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Distinct Neural Properties in the Low-Frequency Region of the Chicken Cochlear Nucleus Magnocellularis.

Title: Distinct Neural Properties in the Low-Frequency Region of the Chicken Cochlear Nucleus Magnocellularis.
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Name(s): Wang, Xiaoyu, author
Hong, Hui, author
Brown, David H, author
Sanchez, Jason Tait, author
Wang, Yuan, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-04-11
Physical Form: computer
online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Topography in the avian cochlear nucleus magnocellularis (NM) is represented as gradually increasing characteristic frequency (CF) along the caudolateral-to-rostromedial axis. In this study, we characterized the organization and cell biophysics of the caudolateral NM (NMc) in chickens (Gallus gallus). Examination of cellular and dendritic architecture first revealed that NMc contains small neurons and extensive dendritic processes, in contrast to adendritic, large neurons located more rostromedially. Individual dye-filling study further demonstrated that NMc is divided into two subregions, with NMc2 neurons having larger and more complex dendritic fields than NMc1. Axonal tract tracing studies confirmed that NMc1 and NMc2 neurons receive afferent inputs from the auditory nerve and the superior olivary nucleus, similar to the adendritic NM. However, the auditory axons synapse with NMc neurons via small bouton-like terminals, unlike the large end bulb synapses on adendritic NM neurons. Immunocytochemistry demonstrated that most NMc2 neurons express cholecystokinin but not calretinin, distinct from NMc1 and adendritic NM neurons that are cholecystokinin negative and mostly calretinin positive. Finally, whole-cell current clamp recordings revealed that NMc neurons require significantly lower threshold current for action potential generation than adendritic NM neurons. Moreover, in contrast to adendritic NM neurons that generate a single-onset action potential, NMc neurons generate multiple action potentials to suprathreshold sustained depolarization. Taken together, our data indicate that NMc contains multiple neuron types that are structurally, connectively, molecularly, and physiologically different from traditionally defined NM neurons, emphasizing specialized neural properties for processing low-frequency sounds.
Identifier: FSU_pmch_28413822 (IID), 10.1523/ENEURO.0016-17.2017 (DOI), PMC5388668 (PMCID), 28413822 (RID), 28413822 (EID), eN-NWR-0016-17 (PII)
Keywords: Action potential, Calcium binding protein, Cholecystokinin, Low frequency processing, Neural excitability
Grant Number: R03 DC013841
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388668.
Subject(s): 2-Amino-5-phosphonovalerate/pharmacology
6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology
Animals
Animals, Newborn
Auditory Pathways/physiology
Calbindin 2/metabolism
Chick Embryo
Chickens
Cholecystokinin/metabolism
Cochlear Nucleus/cytology
Cochlear Nucleus/embryology
Cochlear Nucleus/growth & development
Dendrites/physiology
Excitatory Amino Acid Antagonists/pharmacology
Female
GABA Antagonists/pharmacology
Imaging, Three-Dimensional
In Vitro Techniques
Male
Membrane Potentials/drug effects
Membrane Potentials/physiology
Microtubule-Associated Proteins/metabolism
Neurons/cytology
Neurons/physiology
Parvalbumins/metabolism
Patch-Clamp Techniques
Picrotoxin/pharmacology
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28413822
Owner Institution: FSU
Is Part Of: eNeuro.
2373-2822
Issue: iss. 2, vol. 4

Choose the citation style.
Wang, X., Hong, H., Brown, D. H., Sanchez, J. T., & Wang, Y. (2017). Distinct Neural Properties in the Low-Frequency Region of the Chicken Cochlear Nucleus Magnocellularis. Eneuro. Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28413822