, author
Zorio, Diego A.R., author
Wang, Xiaoyu, author
Ting, Ying S., author
Noble, William S., author
MacCoss, Michael J., author
Rubel, Edwin W, author
Wang, Yuan, author
Type of Resource: text
Genre: Text
Journal Article
Date Issued: 2017-07-24
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The avian nucleus laminaris (NL) is a brainstem nucleus necessary for binaural processing, analogous in structure and function to the mammalian medial superior olive. In chickens (Gallus gallus), NL is a well-studied model system for activity-dependent neural plasticity. Its neurons have bipolar extension of dendrites, which receive segregated inputs from two ears and display rapid and compartment-specific reorganization in response to unilateral changes in auditory input. More recently, FMRP (fragile X mental retardation protein), an RNA-binding protein that regulates local protein translation, has been shown to be enriched in NL dendrites, suggesting its potential role in the structural dynamics of these dendrites. To explore the molecular role of FMRP in this nucleus, we performed proteomic analysis of NL, using micro laser capture and liquid chromatography tandem mass spectrometry. We identified 657 proteins, greatly represented in pathways involved in mitochondria, translation and metabolism, consistent with high levels of activity of NL neurons. Of these, 94 are potential FMRP targets, by comparative analysis with previously proposed FMRP targets in mammals. These proteins are enriched in pathways involved in cellular growth, cellular trafficking and transmembrane transport. Immunocytochemistry verified the dendritic localization of several proteins in NL. Furthermore, we confirmed the direct interaction of FMRP with one candidate, RhoC, by in vitro RNA binding assays. In summary, we provide a database of highly expressed proteins in NL and in particular a list of potential FMRP targets, with the goal of facilitating molecular characterization of FMRP signaling in future studies.
Identifier: FSU_libsubv1_scholarship_submission_1511841485_a5846c5a_P (IID), 10.1002/cne.24281 (DOI)
Keywords: Autism spectrum disorders, Gene ontology, RNA binding protein, Dendritic plasticity, Cytoskeletal proteins
Publication Note: The original published version can be found at http://doi.org/10.1002/cne.24281.
Preferred Citation: Sakano H, Zorio DAR, Wang X, Ting YS, Noble WS, MacCoss MJ, Rubel EW, Wang Y. Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein. J Comp Neurol. 2017 Jul 7. doi: 10.1002/cne.24281. [Epub ahead of print] PMID: 28685837
Grant Number: NIDCD Grants DC000018, DC013074, DC03829; NIGMS Grants P41 GM103533; NIGMS R01 GM121818, and the Genentech Advanced Neurodegenerative Disease Research grant
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1511841485_a5846c5a_P
Host Institution: FSU
Is Part Of: Journal of Comparative Neurology.
Issue: iss. 15, vol. 525

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Sakano, H., Zorio, D. A. R., Wang, X., Ting, Y. S., Noble, W. S., MacCoss, M. J., … Wang, Y. (2017). Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein. Journal Of Comparative Neurology. Retrieved from http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1511841485_a5846c5a_P

Title: Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein.
Name(s): Sakano, Hitomi, author
Zorio, Diego A.R., author
Wang, Xiaoyu, author
Ting, Ying S., author
Noble, William S., author
MacCoss, Michael J., author
Rubel, Edwin W, author
Wang, Yuan, author
Type of Resource: text
Genre: Text
Journal Article
Date Issued: 2017-07-24
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: The avian nucleus laminaris (NL) is a brainstem nucleus necessary for binaural processing, analogous in structure and function to the mammalian medial superior olive. In chickens (Gallus gallus), NL is a well-studied model system for activity-dependent neural plasticity. Its neurons have bipolar extension of dendrites, which receive segregated inputs from two ears and display rapid and compartment-specific reorganization in response to unilateral changes in auditory input. More recently, FMRP (fragile X mental retardation protein), an RNA-binding protein that regulates local protein translation, has been shown to be enriched in NL dendrites, suggesting its potential role in the structural dynamics of these dendrites. To explore the molecular role of FMRP in this nucleus, we performed proteomic analysis of NL, using micro laser capture and liquid chromatography tandem mass spectrometry. We identified 657 proteins, greatly represented in pathways involved in mitochondria, translation and metabolism, consistent with high levels of activity of NL neurons. Of these, 94 are potential FMRP targets, by comparative analysis with previously proposed FMRP targets in mammals. These proteins are enriched in pathways involved in cellular growth, cellular trafficking and transmembrane transport. Immunocytochemistry verified the dendritic localization of several proteins in NL. Furthermore, we confirmed the direct interaction of FMRP with one candidate, RhoC, by in vitro RNA binding assays. In summary, we provide a database of highly expressed proteins in NL and in particular a list of potential FMRP targets, with the goal of facilitating molecular characterization of FMRP signaling in future studies.
Identifier: FSU_libsubv1_scholarship_submission_1511841485_a5846c5a_Base (IID), 10.1002/cne.24281 (DOI)
Keywords: Autism spectrum disorders, Gene ontology, RNA binding protein, Dendritic plasticity, Cytoskeletal proteins
Publication Note: The original published version can be found at http://doi.org/10.1002/cne.24281.
Preferred Citation: Sakano H, Zorio DAR, Wang X, Ting YS, Noble WS, MacCoss MJ, Rubel EW, Wang Y. Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein. J Comp Neurol. 2017 Jul 7. doi: 10.1002/cne.24281. [Epub ahead of print] PMID: 28685837
Grant Number: NIDCD Grants DC000018, DC013074, DC03829; NIGMS Grants P41 GM103533; NIGMS R01 GM121818, and the Genentech Advanced Neurodegenerative Disease Research grant
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_libsubv1_scholarship_submission_1511841485_a5846c5a_Base
Host Institution: FSU
Is Part Of: Journal of Comparative Neurology.
Issue: iss. 15, vol. 525