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In Vitro and In Vivo Evaluation of a Three-Dimensional Porous Multi-Walled Carbon Nanotube Scaffold for Bone Regeneration.

Title: In Vitro and In Vivo Evaluation of a Three-Dimensional Porous Multi-Walled Carbon Nanotube Scaffold for Bone Regeneration.
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Name(s): Tanaka, Manabu, author
Sato, Yoshinori, author
Zhang, Mei, author
Haniu, Hisao, author
Okamoto, Masanori, author
Aoki, Kaoru, author
Takizawa, Takashi, author
Yoshida, Kazushige, author
Sobajima, Atsushi, author
Kamanaka, Takayuki, author
Kato, Hiroyuki, author
Saito, Naoto, author
Type of Resource: text
Genre: Journal Article
Text
Date Issued: 2017-02-17
Physical Form: computer
Physical Form: online resource
Extent: 1 online resource
Language(s): English
Abstract/Description: Carbon nanotubes (CNTs) have attracted a great deal of attention for the biological and medical science fields because of their characteristic physical and biological properties. In this study, we investigated the capacity of the 3D porous CNT scaffold (CNT porous block; CNTp) for bone regenerative medicine. Surface observations using a scanning electron microscope (SEM), crystal depositions on the surface of CNTps immersed in simulated body fluid (SBF), and evaluations of protein adsorption and controlled releasing were conducted to assess physical properties. The cell proliferation and cell morphology were observed using SEM and fluorescent microscopy. CNTps were implanted into critical-size mouse calvarial defects and evaluated for their osteoconductive ability and in vivo controlled release of recombinant human BMP-2 (rhBMP-2). Interconnected porous HA ceramics (IP-CHAs) were used for comparison. CNTps have multiporous structures with interporous connections with networks of multiwalled CNTs. Crystals containing calcium and phosphate were deposited in CNTps and on the surface of the CNT networks by immersing CNTps in SBF. CNTps adsorbed more significantly and released protein more gradually than IP-CHAs. Preosteoblasts seeded onto CNTps filled pores with stretched actin filaments and filopodia. Compared with IP-CHAs, CNTps showed significantly higher cell proliferation, better osteoconduction, and more bone generation with rhBMP-2. In this study, CNTps demonstrated good osteoconductive ability, cell attachment and proliferation capacity, and growth factor retaining ability. CNTps have the potential not only as artificial bones for the treatment of bone defects, but also as scaffolds for regenerative medicine using tissue engineering approaches.
Identifier: FSU_pmch_28336879 (IID), 10.3390/nano7020046 (DOI), PMC5333031 (PMCID), 28336879 (RID), 28336879 (EID), nano7020046 (PII)
Keywords: Bone regeneration, Carbon nanotube, Tissue engineering
Publication Note: This NIH-funded author manuscript originally appeared in PubMed Central at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333031.
Persistent Link to This Record: http://purl.flvc.org/fsu/fd/FSU_pmch_28336879
Owner Institution: FSU
Is Part Of: Nanomaterials (Basel, Switzerland).
2079-4991
Issue: iss. 2, vol. 7

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Tanaka, M., Sato, Y., Zhang, M., Haniu, H., Okamoto, M., Aoki, K., … Saito, N. (2017). In Vitro and In Vivo Evaluation of a Three-Dimensional Porous Multi-Walled Carbon Nanotube Scaffold for Bone Regeneration. Nanomaterials (Basel, Switzerland). Retrieved from http://purl.flvc.org/fsu/fd/FSU_pmch_28336879